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The map label for this gene is nifN [H]

Identifier: 83310667

GI number: 83310667

Start: 1695856

End: 1697214

Strand: Reverse

Name: nifN [H]

Synonym: amb1568

Alternate gene names: 83310667

Gene position: 1697214-1695856 (Counterclockwise)

Preceding gene: 83310668

Following gene: 83310666

Centisome position: 34.17

GC content: 68.29

Gene sequence:

>1359_bases
ATGGCCCTTATCGTCGACCACAACAAGGCGCTTTCCACCTCTCCGCTCAAGGTCAGCGCGCCGCTGGGCGCGGCGCTGGC
CTTCATGGGGATGAAGGGCTGCCTGCCCATGTTCCACGGCTCCCAGGGCTGCACCGCCTTCGCCCTGGTGATGATGGTCC
GCCACTTCCGCGAGGCCATCCCGCTGCAGACCACCGCCATGAACGAGATCAGCACCATCCTGGGTGGCATGGACCAGGTG
GAGGAAGGTCTCCTCAACATCGCCAAGCGCGCCAAGCCGGAAATCATCGGCCTGATCTCCACCGCCCTGACCGAGACCCG
CGGCGAAGACATGGCCGGCGACGTCAAGGTCATTCGGGCACGCAACAAGGAACTGGCCGATATCGCCGTGGTCGACGTCT
CGGCCCCCGACTTCTCGGGCTCGCTGGAGACCGGCTGGGGCAAGGCGGTGACCGCCATCATCAAGAGCCTGGCCAAGCCG
TCCGGCAACCACGCCAAGCTGGACCGGGTCAACCTGCTGCCCGGCGCCCATCTCACCCCCGGTGACGTGGAGGCCTTAAA
GGACATCATCGCCGATTTCGGCCTGGAGGCCATGGTCCTGCCCGACCTGTCGGACTCCCTGGACGGCCATGTGCCCGACG
CCTATACCCCCACCACCCTGGGCGGCACCGCCGTCGCCCAGGTGCGCGAGATGGGCCGGGCACGCCTGACCATCGCCATC
GGCGAGCACATGCGCATCGCCGCCAAGACCGCCGAGACCATCACCGGCGTTCCCTCGGTGGTGCTGGACCGGGTCACCGG
CCTGGATGCCGTCGACCGCCTGATGGTCTGCCTGTCCACCGTGGCCGGCGTTCCCGTTCCCGCCCGCCAGCGCCGCGCCC
GCTCGCAGCTGGTGGACGCCATGCTGGACGGCCACTTCTATTTCGGCGGCCGCTCCATCGCCATCGCCGCCGAGCCCGCC
TTGCTGTGGAGCATGGGCAAGCTGGCCACCGACATGGGCGCCACCGTCGCGGCGGCGGTCACCACCCAGCCCGTCCCCAT
GCTGGCCGAGCTGAAGGCCGAACGGATCATCGTCGGCGATTTCGAGGATCTGGAGGACCAGATCCTGGAGGCCGGCGGCG
CCGACCTGATCATCGCCAATTCCAATGGACGTCAGGTGGCCGCCCATCACGGCATCCCGCTGTTCCGCGCCGGCTTTCCG
GTGTTCGACCGCCTGGGCGCCGCCCACCTGACATCGGCGGGTTACCGCGGCACGCGGGACCTGATCACCCAATTGGGCAA
CATCCTGATGGAGCGCCCCGGACACGCCCACCAGCAGTCGGAGCACGCCCATGGTCACCAGAGTGCTGCGGCTGGTTGA

Upstream 100 bases:

>100_bases
CCTATGACGGCATGGTGGCCCTGGTGCGCGAGATCGATCTTTCCATCAACAGCCCGATCTGGGCGCAGGTCCGCGCCCCC
GCCCCCTGGGAGAATTCGTG

Downstream 100 bases:

>100_bases
TCCCGCCGCGCCCCCTTCCCAGGGATCGAGACCGAAGATCAAAGGAGGTCACATGAGAATCGCCGTCGCCACCCAGGACC
GCCAGCGCGTCGACGCCCAC

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MALIVDHNKALSTSPLKVSAPLGAALAFMGMKGCLPMFHGSQGCTAFALVMMVRHFREAIPLQTTAMNEISTILGGMDQV
EEGLLNIAKRAKPEIIGLISTALTETRGEDMAGDVKVIRARNKELADIAVVDVSAPDFSGSLETGWGKAVTAIIKSLAKP
SGNHAKLDRVNLLPGAHLTPGDVEALKDIIADFGLEAMVLPDLSDSLDGHVPDAYTPTTLGGTAVAQVREMGRARLTIAI
GEHMRIAAKTAETITGVPSVVLDRVTGLDAVDRLMVCLSTVAGVPVPARQRRARSQLVDAMLDGHFYFGGRSIAIAAEPA
LLWSMGKLATDMGATVAAAVTTQPVPMLAELKAERIIVGDFEDLEDQILEAGGADLIIANSNGRQVAAHHGIPLFRAGFP
VFDRLGAAHLTSAGYRGTRDLITQLGNILMERPGHAHQQSEHAHGHQSAAAG

Sequences:

>Translated_452_residues
MALIVDHNKALSTSPLKVSAPLGAALAFMGMKGCLPMFHGSQGCTAFALVMMVRHFREAIPLQTTAMNEISTILGGMDQV
EEGLLNIAKRAKPEIIGLISTALTETRGEDMAGDVKVIRARNKELADIAVVDVSAPDFSGSLETGWGKAVTAIIKSLAKP
SGNHAKLDRVNLLPGAHLTPGDVEALKDIIADFGLEAMVLPDLSDSLDGHVPDAYTPTTLGGTAVAQVREMGRARLTIAI
GEHMRIAAKTAETITGVPSVVLDRVTGLDAVDRLMVCLSTVAGVPVPARQRRARSQLVDAMLDGHFYFGGRSIAIAAEPA
LLWSMGKLATDMGATVAAAVTTQPVPMLAELKAERIIVGDFEDLEDQILEAGGADLIIANSNGRQVAAHHGIPLFRAGFP
VFDRLGAAHLTSAGYRGTRDLITQLGNILMERPGHAHQQSEHAHGHQSAAAG
>Mature_451_residues
ALIVDHNKALSTSPLKVSAPLGAALAFMGMKGCLPMFHGSQGCTAFALVMMVRHFREAIPLQTTAMNEISTILGGMDQVE
EGLLNIAKRAKPEIIGLISTALTETRGEDMAGDVKVIRARNKELADIAVVDVSAPDFSGSLETGWGKAVTAIIKSLAKPS
GNHAKLDRVNLLPGAHLTPGDVEALKDIIADFGLEAMVLPDLSDSLDGHVPDAYTPTTLGGTAVAQVREMGRARLTIAIG
EHMRIAAKTAETITGVPSVVLDRVTGLDAVDRLMVCLSTVAGVPVPARQRRARSQLVDAMLDGHFYFGGRSIAIAAEPAL
LWSMGKLATDMGATVAAAVTTQPVPMLAELKAERIIVGDFEDLEDQILEAGGADLIIANSNGRQVAAHHGIPLFRAGFPV
FDRLGAAHLTSAGYRGTRDLITQLGNILMERPGHAHQQSEHAHGHQSAAAG

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005975 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 47584; Mature: 47452

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVDHNKALSTSPLKVSAPLGAALAFMGMKGCLPMFHGSQGCTAFALVMMVRHFREAI
CEEEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHC
PLQTTAMNEISTILGGMDQVEEGLLNIAKRAKPEIIGLISTALTETRGEDMAGDVKVIRA
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHEEEC
RNKELADIAVVDVSAPDFSGSLETGWGKAVTAIIKSLAKPSGNHAKLDRVNLLPGAHLTP
CCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
GDVEALKDIIADFGLEAMVLPDLSDSLDGHVPDAYTPTTLGGTAVAQVREMGRARLTIAI
CHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEE
GEHMRIAAKTAETITGVPSVVLDRVTGLDAVDRLMVCLSTVAGVPVPARQRRARSQLVDA
CCCCEEHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MLDGHFYFGGRSIAIAAEPALLWSMGKLATDMGATVAAAVTTQPVPMLAELKAERIIVGD
HHCCEEEECCEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHCCCEEEECC
FEDLEDQILEAGGADLIIANSNGRQVAAHHGIPLFRAGFPVFDRLGAAHLTSAGYRGTRD
HHHHHHHHHHCCCCEEEEECCCCCEEEHHCCCCHHHCCCCHHHHCCHHHHHHCCCCCHHH
LITQLGNILMERPGHAHQQSEHAHGHQSAAAG
HHHHHHHHHHCCCCCCHHCCHHCCCCCCCCCC
>Mature Secondary Structure 
ALIVDHNKALSTSPLKVSAPLGAALAFMGMKGCLPMFHGSQGCTAFALVMMVRHFREAI
EEEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHC
PLQTTAMNEISTILGGMDQVEEGLLNIAKRAKPEIIGLISTALTETRGEDMAGDVKVIRA
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHEEEC
RNKELADIAVVDVSAPDFSGSLETGWGKAVTAIIKSLAKPSGNHAKLDRVNLLPGAHLTP
CCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
GDVEALKDIIADFGLEAMVLPDLSDSLDGHVPDAYTPTTLGGTAVAQVREMGRARLTIAI
CHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEE
GEHMRIAAKTAETITGVPSVVLDRVTGLDAVDRLMVCLSTVAGVPVPARQRRARSQLVDA
CCCCEEHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MLDGHFYFGGRSIAIAAEPALLWSMGKLATDMGATVAAAVTTQPVPMLAELKAERIIVGD
HHCCEEEECCEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHCCCEEEECC
FEDLEDQILEAGGADLIIANSNGRQVAAHHGIPLFRAGFPVFDRLGAAHLTSAGYRGTRD
HHHHHHHHHHCCCCEEEEECCCCCEEEHHCCCCHHHCCCCHHHHCCHHHHHHCCCCCHHH
LITQLGNILMERPGHAHQQSEHAHGHQSAAAG
HHHHHHHHHHCCCCCCHHCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]