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The map label for this gene is nifV [H]

Identifier: 83310661

GI number: 83310661

Start: 1692260

End: 1693405

Strand: Reverse

Name: nifV [H]

Synonym: amb1562

Alternate gene names: 83310661

Gene position: 1693405-1692260 (Counterclockwise)

Preceding gene: 83310662

Following gene: 83310660

Centisome position: 34.09

GC content: 69.72

Gene sequence:

>1146_bases
ATGACGAAGGCCGCCACCATCGTCATGAACGATACGACTCTGAGGGATGGAGAGCAGACCGCCGGGGTCGCGTTCCGCCT
GGACGAGAAGCTGGATATCGCCCGGACGCTGGACATCGCCGGCGTACCGGAGATCGAGGTGGGCATTCCCGCCATGGGAG
CCGAGGAACAGGCCGCCATCCGCAAGGTGGCGGGCCTCGGCCTCAAGGCCCGGCTGATCGCCTGGTGCCGGATGATGGAA
GACGACCTGGAGGCGGCGGCGCTGTGCGACGTCGCCACCGTCAACCTGTCCATTCCGGTCTCCGACCTGCAACTGGCCGC
CAAGATGGGCCGCGACCGCGCCTGGGCGCTGGAGCATATCCGCGTCATGGTGGGCAAGGCCCGCGCCATGGGCTTTGACG
TCCTGGTGGGCGGCGAGGACAGCTCGCGCGCCGATCCGGCCTTCCTGGCCGATGTGGTGCGGGCCTGCGAGGCGGCGGGC
GCCCAGCGCTTCCGCTTCGCCGATACCATGGGGATCATGGACCCCTTCGCCGTGCACGACGCCTTCCGCGCCCTGCGCGC
CGCCACCTCCATGGAACTAGAAATTCACGCCCACGACGACCTGGGGCTGGCCACCGCCAATTCCCTGGCCGCCGTCCGGG
GCGGCGCCACGCATGTGAGCACCACGGTCAACGGCCTGGGCGAGCGGGCCGGCAACGCCCCCCTGGAAGAGGTGGTGGTG
GCGCTGGGCCACCTCTACGGCCGGGAAACCGGCATCGACAAGCGCCAGTTGGGCCGGGTGTCCAAGCTGGTGGCCGAGGC
CTCGGGCCGCCCGGTGCCGGTGGACAAGAGCATCGTCGGCGAGAACATCTTCACCCACGAATCCGGCATCCATGTCAGCG
GCCTGCTGCGCGATCCCCGCACCTATCAGGCGCTAGACCCGGCCGAGTTGGGCCGCGGCCACCGTCTGGTGCTGGGCAAG
CATTCGGGCCTGACCTCGGTGCTGCACGCCTGCCGCGAGATCGGGCTGGAGGTGGATGCGGCGAAAGCCCGCGCCATGCT
GGCCCAGGTCCGCCGCCACGCCTCGACCACCAAGACCATCCCCACCGCAGACGATTTGCGGCGTTTCGCCGGAACCGCCA
CCGCCATCACCGGAGTCCCGTCATGA

Upstream 100 bases:

>100_bases
GCTTCGTGTTCGACAATCCCAACCAGCCCCCCGCCCCCACCACCACCGCCGGCGGCGGCTGTTCCTGCGCCATGAAGAGC
TGCGGCTAGGAGACCCCGCC

Downstream 100 bases:

>100_bases
TCCTCGAGACCCTCGCCACCCTGTCGTCCGCCGAGCAGTTCTTCCAGACCCTGCAGGTTCCCTTCGACCAGTCGGTGCTC
AACGTCAGCCGCCTGCACAT

Product: isopropylmalate/homocitrate/citramalate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTKAATIVMNDTTLRDGEQTAGVAFRLDEKLDIARTLDIAGVPEIEVGIPAMGAEEQAAIRKVAGLGLKARLIAWCRMME
DDLEAAALCDVATVNLSIPVSDLQLAAKMGRDRAWALEHIRVMVGKARAMGFDVLVGGEDSSRADPAFLADVVRACEAAG
AQRFRFADTMGIMDPFAVHDAFRALRAATSMELEIHAHDDLGLATANSLAAVRGGATHVSTTVNGLGERAGNAPLEEVVV
ALGHLYGRETGIDKRQLGRVSKLVAEASGRPVPVDKSIVGENIFTHESGIHVSGLLRDPRTYQALDPAELGRGHRLVLGK
HSGLTSVLHACREIGLEVDAAKARAMLAQVRRHASTTKTIPTADDLRRFAGTATAITGVPS

Sequences:

>Translated_381_residues
MTKAATIVMNDTTLRDGEQTAGVAFRLDEKLDIARTLDIAGVPEIEVGIPAMGAEEQAAIRKVAGLGLKARLIAWCRMME
DDLEAAALCDVATVNLSIPVSDLQLAAKMGRDRAWALEHIRVMVGKARAMGFDVLVGGEDSSRADPAFLADVVRACEAAG
AQRFRFADTMGIMDPFAVHDAFRALRAATSMELEIHAHDDLGLATANSLAAVRGGATHVSTTVNGLGERAGNAPLEEVVV
ALGHLYGRETGIDKRQLGRVSKLVAEASGRPVPVDKSIVGENIFTHESGIHVSGLLRDPRTYQALDPAELGRGHRLVLGK
HSGLTSVLHACREIGLEVDAAKARAMLAQVRRHASTTKTIPTADDLRRFAGTATAITGVPS
>Mature_380_residues
TKAATIVMNDTTLRDGEQTAGVAFRLDEKLDIARTLDIAGVPEIEVGIPAMGAEEQAAIRKVAGLGLKARLIAWCRMMED
DLEAAALCDVATVNLSIPVSDLQLAAKMGRDRAWALEHIRVMVGKARAMGFDVLVGGEDSSRADPAFLADVVRACEAAGA
QRFRFADTMGIMDPFAVHDAFRALRAATSMELEIHAHDDLGLATANSLAAVRGGATHVSTTVNGLGERAGNAPLEEVVVA
LGHLYGRETGIDKRQLGRVSKLVAEASGRPVPVDKSIVGENIFTHESGIHVSGLLRDPRTYQALDPAELGRGHRLVLGKH
SGLTSVLHACREIGLEVDAAKARAMLAQVRRHASTTKTIPTADDLRRFAGTATAITGVPS

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=343, Percent_Identity=34.402332361516, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1786548, Length=283, Percent_Identity=30.3886925795053, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320019, Length=347, Percent_Identity=28.8184438040346, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6320071, Length=357, Percent_Identity=28.0112044817927, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 40397; Mature: 40266

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAATIVMNDTTLRDGEQTAGVAFRLDEKLDIARTLDIAGVPEIEVGIPAMGAEEQAAI
CCCEEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCEEECCCCCCCHHHHHH
RKVAGLGLKARLIAWCRMMEDDLEAAALCDVATVNLSIPVSDLQLAAKMGRDRAWALEHI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHCCCHHHHHHHH
RVMVGKARAMGFDVLVGGEDSSRADPAFLADVVRACEAAGAQRFRFADTMGIMDPFAVHD
HHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHCCCCHHHHHH
AFRALRAATSMELEIHAHDDLGLATANSLAAVRGGATHVSTTVNGLGERAGNAPLEEVVV
HHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHH
ALGHLYGRETGIDKRQLGRVSKLVAEASGRPVPVDKSIVGENIFTHESGIHVSGLLRDPR
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEECCCCCEEEEEECCCC
TYQALDPAELGRGHRLVLGKHSGLTSVLHACREIGLEVDAAKARAMLAQVRRHASTTKTI
CCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
PTADDLRRFAGTATAITGVPS
CCHHHHHHHHCCHHHCCCCCC
>Mature Secondary Structure 
TKAATIVMNDTTLRDGEQTAGVAFRLDEKLDIARTLDIAGVPEIEVGIPAMGAEEQAAI
CCEEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCEEECCCCCCCHHHHHH
RKVAGLGLKARLIAWCRMMEDDLEAAALCDVATVNLSIPVSDLQLAAKMGRDRAWALEHI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHCCCHHHHHHHH
RVMVGKARAMGFDVLVGGEDSSRADPAFLADVVRACEAAGAQRFRFADTMGIMDPFAVHD
HHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHCCCCHHHHHH
AFRALRAATSMELEIHAHDDLGLATANSLAAVRGGATHVSTTVNGLGERAGNAPLEEVVV
HHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHH
ALGHLYGRETGIDKRQLGRVSKLVAEASGRPVPVDKSIVGENIFTHESGIHVSGLLRDPR
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEECCCCCEEEEEECCCC
TYQALDPAELGRGHRLVLGKHSGLTSVLHACREIGLEVDAAKARAMLAQVRRHASTTKTI
CCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
PTADDLRRFAGTATAITGVPS
CCHHHHHHHHCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672 [H]