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The map label for this gene is ntcA [H]

Identifier: 83310657

GI number: 83310657

Start: 1687880

End: 1688587

Strand: Reverse

Name: ntcA [H]

Synonym: amb1558

Alternate gene names: 83310657

Gene position: 1688587-1687880 (Counterclockwise)

Preceding gene: 83310658

Following gene: 83310655

Centisome position: 34.0

GC content: 67.37

Gene sequence:

>708_bases
ATGGGCGCGTCGAAGACTTGCTGCGGATGTGAGGCTTGGTCTTCGCCTCGACGCGAGCCGATGCGCGACGTCCTGGGAAA
GCGCTGCCCCTTCCCCACCCTGTCGTCCCGGTTCTCCCTGGCCAAGGGCAAGAACCTCTATCACCAGGGCGACCTGGTGG
GAGGCGCCTATAGCCTGGCCACCGGCATGGTGGCGCTGGAGCGGGTGGACGAGGACGGCGATCTGGTCATCTTGAAAATT
CTCCATCCCGGCTCGTTCTTTCCCTGCGCCAACATGCTGGGCGGCAACTTTCATGAAAGCGCGGCGCGGGCTCTGACCGA
TATCTCCGGCTGCTTCGTGCCGGCCGAGCGGCTGACTACCGCCTTGCACGACGACCCTTCGGTCGGGCTGGCCTTGCTGA
AGCTCAGCGCGGCGGAAATCCGCGAGAACGAGGACGCCATCTTCCGCCTGTGCAGCGCCGATCTGCCCGACCGGGTGCTG
TCCACTCTGAGCGCCCTGGCGGCCGAGACCGGAGGGCACGAGTCCAATGGCGACCTGTCGCTGGACCTGCCCATGTCGTG
GCGCGATCTAGCGGCCATGGTGGGCACCGGGCCGGAGGTGATCTCGCGCCTGCTGCGCCGCCTGGCCGATGCCGGGCATC
TCTCGTTCCAGGGGCGGCACGTCACCTTGCATGCCCAGGGCTCCCGCCCAGCCGCCCCCTATTCCTGA

Upstream 100 bases:

>100_bases
ATGATTTTAGAACTATTCCTTATCGAATATTTTTTTCTTGATTTGAAACGCCAATCGTCATGACATCTGCCACGCCATCA
TCCTGAGGAGGGAGAGCAGA

Downstream 100 bases:

>100_bases
TCCTTGGCGAACGCCCTGACGACCGGCTGCGAGGCGGCATCCGGCGAAGGGATGTTGCATTCCCGTTGCGGCCTGGTTAG
TTTATTTTACCATTGATCAG

Product: cAMP-binding protein - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinase

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MGASKTCCGCEAWSSPRREPMRDVLGKRCPFPTLSSRFSLAKGKNLYHQGDLVGGAYSLATGMVALERVDEDGDLVILKI
LHPGSFFPCANMLGGNFHESAARALTDISGCFVPAERLTTALHDDPSVGLALLKLSAAEIRENEDAIFRLCSADLPDRVL
STLSALAAETGGHESNGDLSLDLPMSWRDLAAMVGTGPEVISRLLRRLADAGHLSFQGRHVTLHAQGSRPAAPYS

Sequences:

>Translated_235_residues
MGASKTCCGCEAWSSPRREPMRDVLGKRCPFPTLSSRFSLAKGKNLYHQGDLVGGAYSLATGMVALERVDEDGDLVILKI
LHPGSFFPCANMLGGNFHESAARALTDISGCFVPAERLTTALHDDPSVGLALLKLSAAEIRENEDAIFRLCSADLPDRVL
STLSALAAETGGHESNGDLSLDLPMSWRDLAAMVGTGPEVISRLLRRLADAGHLSFQGRHVTLHAQGSRPAAPYS
>Mature_234_residues
GASKTCCGCEAWSSPRREPMRDVLGKRCPFPTLSSRFSLAKGKNLYHQGDLVGGAYSLATGMVALERVDEDGDLVILKIL
HPGSFFPCANMLGGNFHESAARALTDISGCFVPAERLTTALHDDPSVGLALLKLSAAEIRENEDAIFRLCSADLPDRVLS
TLSALAAETGGHESNGDLSLDLPMSWRDLAAMVGTGPEVISRLLRRLADAGHLSFQGRHVTLHAQGSRPAAPYS

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3' [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=188, Percent_Identity=26.063829787234, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25073; Mature: 24942

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGASKTCCGCEAWSSPRREPMRDVLGKRCPFPTLSSRFSLAKGKNLYHQGDLVGGAYSLA
CCCCCCCCCCHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHH
TGMVALERVDEDGDLVILKILHPGSFFPCANMLGGNFHESAARALTDISGCFVPAERLTT
HHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHH
ALHDDPSVGLALLKLSAAEIRENEDAIFRLCSADLPDRVLSTLSALAAETGGHESNGDLS
HHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEE
LDLPMSWRDLAAMVGTGPEVISRLLRRLADAGHLSFQGRHVTLHAQGSRPAAPYS
EECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEECCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
GASKTCCGCEAWSSPRREPMRDVLGKRCPFPTLSSRFSLAKGKNLYHQGDLVGGAYSLA
CCCCCCCCCHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHH
TGMVALERVDEDGDLVILKILHPGSFFPCANMLGGNFHESAARALTDISGCFVPAERLTT
HHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHH
ALHDDPSVGLALLKLSAAEIRENEDAIFRLCSADLPDRVLSTLSALAAETGGHESNGDLS
HHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEE
LDLPMSWRDLAAMVGTGPEVISRLLRRLADAGHLSFQGRHVTLHAQGSRPAAPYS
EECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEECCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927 [H]