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The map label for this gene is rfbE [H]

Identifier: 83310169

GI number: 83310169

Start: 1133263

End: 1133997

Strand: Reverse

Name: rfbE [H]

Synonym: amb1070

Alternate gene names: 83310169

Gene position: 1133997-1133263 (Counterclockwise)

Preceding gene: 83310170

Following gene: 83310168

Centisome position: 22.83

GC content: 63.67

Gene sequence:

>735_bases
ATGATGGCCAGGCTTGAACTGCAGGACGTGACCGTCACCTATCGGCTCTACGATGCCGAGGGCCGCTCGTTCACCAGATC
ACTGCTGAAGGGAGCCGGTGGCTCCGGCGCCATGACCGTCTCGGCGCTGGACAGCATGAGCCTGAGCATCGAGGCCGGCA
GCCGCATCGCGGTCCTCGGCTCGAACAGCTCGGGCAAGACCACCCTATTGCGGGTCATGGCCGGTCTGCTGCCCCCAAAA
TCGGGAACCATTCTTCGAGACGGCAAGCCCGGCGCCGTGTTCGGCATGGGTTTTGGCGTCGATCCGGAAGCAAGTCTGGG
CGACCTGGCTCTGGCGCAGGGATTGCTGATGGGCCTGACGCTGGCCGAGGCTTCCGCCAAGGTCCGTCACATCCTCGAGT
TCTCCGGCGTGAGCGACCATGGGATGTCCAAGGCCCGGATCACACCGCCGGGAATCCTCGCTCGGCTCGGTATAGCCTCC
GCCCTGTGCCTGGATGCGGACATCATCTTGCTCGATGAAGTCCTCGAACTGGTGGACCCGGTATTTCACCAGCGGGTCCT
GGATGAAATGGAGCAACGGTTACGGCGAGGAGCGATCCTGATCGTCGTCGACCGCTCACGCGATCTGCTGTCACGCTTCT
GCTCGGAGGCCCTTCTGCTCCGCGACGGGCGGATAGCCATGCGGGCACCGCTGGCGGAGGCTTTTCACAATTCCGCCGTT
AGCCAGACCTTCTGA

Upstream 100 bases:

>100_bases
CCGTCAATTGGTGGGTCAGTCTTGCCATGGCCCTCACCGGGGGATTAGCCTCGGTCGGACTATACCGCTGGTCACGTCCC
CGCTGGCCATACTGGATCTG

Downstream 100 bases:

>100_bases
TTGGTTGGATAAGCTATTCGTGCTAATTGCTGTCGCCTACGAGGAAACTGGGGATCGGTAACGCTCAATGGGCTTGCGTC
AAGGGATGAACATCGTTGTC

Product: ABC-type polysaccharide/polyol phosphate transport system

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MMARLELQDVTVTYRLYDAEGRSFTRSLLKGAGGSGAMTVSALDSMSLSIEAGSRIAVLGSNSSGKTTLLRVMAGLLPPK
SGTILRDGKPGAVFGMGFGVDPEASLGDLALAQGLLMGLTLAEASAKVRHILEFSGVSDHGMSKARITPPGILARLGIAS
ALCLDADIILLDEVLELVDPVFHQRVLDEMEQRLRRGAILIVVDRSRDLLSRFCSEALLLRDGRIAMRAPLAEAFHNSAV
SQTF

Sequences:

>Translated_244_residues
MMARLELQDVTVTYRLYDAEGRSFTRSLLKGAGGSGAMTVSALDSMSLSIEAGSRIAVLGSNSSGKTTLLRVMAGLLPPK
SGTILRDGKPGAVFGMGFGVDPEASLGDLALAQGLLMGLTLAEASAKVRHILEFSGVSDHGMSKARITPPGILARLGIAS
ALCLDADIILLDEVLELVDPVFHQRVLDEMEQRLRRGAILIVVDRSRDLLSRFCSEALLLRDGRIAMRAPLAEAFHNSAV
SQTF
>Mature_244_residues
MMARLELQDVTVTYRLYDAEGRSFTRSLLKGAGGSGAMTVSALDSMSLSIEAGSRIAVLGSNSSGKTTLLRVMAGLLPPK
SGTILRDGKPGAVFGMGFGVDPEASLGDLALAQGLLMGLTLAEASAKVRHILEFSGVSDHGMSKARITPPGILARLGIAS
ALCLDADIILLDEVLELVDPVFHQRVLDEMEQRLRRGAILIVVDRSRDLLSRFCSEALLLRDGRIAMRAPLAEAFHNSAV
SQTF

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbD [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI105990541, Length=199, Percent_Identity=29.6482412060301, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787547, Length=233, Percent_Identity=26.1802575107296, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786398, Length=229, Percent_Identity=24.4541484716157, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1787112, Length=207, Percent_Identity=30.9178743961353, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789891, Length=211, Percent_Identity=26.5402843601896, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786872, Length=198, Percent_Identity=28.7878787878788, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786253, Length=198, Percent_Identity=28.2828282828283, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789032, Length=260, Percent_Identity=21.5384615384615, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788225, Length=207, Percent_Identity=26.5700483091787, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25950; Mature: 25950

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMARLELQDVTVTYRLYDAEGRSFTRSLLKGAGGSGAMTVSALDSMSLSIEAGSRIAVLG
CCCEEEEEEEEEEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCEEEEEE
SNSSGKTTLLRVMAGLLPPKSGTILRDGKPGAVFGMGFGVDPEASLGDLALAQGLLMGLT
CCCCCHHHHHHHHHHHCCCCCCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHH
LAEASAKVRHILEFSGVSDHGMSKARITPPGILARLGIASALCLDADIILLDEVLELVDP
HHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VFHQRVLDEMEQRLRRGAILIVVDRSRDLLSRFCSEALLLRDGRIAMRAPLAEAFHNSAV
HHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SQTF
HCCC
>Mature Secondary Structure
MMARLELQDVTVTYRLYDAEGRSFTRSLLKGAGGSGAMTVSALDSMSLSIEAGSRIAVLG
CCCEEEEEEEEEEEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCEEEEEE
SNSSGKTTLLRVMAGLLPPKSGTILRDGKPGAVFGMGFGVDPEASLGDLALAQGLLMGLT
CCCCCHHHHHHHHHHHCCCCCCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHH
LAEASAKVRHILEFSGVSDHGMSKARITPPGILARLGIASALCLDADIILLDEVLELVDP
HHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VFHQRVLDEMEQRLRRGAILIVVDRSRDLLSRFCSEALLLRDGRIAMRAPLAEAFHNSAV
HHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SQTF
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]