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The map label for this gene is ytcB [H]

Identifier: 83310168

GI number: 83310168

Start: 1132188

End: 1133195

Strand: Reverse

Name: ytcB [H]

Synonym: amb1069

Alternate gene names: 83310168

Gene position: 1133195-1132188 (Counterclockwise)

Preceding gene: 83310169

Following gene: 83310167

Centisome position: 22.81

GC content: 63.0

Gene sequence:

>1008_bases
ATGGGCTTGCGTCAAGGGATGAACATCGTTGTCACTGGTGGAGCTGGCTTCATCGGTTCAACCCTGGTCCGACGCCTGCT
GGACCTGGGATGTTCCGTTTCCGTGATTGACGACCTGTCCGGCGGACGGGAGGAAAACCTACCCAATCATCCTGGGGTTA
CCCTGCACCGCCTGCGGATTGGCACCGACACGGCCGATCAGGTCGAGGCCGTGGTGGCGGACGCCGACATGGTTTATCAC
TTGGCCAGCCCCATCGGTGTCGCCCTGGCGCACCAGGCCCGTTACGAGGTGGTCGAGAATATCCTGAGTTCGGGAATTGC
CGTCGTCAGAGCATGTAAAGCGCATCGGCGCCCGCTGGTACTCACGTCAAGTTCGGAAATTTACGGCGGCGGTCTGCCGC
GGCCCTTGCGCGAGGCAGACCCCTCCTGTCTCGACATCGCCCCCCGCTGGGGCTATGCCAGTGCCAAGATGGCGTTAGAG
CAGATGGGCGCCGGCCTTTGCCTGGAACATGGGGTGCCTGTCTGGCTGATCCGCCCCTTCAACATCGCGGGTATTCGCCA
GCGGTCGGAGACGGGATTGGTGGTCGCCTCCTTCGTGGCTGCCGCCATGCAGAACCGCCCCCTTGATGTTCACGGCGATG
GTTCGCAGCTCCGGAGCTTTCTGCACGTGGAAGATGCCGCCGATGCGCTGATGGCTGTGACGTCGGCCGCATCCCTGGCC
GGGCGCCCAATCAATATCGGCAGCGAATCGCCCATCACGATTGGCGCCCTCGCCCGGCTGGTTCTGGATATTGTCGGAAG
CGCCTCTCCTATCATCCAGACGCCGTACGACGACATGTTCGATGGGTTATTTACCCACGCAACATCGCGAATCCCTGACA
TCGGATTGATTAAGGGGGCCACGGACTGGCGCCCCCACCGGTCCCTCGATGACGCCATCCGAGATTGCTGGCGCGGCATG
GCCGACGACACCCCCCGGCTACCCCCGACGACCGTCTCCCGGTTGTAA

Upstream 100 bases:

>100_bases
TTTCACAATTCCGCCGTTAGCCAGACCTTCTGATTGGTTGGATAAGCTATTCGTGCTAATTGCTGTCGCCTACGAGGAAA
CTGGGGATCGGTAACGCTCA

Downstream 100 bases:

>100_bases
CTTGGCAGCCGCCGGCTTCCTCCCCATCCTCATCGCCATACCGGCGGCGGCGGCAGGCACTTGGCTGCTGGCCAAGCTGG
CCCACCGCCTGGGAGCCGTG

Product: nucleoside-diphosphate-sugar epimerase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MGLRQGMNIVVTGGAGFIGSTLVRRLLDLGCSVSVIDDLSGGREENLPNHPGVTLHRLRIGTDTADQVEAVVADADMVYH
LASPIGVALAHQARYEVVENILSSGIAVVRACKAHRRPLVLTSSSEIYGGGLPRPLREADPSCLDIAPRWGYASAKMALE
QMGAGLCLEHGVPVWLIRPFNIAGIRQRSETGLVVASFVAAAMQNRPLDVHGDGSQLRSFLHVEDAADALMAVTSAASLA
GRPINIGSESPITIGALARLVLDIVGSASPIIQTPYDDMFDGLFTHATSRIPDIGLIKGATDWRPHRSLDDAIRDCWRGM
ADDTPRLPPTTVSRL

Sequences:

>Translated_335_residues
MGLRQGMNIVVTGGAGFIGSTLVRRLLDLGCSVSVIDDLSGGREENLPNHPGVTLHRLRIGTDTADQVEAVVADADMVYH
LASPIGVALAHQARYEVVENILSSGIAVVRACKAHRRPLVLTSSSEIYGGGLPRPLREADPSCLDIAPRWGYASAKMALE
QMGAGLCLEHGVPVWLIRPFNIAGIRQRSETGLVVASFVAAAMQNRPLDVHGDGSQLRSFLHVEDAADALMAVTSAASLA
GRPINIGSESPITIGALARLVLDIVGSASPIIQTPYDDMFDGLFTHATSRIPDIGLIKGATDWRPHRSLDDAIRDCWRGM
ADDTPRLPPTTVSRL
>Mature_334_residues
GLRQGMNIVVTGGAGFIGSTLVRRLLDLGCSVSVIDDLSGGREENLPNHPGVTLHRLRIGTDTADQVEAVVADADMVYHL
ASPIGVALAHQARYEVVENILSSGIAVVRACKAHRRPLVLTSSSEIYGGGLPRPLREADPSCLDIAPRWGYASAKMALEQ
MGAGLCLEHGVPVWLIRPFNIAGIRQRSETGLVVASFVAAAMQNRPLDVHGDGSQLRSFLHVEDAADALMAVTSAASLAG
RPINIGSESPITIGALARLVLDIVGSASPIIQTPYDDMFDGLFTHATSRIPDIGLIKGATDWRPHRSLDDAIRDCWRGMA
DDTPRLPPTTVSRL

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=314, Percent_Identity=31.8471337579618, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI7657641, Length=276, Percent_Identity=25, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1788589, Length=277, Percent_Identity=25.6317689530686, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17539532, Length=333, Percent_Identity=30.3303303303303, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI115532424, Length=326, Percent_Identity=25.4601226993865, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356223, Length=318, Percent_Identity=29.2452830188679, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 35699; Mature: 35568

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLRQGMNIVVTGGAGFIGSTLVRRLLDLGCSVSVIDDLSGGREENLPNHPGVTLHRLRI
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCCCCCEEEEEEE
GTDTADQVEAVVADADMVYHLASPIGVALAHQARYEVVENILSSGIAVVRACKAHRRPLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTSSSEIYGGGLPRPLREADPSCLDIAPRWGYASAKMALEQMGAGLCLEHGVPVWLIRPF
EECCCCCCCCCCCCCHHHCCCCCEECCCCCCCHHHHHHHHHHCCCEEHHCCCCEEEEECC
NIAGIRQRSETGLVVASFVAAAMQNRPLDVHGDGSQLRSFLHVEDAADALMAVTSAASLA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRPINIGSESPITIGALARLVLDIVGSASPIIQTPYDDMFDGLFTHATSRIPDIGLIKGA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCCEEECCC
TDWRPHRSLDDAIRDCWRGMADDTPRLPPTTVSRL
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
GLRQGMNIVVTGGAGFIGSTLVRRLLDLGCSVSVIDDLSGGREENLPNHPGVTLHRLRI
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCCCCCEEEEEEE
GTDTADQVEAVVADADMVYHLASPIGVALAHQARYEVVENILSSGIAVVRACKAHRRPLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTSSSEIYGGGLPRPLREADPSCLDIAPRWGYASAKMALEQMGAGLCLEHGVPVWLIRPF
EECCCCCCCCCCCCCHHHCCCCCEECCCCCCCHHHHHHHHHHCCCEEHHCCCCEEEEECC
NIAGIRQRSETGLVVASFVAAAMQNRPLDVHGDGSQLRSFLHVEDAADALMAVTSAASLA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRPINIGSESPITIGALARLVLDIVGSASPIIQTPYDDMFDGLFTHATSRIPDIGLIKGA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCCEEECCC
TDWRPHRSLDDAIRDCWRGMADDTPRLPPTTVSRL
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]