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The map label for this gene is gcvA [H]

Identifier: 83309925

GI number: 83309925

Start: 887667

End: 888557

Strand: Reverse

Name: gcvA [H]

Synonym: amb0826

Alternate gene names: 83309925

Gene position: 888557-887667 (Counterclockwise)

Preceding gene: 83309928

Following gene: 83309924

Centisome position: 17.89

GC content: 73.63

Gene sequence:

>891_bases
ATGCGCCGTCGCCTCCCGCCCCTCCGGGCCCTGCATGCCTTCGAGGCCGCCGCCCGCCATCTGTCCTTCGCGCGGGCCGC
CGAGGAGCTGGGCGTCACCCCGGCGGCCATCAGCCAGCAGATCAAGCAATTGGAGGACATCCTGGGCGTCGGCCTGTTCC
ATCGCGGAACGCGCCTGTCGCTGACCCGGGCCGCCGCCCTGGTCTCGGGCGAGGTCAGCGACGCCTTCGACCGCCTGGCC
TCCGCCGTGGGACGGCTGGCCCCCGACGGCCCGGCCCGGCCGCTGGTGGTGTCGGCGCCGCCGGCCTTCGCGGCGCGCTG
GCTGGTCCCCCGCCTCACCCGCTTCCAGGACGCCCATCCCGGCATTGACCTGCACCTGTCCGCCACGTTGCGGCTGGTGG
ATCTGGACCGCGAGGGCGTGGACCTGGCCATCCGCTACGGACCGGGACGCTATCCCGGGCTGCTGGTGGAACGGCTACGC
CTGGAAGAGGTGGTGGCGGTGGCGACGCCGCGTCTGGCCGCCGGCCTGGGCGAGGTGTCCGATCTCGCCCGCGCCACCTT
GCTGGTCAATGAAGGCATCGGCTGGGACCCGTCCTTCCCCACCTGGCCGGCCTGGCTGTCCGAGATGGGCGCCAGCCCGG
CCGAGACGCTGCGCCTGCGGCCCTTCGGCGACGCCTCCCTGGTCCTGGAGGCCGCCCTGGCCGGACTGGGCGTCGCCCTG
GTCTGGCGCACCCTGGTTTGTGACGAACTGGCCGGCGGCCGACTGGTGGCCCTGTTCCCCGGACGCCCCCTGGCCAGCGC
CTATCATCTGGCCTGCCTGCCCGAGCGCGCCGAATTCCCGCCCATCCGGGCATTCCGGGACTGGATCAAGGCGGAACTCG
TCACCACGTGA

Upstream 100 bases:

>100_bases
ATCTCCATTGAGATAGACTAATCATGGTCGCAACGATACGCTTTGGAACTTCTTGGCTTCAAACGAGCTTTTCTCGCCCT
CGGGTTAAGGAAATCTAATC

Downstream 100 bases:

>100_bases
TGGACTTATCCCGTTCGTAGCCTTGCCATCCCCCGATGGGATGTTAGGCTGGAGGTCGGGTCAATCCCTGAGCAGGAGTC
GGCCGATGAAAGGCAAGAAG

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRRRLPPLRALHAFEAAARHLSFARAAEELGVTPAAISQQIKQLEDILGVGLFHRGTRLSLTRAAALVSGEVSDAFDRLA
SAVGRLAPDGPARPLVVSAPPAFAARWLVPRLTRFQDAHPGIDLHLSATLRLVDLDREGVDLAIRYGPGRYPGLLVERLR
LEEVVAVATPRLAAGLGEVSDLARATLLVNEGIGWDPSFPTWPAWLSEMGASPAETLRLRPFGDASLVLEAALAGLGVAL
VWRTLVCDELAGGRLVALFPGRPLASAYHLACLPERAEFPPIRAFRDWIKAELVTT

Sequences:

>Translated_296_residues
MRRRLPPLRALHAFEAAARHLSFARAAEELGVTPAAISQQIKQLEDILGVGLFHRGTRLSLTRAAALVSGEVSDAFDRLA
SAVGRLAPDGPARPLVVSAPPAFAARWLVPRLTRFQDAHPGIDLHLSATLRLVDLDREGVDLAIRYGPGRYPGLLVERLR
LEEVVAVATPRLAAGLGEVSDLARATLLVNEGIGWDPSFPTWPAWLSEMGASPAETLRLRPFGDASLVLEAALAGLGVAL
VWRTLVCDELAGGRLVALFPGRPLASAYHLACLPERAEFPPIRAFRDWIKAELVTT
>Mature_296_residues
MRRRLPPLRALHAFEAAARHLSFARAAEELGVTPAAISQQIKQLEDILGVGLFHRGTRLSLTRAAALVSGEVSDAFDRLA
SAVGRLAPDGPARPLVVSAPPAFAARWLVPRLTRFQDAHPGIDLHLSATLRLVDLDREGVDLAIRYGPGRYPGLLVERLR
LEEVVAVATPRLAAGLGEVSDLARATLLVNEGIGWDPSFPTWPAWLSEMGASPAETLRLRPFGDASLVLEAALAGLGVAL
VWRTLVCDELAGGRLVALFPGRPLASAYHLACLPERAEFPPIRAFRDWIKAELVTT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=42.0875420875421, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=33.6769759450172, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=32.996632996633, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=27.1812080536913, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081978, Length=270, Percent_Identity=31.4814814814815, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=27.1477663230241, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787128, Length=300, Percent_Identity=28, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=153, Percent_Identity=34.640522875817, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31919; Mature: 31919

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRLPPLRALHAFEAAARHLSFARAAEELGVTPAAISQQIKQLEDILGVGLFHRGTRLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
LTRAAALVSGEVSDAFDRLASAVGRLAPDGPARPLVVSAPPAFAARWLVPRLTRFQDAHP
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCC
GIDLHLSATLRLVDLDREGVDLAIRYGPGRYPGLLVERLRLEEVVAVATPRLAAGLGEVS
CEEEEEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DLARATLLVNEGIGWDPSFPTWPAWLSEMGASPAETLRLRPFGDASLVLEAALAGLGVAL
HHHHHHHHEECCCCCCCCCCCHHHHHHHHCCCHHHHEEECCCCCHHHHHHHHHHHHHHHH
VWRTLVCDELAGGRLVALFPGRPLASAYHLACLPERAEFPPIRAFRDWIKAELVTT
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHEECCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRRLPPLRALHAFEAAARHLSFARAAEELGVTPAAISQQIKQLEDILGVGLFHRGTRLS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
LTRAAALVSGEVSDAFDRLASAVGRLAPDGPARPLVVSAPPAFAARWLVPRLTRFQDAHP
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCC
GIDLHLSATLRLVDLDREGVDLAIRYGPGRYPGLLVERLRLEEVVAVATPRLAAGLGEVS
CEEEEEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DLARATLLVNEGIGWDPSFPTWPAWLSEMGASPAETLRLRPFGDASLVLEAALAGLGVAL
HHHHHHHHEECCCCCCCCCCCHHHHHHHHCCCHHHHEEECCCCCHHHHHHHHHHHHHHHH
VWRTLVCDELAGGRLVALFPGRPLASAYHLACLPERAEFPPIRAFRDWIKAELVTT
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHEECCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]