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The map label for this gene is mvaB [H]

Identifier: 83309772

GI number: 83309772

Start: 722387

End: 723280

Strand: Reverse

Name: mvaB [H]

Synonym: amb0673

Alternate gene names: 83309772

Gene position: 723280-722387 (Counterclockwise)

Preceding gene: 83309773

Following gene: 83309771

Centisome position: 14.56

GC content: 69.46

Gene sequence:

>894_bases
ATGCGTCATCCCTCGCGCGTCAAAATCGTCGAGGTCGGTCCCCGCGACGGCCTGCAGAACGAGGCCAGGCCGGTCGCCGT
CGAGATCAGGGTCGGGCTGATCAATCGCCTGACCGGGACCGGGCTTACCGCCATCGAATCGGGCAGCTTCGTCAGCCCCA
AATGGGTGCCGCAGATGGCCGCCACCGCCGAGGTGATGGCCGCCATCACGCGGCGGCCGGGCGTGGCCTATCCCGTGCTT
ACCCCCAACCTCCAGGGGCTGGAGGCGGCGCTGGCCGCCAAGGTCGAGGAGGTGGCGGTGTTCGCCGCGGCGTCGGAGAC
CTTCTCGCAAAAGAACATCAACTGCTCCATCGCCGAAAGCCTGGAGCGCTTCGCCCCGCTGGCCGCCCGGGCCAAGGCCG
AGGGGTTGCGGGTGCGGGGCTATGTCTCCTGCGTGCTCGGCTGCCCCTATGAGGGCGCGGTCGAGCCCTCGGCGGTGGCC
GGAGTGGCGGAAAAGCTGGTGGCCATGGGCTGCTACGAGATCTCGCTGGGCGATACGGTGGGCGTCGGCACCCCCGCCAA
GGCCCAGGCCATGATCGACGCGGTCACCGCTCGGCTGCCCGTCGAGATGCTGGCCGCCCATTTCCACGACACCTATGGCC
AGGCCCTGGCCAATCTGCTGGCGGTGATGGAGCGCGGCGTGGCGGTGATGGATTCCTCCGTCGCCGGGCTGGGCGGCTGC
CCCTATGCCAAGGGCGCCGCCGGCAACGTGGCCAGCGAGGATCTGGTCTACATGCTGAACGGCATGGGCATTCACACCGG
CATCGACCTGGACAGGCTGATCGAGGCCGGAACCTTCATCTGCGAGGCGCTTGGCCGCGCCACGGGCTCGAAGGTTGCCC
GCGCCCGCGCTTAG

Upstream 100 bases:

>100_bases
CAAACCCCCTTTTGATTCATGAATGAAAGGGAGGTTTGGAGGTCTCCTCCAGGCGGGGAACGGGGCGGCAGCCCCGAATG
GACGGGAAAGGTTCGAGATC

Downstream 100 bases:

>100_bases
CTTGGCTTGACCAACGACCTTGGCGATAAGTATAGCCGTGTGCTACCATCGAACCCGCGAAATTCACAAATGCGGACACG
AGCTCCATGTCCACGGATTT

Product: isopropylmalate/homocitrate/citramalate synthase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRHPSRVKIVEVGPRDGLQNEARPVAVEIRVGLINRLTGTGLTAIESGSFVSPKWVPQMAATAEVMAAITRRPGVAYPVL
TPNLQGLEAALAAKVEEVAVFAAASETFSQKNINCSIAESLERFAPLAARAKAEGLRVRGYVSCVLGCPYEGAVEPSAVA
GVAEKLVAMGCYEISLGDTVGVGTPAKAQAMIDAVTARLPVEMLAAHFHDTYGQALANLLAVMERGVAVMDSSVAGLGGC
PYAKGAAGNVASEDLVYMLNGMGIHTGIDLDRLIEAGTFICEALGRATGSKVARARA

Sequences:

>Translated_297_residues
MRHPSRVKIVEVGPRDGLQNEARPVAVEIRVGLINRLTGTGLTAIESGSFVSPKWVPQMAATAEVMAAITRRPGVAYPVL
TPNLQGLEAALAAKVEEVAVFAAASETFSQKNINCSIAESLERFAPLAARAKAEGLRVRGYVSCVLGCPYEGAVEPSAVA
GVAEKLVAMGCYEISLGDTVGVGTPAKAQAMIDAVTARLPVEMLAAHFHDTYGQALANLLAVMERGVAVMDSSVAGLGGC
PYAKGAAGNVASEDLVYMLNGMGIHTGIDLDRLIEAGTFICEALGRATGSKVARARA
>Mature_297_residues
MRHPSRVKIVEVGPRDGLQNEARPVAVEIRVGLINRLTGTGLTAIESGSFVSPKWVPQMAATAEVMAAITRRPGVAYPVL
TPNLQGLEAALAAKVEEVAVFAAASETFSQKNINCSIAESLERFAPLAARAKAEGLRVRGYVSCVLGCPYEGAVEPSAVA
GVAEKLVAMGCYEISLGDTVGVGTPAKAQAMIDAVTARLPVEMLAAHFHDTYGQALANLLAVMERGVAVMDSSVAGLGGC
PYAKGAAGNVASEDLVYMLNGMGIHTGIDLDRLIEAGTFICEALGRATGSKVARARA

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=292, Percent_Identity=61.3013698630137, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI109150427, Length=292, Percent_Identity=58.9041095890411, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI109150422, Length=292, Percent_Identity=58.9041095890411, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI260654708, Length=136, Percent_Identity=64.7058823529412, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17510563, Length=292, Percent_Identity=54.1095890410959, Blast_Score=326, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24582381, Length=291, Percent_Identity=60.4810996563574, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 30807; Mature: 30807

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01062 HMG_COA_LYASE ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHPSRVKIVEVGPRDGLQNEARPVAVEIRVGLINRLTGTGLTAIESGSFVSPKWVPQMA
CCCCCEEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHCCCCCEEECCCCCCCCCHHHHHH
ATAEVMAAITRRPGVAYPVLTPNLQGLEAALAAKVEEVAVFAAASETFSQKNINCSIAES
HHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LERFAPLAARAKAEGLRVRGYVSCVLGCPYEGAVEPSAVAGVAEKLVAMGCYEISLGDTV
HHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCC
GVGTPAKAQAMIDAVTARLPVEMLAAHFHDTYGQALANLLAVMERGVAVMDSSVAGLGGC
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCC
PYAKGAAGNVASEDLVYMLNGMGIHTGIDLDRLIEAGTFICEALGRATGSKVARARA
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MRHPSRVKIVEVGPRDGLQNEARPVAVEIRVGLINRLTGTGLTAIESGSFVSPKWVPQMA
CCCCCEEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHCCCCCEEECCCCCCCCCHHHHHH
ATAEVMAAITRRPGVAYPVLTPNLQGLEAALAAKVEEVAVFAAASETFSQKNINCSIAES
HHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LERFAPLAARAKAEGLRVRGYVSCVLGCPYEGAVEPSAVAGVAEKLVAMGCYEISLGDTV
HHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCC
GVGTPAKAQAMIDAVTARLPVEMLAAHFHDTYGQALANLLAVMERGVAVMDSSVAGLGGC
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCC
PYAKGAAGNVASEDLVYMLNGMGIHTGIDLDRLIEAGTFICEALGRATGSKVARARA
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]