Click here to switch to the map view.

The map label for this gene is aatA [H]

Identifier: 83309654

GI number: 83309654

Start: 603257

End: 604459

Strand: Reverse

Name: aatA [H]

Synonym: amb0555

Alternate gene names: 83309654

Gene position: 604459-603257 (Counterclockwise)

Preceding gene: 83309655

Following gene: 83309652

Centisome position: 12.17

GC content: 66.08

Gene sequence:

>1203_bases
ATGCCGTTCATCGCCGACAGGCTTTCCGCCATCAAGCCGTCCCCGACCATTGCGGTGACCCAGAAGGCCGCCGAGTTGAA
GGCCGCCGGCCGCGACGTCATCGGCCTGGGCGCGGGCGAGCCCGATTTCGACACGCCGGACAACATCAAGGCCGCCGCCA
AGGCCGCCATCGACAAGGGTCTGACCAAGTACGGCCCGCCGGCCGGCACCGTGGACCTGCGCCAGGCCATCGCCGCCAAG
TTCAAGCGCGAGAACGGCCTGGACTACACCGCCGATCAGGTGACCGTGGGCGTCGGCGGCAAGGGCGTGATCTTCAACGC
CTTCATGGCCACCATCAATCCCGGCGACGAGGTGATCGTGCCCGCCCCCTATTGGGTGAGCTATCCCGACATCGCCCTGA
TGTTCGGCGGCAAGCCGGTCTTCGTCCCCTGTCCCGAGACCGCCGGCTTCAAGCTGCAGCCCGCCGACCTGGAAAAGGCC
ATCACGCCGAGGACTAAGTGGCTGGTGCTGAACTCGCCGTCCAACCCGACCGGCGCCGCCTATTCCTGGGACGAGATGAA
GGCGCTGACCGACGTGCTGCTCCGGCATCCGCATGTATGGATCATGTCCGACGACATGTACGAGCACTTGGTCTATGACG
ACTTCAAGTTCTGCACCCCCGCCCAGGTGGAGCCCAAGCTGTATGACCGCACGCTGACCATGAACGGCGTGTCGAAGGCC
TATGCCATGACCGGCTGGCGCGTCGGCTATGCCGCCGGCCCGGCCGCCATCATCAAGGCCATCAACATGATCCAGTCCCA
GTCGGTGACCCACACCTCGTCCATCAGCCAGGCCGCCTCGGTGGAGGCCCTGAACGGCACCCAGGACTTCATCCCGAAGA
ACGCCGCGGTGTTCAAGCGCCGCCGCGACCTGATCGTCGGCCTGCTGAACCAGTGCCCCGGCATCACCTGCCGCACCCCG
GAAGGCGCCTTCTACGTCTATCCGTCCTGTGCCGGCGTGATCGGCAAGAAGACCCCCGACGGCAAGGTCATCGCCAACGA
CACCGACTTCGTCGGCGCGCTGCTCGAAGCCGAGGGCGTGGCGGTGGTCCAGGGTGCCGCCTTCGGCCTGGAGCCCTATT
TCCGCATCTCCTACGCCACCTCGGACGCTGCGCTGACCAAGGCCGCCGAGCGCATCAAGCGCTTCTGCGAAAGCCTGAAG
TAG

Upstream 100 bases:

>100_bases
AATTACTCGTTGCATACAACTTTGTCCCCTTGCACTCGCACGCCCGCACGCGTACCTTCGCCACCCGCGAATTCTCTCAT
CTTTTTCGAGGACCCGACCC

Downstream 100 bases:

>100_bases
GCCGCCCAAGACAAAGCCCCCCGGCCGCAGGGCCGGGGGGCTTTTTCATGCCCGGACTCAGGATGCCTGGGTGCGGATCT
CGCGCAGGAAGCCGTCGATC

Product: aspartate aminotransferase A

Products: NA

Alternate protein names: AspAT; Transaminase A [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MPFIADRLSAIKPSPTIAVTQKAAELKAAGRDVIGLGAGEPDFDTPDNIKAAAKAAIDKGLTKYGPPAGTVDLRQAIAAK
FKRENGLDYTADQVTVGVGGKGVIFNAFMATINPGDEVIVPAPYWVSYPDIALMFGGKPVFVPCPETAGFKLQPADLEKA
ITPRTKWLVLNSPSNPTGAAYSWDEMKALTDVLLRHPHVWIMSDDMYEHLVYDDFKFCTPAQVEPKLYDRTLTMNGVSKA
YAMTGWRVGYAAGPAAIIKAINMIQSQSVTHTSSISQAASVEALNGTQDFIPKNAAVFKRRRDLIVGLLNQCPGITCRTP
EGAFYVYPSCAGVIGKKTPDGKVIANDTDFVGALLEAEGVAVVQGAAFGLEPYFRISYATSDAALTKAAERIKRFCESLK

Sequences:

>Translated_400_residues
MPFIADRLSAIKPSPTIAVTQKAAELKAAGRDVIGLGAGEPDFDTPDNIKAAAKAAIDKGLTKYGPPAGTVDLRQAIAAK
FKRENGLDYTADQVTVGVGGKGVIFNAFMATINPGDEVIVPAPYWVSYPDIALMFGGKPVFVPCPETAGFKLQPADLEKA
ITPRTKWLVLNSPSNPTGAAYSWDEMKALTDVLLRHPHVWIMSDDMYEHLVYDDFKFCTPAQVEPKLYDRTLTMNGVSKA
YAMTGWRVGYAAGPAAIIKAINMIQSQSVTHTSSISQAASVEALNGTQDFIPKNAAVFKRRRDLIVGLLNQCPGITCRTP
EGAFYVYPSCAGVIGKKTPDGKVIANDTDFVGALLEAEGVAVVQGAAFGLEPYFRISYATSDAALTKAAERIKRFCESLK
>Mature_399_residues
PFIADRLSAIKPSPTIAVTQKAAELKAAGRDVIGLGAGEPDFDTPDNIKAAAKAAIDKGLTKYGPPAGTVDLRQAIAAKF
KRENGLDYTADQVTVGVGGKGVIFNAFMATINPGDEVIVPAPYWVSYPDIALMFGGKPVFVPCPETAGFKLQPADLEKAI
TPRTKWLVLNSPSNPTGAAYSWDEMKALTDVLLRHPHVWIMSDDMYEHLVYDDFKFCTPAQVEPKLYDRTLTMNGVSKAY
AMTGWRVGYAAGPAAIIKAINMIQSQSVTHTSSISQAASVEALNGTQDFIPKNAAVFKRRRDLIVGLLNQCPGITCRTPE
GAFYVYPSCAGVIGKKTPDGKVIANDTDFVGALLEAEGVAVVQGAAFGLEPYFRISYATSDAALTKAAERIKRFCESLK

Specific function: Required for symbiotic nitrogen fixation [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI95147551, Length=421, Percent_Identity=30.166270783848, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI169881279, Length=421, Percent_Identity=30.166270783848, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI56713254, Length=423, Percent_Identity=26.9503546099291, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI56713256, Length=423, Percent_Identity=26.9503546099291, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI169881281, Length=418, Percent_Identity=26.0765550239234, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI4507369, Length=381, Percent_Identity=24.6719160104987, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1788627, Length=374, Percent_Identity=30.2139037433155, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1786816, Length=386, Percent_Identity=26.9430051813472, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788722, Length=355, Percent_Identity=29.0140845070423, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1787710, Length=240, Percent_Identity=29.5833333333333, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71994472, Length=295, Percent_Identity=30.5084745762712, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71994476, Length=295, Percent_Identity=30.5084745762712, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17567663, Length=379, Percent_Identity=24.5382585751979, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17567369, Length=349, Percent_Identity=25.5014326647564, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71981209, Length=340, Percent_Identity=23.2352941176471, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6322401, Length=390, Percent_Identity=28.2051282051282, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320317, Length=362, Percent_Identity=21.8232044198895, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28573069, Length=395, Percent_Identity=25.8227848101266, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24646114, Length=395, Percent_Identity=25.8227848101266, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28573067, Length=395, Percent_Identity=25.8227848101266, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28573065, Length=395, Percent_Identity=25.8227848101266, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI18859735, Length=380, Percent_Identity=24.2105263157895, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001176
- InterPro:   IPR004839
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.6.1.1 [H]

Molecular weight: Translated: 42934; Mature: 42802

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFIADRLSAIKPSPTIAVTQKAAELKAAGRDVIGLGAGEPDFDTPDNIKAAAKAAIDKG
CCCHHHHHHHCCCCCCEEEEHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHC
LTKYGPPAGTVDLRQAIAAKFKRENGLDYTADQVTVGVGGKGVIFNAFMATINPGDEVIV
HHHCCCCCCCHHHHHHHHHHHHHCCCCCEECCEEEEECCCCCEEEEEEEEEECCCCCEEE
PAPYWVSYPDIALMFGGKPVFVPCPETAGFKLQPADLEKAITPRTKWLVLNSPSNPTGAA
CCCCEECCCCEEEEECCEEEEEECCCCCCCEECCHHHHHHCCCCEEEEEEECCCCCCCCC
YSWDEMKALTDVLLRHPHVWIMSDDMYEHLVYDDFKFCTPAQVEPKLYDRTLTMNGVSKA
CCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCCCCCCCCCHHHHCEEEEECCCHHH
YAMTGWRVGYAAGPAAIIKAINMIQSQSVTHTSSISQAASVEALNGTQDFIPKNAAVFKR
HHHCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCHHHCCCCHHHHHH
RRDLIVGLLNQCPGITCRTPEGAFYVYPSCAGVIGKKTPDGKVIANDTDFVGALLEAEGV
HHHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCE
AVVQGAAFGLEPYFRISYATSDAALTKAAERIKRFCESLK
EEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFIADRLSAIKPSPTIAVTQKAAELKAAGRDVIGLGAGEPDFDTPDNIKAAAKAAIDKG
CCHHHHHHHCCCCCCEEEEHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHC
LTKYGPPAGTVDLRQAIAAKFKRENGLDYTADQVTVGVGGKGVIFNAFMATINPGDEVIV
HHHCCCCCCCHHHHHHHHHHHHHCCCCCEECCEEEEECCCCCEEEEEEEEEECCCCCEEE
PAPYWVSYPDIALMFGGKPVFVPCPETAGFKLQPADLEKAITPRTKWLVLNSPSNPTGAA
CCCCEECCCCEEEEECCEEEEEECCCCCCCEECCHHHHHHCCCCEEEEEEECCCCCCCCC
YSWDEMKALTDVLLRHPHVWIMSDDMYEHLVYDDFKFCTPAQVEPKLYDRTLTMNGVSKA
CCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCCCCCCCCCHHHHCEEEEECCCHHH
YAMTGWRVGYAAGPAAIIKAINMIQSQSVTHTSSISQAASVEALNGTQDFIPKNAAVFKR
HHHCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCHHHCCCCHHHHHH
RRDLIVGLLNQCPGITCRTPEGAFYVYPSCAGVIGKKTPDGKVIANDTDFVGALLEAEGV
HHHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCE
AVVQGAAFGLEPYFRISYATSDAALTKAAERIKRFCESLK
EEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2019560; 8096210; 11481430 [H]