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The map label for this gene is gltD

Identifier: 83309621

GI number: 83309621

Start: 562515

End: 563930

Strand: Reverse

Name: gltD

Synonym: amb0522

Alternate gene names: 83309621

Gene position: 563930-562515 (Counterclockwise)

Preceding gene: 83309623

Following gene: 83309620

Centisome position: 11.35

GC content: 68.86

Gene sequence:

>1416_bases
ATGCCCCGCAAGATGCTGCAGTTCACCCACCTCCATCAGCGCCAGCCGGAAAAGCGCGGCGCCGCCGAGCGCCGCAAGGA
TTTCGGCGAGATCAGCACCGCCTTCGGGGCGTCCCAGGCGGGCGAGCAGGCGTCGCGCTGCGAGCAATGCGGCATTCCCT
TTTGCTCCATTCACTGCCCGCTGGGTAACAACGTGCCGGACTGGCTGATGCTGGTGGCGCAGGACCGCATGGAGGAGGCC
TACGCCGTCTCGTCGGCCACCAACACCTTCCCCGAGATCTGTGGCCGCATCTGTCCCCAGGACCGCCTGTGCGAGGGCAA
TTGCGTGCTGGAGCAATCCACCCACGGCAATGTCACCATCGGCGCGGTGGAAAAGCACATCACCGAGACCGCCTTCGCTG
AAGGATGGGTCAAGCCGGTGCTGCCCGGCGCCGAGACCGGCCGCTCGGTCGGCATCGTCGGCGGCGGCCCCGCCGGACTG
GCGGCGGCGGAATCCCTGCGCCGCAAGGGCCATGCCGTCACCGTCTACGATCGCCACGACCGCATGGGCGGGCTGCTGAT
CTATGGCATTCCCGGCTTCAAGCTGGAGAAGGACGTGGTCGAGCGCCGCATCCGCCTGCTGGCCGAGGCCGGCGTCAAGT
TCGAGACCGGCGTGGAAATCGGCAAGACCGTCTCCTTCGCCGAACTGCGCGCCCGCCACGACGCGGTGCTGATCGCCACC
GGGGTCTACAAGGCCAAGCCCCTGGGCATCAATGGCGCCGACCTGCCCGGCGTGCATGCCGCCCTGGACTACCTGATCGC
CCAGAACCGCCGCGATCTCGGCGAGACCGTGGCCGAGTTGGACGCCAAGGGCCGCAACGTGGTGGTCATCGGCGGCGGCG
ACACCGCCATGGATTGCGTGCGCACCGCCATCCGCCAGGGGGCCAAGTCGGTGAAGTGCCTCTACCGCCGCGACCGGGCC
AACATGCCCGGCTCCAAGCGCGAGGTGGCCCACGCCGAGGAAGAAGGCGTCGAATTCGTCTGGATGGCCGCCCCCGAAGC
CGTCACCGGCGCCAAGAAGGCCGAGGGCGTCCGGGCCGTGCGCATGCATCTGGGCATGCCCGATTCCTCGGGCCGTCAAA
GCCCGGTGGCCATCGAGGGATCGGGCTTCACCTTGGCCTCCGACATGGTCATCGCCGCCCTGGGCTTCGATCCCGAGGAT
ATGCCCGTCCTGTTCGGCGCCCCCGAGCTCAAGGTCTCCAAATGGGGAACCGTCGAGACCGACGCGACGCTGATGACCAG
CCTGCCCGGCGTCTTCGGAGCCGGCGACATCGTGCGCGGCGCCTCCCTGGTGGTCTGGGCCATCAAGGACGGCCGCGACG
CCGCCGAAGCCATCCACCGCTACGTCAGTGCCAAATCCGCCCAGGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
ATTTGAGACAGCAATGTTGTCTCATTTGCGCGATTCCCATTGATTGGTCACCACTTCTGACGTATTTTCGCCCGACAATT
CCCGGGCTCGAGGAACAGCC

Downstream 100 bases:

>100_bases
GAGTGCGCCCCATGTCCGATTTCGTCTCCCAATACCTCGCCAACCAGGAGATCCTGGAGGCCGCCGGTATCGACACCAGC
CCCCATGACGCCTGCGGCGT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MPRKMLQFTHLHQRQPEKRGAAERRKDFGEISTAFGASQAGEQASRCEQCGIPFCSIHCPLGNNVPDWLMLVAQDRMEEA
YAVSSATNTFPEICGRICPQDRLCEGNCVLEQSTHGNVTIGAVEKHITETAFAEGWVKPVLPGAETGRSVGIVGGGPAGL
AAAESLRRKGHAVTVYDRHDRMGGLLIYGIPGFKLEKDVVERRIRLLAEAGVKFETGVEIGKTVSFAELRARHDAVLIAT
GVYKAKPLGINGADLPGVHAALDYLIAQNRRDLGETVAELDAKGRNVVVIGGGDTAMDCVRTAIRQGAKSVKCLYRRDRA
NMPGSKREVAHAEEEGVEFVWMAAPEAVTGAKKAEGVRAVRMHLGMPDSSGRQSPVAIEGSGFTLASDMVIAALGFDPED
MPVLFGAPELKVSKWGTVETDATLMTSLPGVFGAGDIVRGASLVVWAIKDGRDAAEAIHRYVSAKSAQAAE

Sequences:

>Translated_471_residues
MPRKMLQFTHLHQRQPEKRGAAERRKDFGEISTAFGASQAGEQASRCEQCGIPFCSIHCPLGNNVPDWLMLVAQDRMEEA
YAVSSATNTFPEICGRICPQDRLCEGNCVLEQSTHGNVTIGAVEKHITETAFAEGWVKPVLPGAETGRSVGIVGGGPAGL
AAAESLRRKGHAVTVYDRHDRMGGLLIYGIPGFKLEKDVVERRIRLLAEAGVKFETGVEIGKTVSFAELRARHDAVLIAT
GVYKAKPLGINGADLPGVHAALDYLIAQNRRDLGETVAELDAKGRNVVVIGGGDTAMDCVRTAIRQGAKSVKCLYRRDRA
NMPGSKREVAHAEEEGVEFVWMAAPEAVTGAKKAEGVRAVRMHLGMPDSSGRQSPVAIEGSGFTLASDMVIAALGFDPED
MPVLFGAPELKVSKWGTVETDATLMTSLPGVFGAGDIVRGASLVVWAIKDGRDAAEAIHRYVSAKSAQAAE
>Mature_470_residues
PRKMLQFTHLHQRQPEKRGAAERRKDFGEISTAFGASQAGEQASRCEQCGIPFCSIHCPLGNNVPDWLMLVAQDRMEEAY
AVSSATNTFPEICGRICPQDRLCEGNCVLEQSTHGNVTIGAVEKHITETAFAEGWVKPVLPGAETGRSVGIVGGGPAGLA
AAESLRRKGHAVTVYDRHDRMGGLLIYGIPGFKLEKDVVERRIRLLAEAGVKFETGVEIGKTVSFAELRARHDAVLIATG
VYKAKPLGINGADLPGVHAALDYLIAQNRRDLGETVAELDAKGRNVVVIGGGDTAMDCVRTAIRQGAKSVKCLYRRDRAN
MPGSKREVAHAEEEGVEFVWMAAPEAVTGAKKAEGVRAVRMHLGMPDSSGRQSPVAIEGSGFTLASDMVIAALGFDPEDM
PVLFGAPELKVSKWGTVETDATLMTSLPGVFGAGDIVRGASLVVWAIKDGRDAAEAIHRYVSAKSAQAAE

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=484, Percent_Identity=29.1322314049587, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1788811, Length=466, Percent_Identity=45.2789699570815, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1789606, Length=473, Percent_Identity=44.6088794926004, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI87082180, Length=465, Percent_Identity=41.7204301075269, Blast_Score=350, Evalue=9e-98,
Organism=Escherichia coli, GI1788468, Length=439, Percent_Identity=30.2961275626424, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1789243, Length=427, Percent_Identity=33.4894613583138, Blast_Score=164, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17570289, Length=508, Percent_Identity=35.2362204724409, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI71984108, Length=478, Percent_Identity=28.8702928870293, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17543860, Length=240, Percent_Identity=26.25, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=477, Percent_Identity=35.6394129979036, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI28574881, Length=486, Percent_Identity=38.477366255144, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24665539, Length=486, Percent_Identity=38.477366255144, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24665547, Length=486, Percent_Identity=38.477366255144, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24665543, Length=486, Percent_Identity=38.477366255144, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24640763, Length=448, Percent_Identity=29.4642857142857, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI18858217, Length=448, Percent_Identity=29.4642857142857, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 50338; Mature: 50207

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKMLQFTHLHQRQPEKRGAAERRKDFGEISTAFGASQAGEQASRCEQCGIPFCSIHCP
CCHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECC
LGNNVPDWLMLVAQDRMEEAYAVSSATNTFPEICGRICPQDRLCEGNCVLEQSTHGNVTI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEE
GAVEKHITETAFAEGWVKPVLPGAETGRSVGIVGGGPAGLAAAESLRRKGHAVTVYDRHD
EHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCC
RMGGLLIYGIPGFKLEKDVVERRIRLLAEAGVKFETGVEIGKTVSFAELRARHDAVLIAT
CCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHCCCEEEEEE
GVYKAKPLGINGADLPGVHAALDYLIAQNRRDLGETVAELDAKGRNVVVIGGGDTAMDCV
CCEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
RTAIRQGAKSVKCLYRRDRANMPGSKREVAHAEEEGVEFVWMAAPEAVTGAKKAEGVRAV
HHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCHHHCCCHHHCCHHHH
RMHLGMPDSSGRQSPVAIEGSGFTLASDMVIAALGFDPEDMPVLFGAPELKVSKWGTVET
HHHCCCCCCCCCCCCEEEECCCCEEHHCCEEEEECCCCCCCCEEECCCCEEECCCCCCCC
DATLMTSLPGVFGAGDIVRGASLVVWAIKDGRDAAEAIHRYVSAKSAQAAE
CHHHHHHCCCCCCCCHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PRKMLQFTHLHQRQPEKRGAAERRKDFGEISTAFGASQAGEQASRCEQCGIPFCSIHCP
CHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECC
LGNNVPDWLMLVAQDRMEEAYAVSSATNTFPEICGRICPQDRLCEGNCVLEQSTHGNVTI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEE
GAVEKHITETAFAEGWVKPVLPGAETGRSVGIVGGGPAGLAAAESLRRKGHAVTVYDRHD
EHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCC
RMGGLLIYGIPGFKLEKDVVERRIRLLAEAGVKFETGVEIGKTVSFAELRARHDAVLIAT
CCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHCCCEEEEEE
GVYKAKPLGINGADLPGVHAALDYLIAQNRRDLGETVAELDAKGRNVVVIGGGDTAMDCV
CCEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHH
RTAIRQGAKSVKCLYRRDRANMPGSKREVAHAEEEGVEFVWMAAPEAVTGAKKAEGVRAV
HHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCHHHCCCHHHCCHHHH
RMHLGMPDSSGRQSPVAIEGSGFTLASDMVIAALGFDPEDMPVLFGAPELKVSKWGTVET
HHHCCCCCCCCCCCCEEEECCCCEEHHCCEEEEECCCCCCCCEEECCCCEEECCCCCCCC
DATLMTSLPGVFGAGDIVRGASLVVWAIKDGRDAAEAIHRYVSAKSAQAAE
CHHHHHHCCCCCCCCHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8428988; 2198943 [H]