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The map label for this gene is fliF

Identifier: 83309602

GI number: 83309602

Start: 537136

End: 538860

Strand: Reverse

Name: fliF

Synonym: amb0503

Alternate gene names: 83309602

Gene position: 538860-537136 (Counterclockwise)

Preceding gene: 83309603

Following gene: 83309601

Centisome position: 10.85

GC content: 65.62

Gene sequence:

>1725_bases
ATGTGTGTGCCGGCCGGCGGCACCCCCCGCGCGCCTGGGCGATGGGCACCCGATGGAGACGACGTGAACGCATTCCTGCA
GATGCTGCGCGGCCTGGGGCCCATGCGGCTGGCCGCCATCGCCGGTGTCGGCGTCAGTATTCTCGGCTTCTTCATCTATC
TGATGAGCCGCGTCGCCGCGCCGCAGATGGAATTGCTGTATGGCGATCTCGATCTGGGCGATTCCAAGCAGATCATCGAG
AAGCTGACCGCCGACAAGATTCCCTACGAACTCAGGAAGGACGGCACCGAGGTCTGGGTGCCCAAAGACCGCAAGCTGGA
CCTGCGCGTGCGCATGGCCGAGCAGGCCCTGCCCGCCGGCGGCCGCATGGCCGGCTATGAATTGTTCGACAAGCAGGACG
CGCTGGGCTCCACCAGCTTCCAGCAGAACATCACCATGGTCCGCGCCCTGGAAGGCGAGCTGTCCAAGACCATCCGCTCC
ATCGACCGGGTCAAGGCGGCCCGCGTCCATCTGGTGCTGCCCAAGCGCGAGGCCTTCTCCCGCGATACCCAGGAGCCGTC
CGCCTCGGTCATCATCAAGATGAACGGCGCCCAGCGGCTGGACAAGGGCCAGGTCAGCGCCATCCAGCACCTGATCGCCG
CCGCCGTCCCCAAGATGAAGCCGTCGCGCATCTCCATCGTCGACGACAAGGGCACCCTGCTGGCCAAGGGCTTCGAGAAC
TCCAAGGAGTATCAGGCCCAGACCGCCGAGGAGATGAAGGTCAAGACCGAGGCCAAGCTGGTCAAGACCATCGAGGACCT
GCTGGAGCGTTCGCTGGGTCCGGGCCGGGTGCGCGCCGAGGTCACGGTGGACATGGACATGAGCCAGGCCACCACCACCG
AGGAAACCTTCGACCCCGAGACCAAGGTGGTCCGCTCCCAGGTCACCGTCAACGAGGGCGAGCAGAGCCAGGACGCCGAG
CCCACCCCGGTCACCGTTCAGCAGAACCTGCCCGATCCCAACGCCTCGTCCTCGGGCAACATCCGCACCTCGACCAAGAT
CAACAAGAACCAGGAAACCGTGAACTACGAGATCTCGCGCAAGACCAAGAGCACGGTCCGCGAGATGGGCGAGATCCGCA
AGGTTTCCGCCGCCGTCATCGTCGACGGCGTGCGCGAGCAGTCGGCCGACGGCAAGCAGACCTCGTACCGCGAGCGCACC
GCCGAAGAGCTGTCCCAGATCGAAGCCCTGGTCAAGTCGGCCATCGGCTACACCAAGGACCGCGATGATCAGGTCAAGGT
GGCCTCCATGCCCTTCTACAAGGGCGAGGAGCTGCTTCAGGCCGAGGAGCCGGGCGAGTTCATCTTCGGCATGCGCCGCG
ACTTCGTCGAGAAGATCGCCTCGAATCTGGGCCTCTCGGTGGTGGCCATCCTGTTCCTGCTGCTGGTGCTGCGTCCGCTC
ATCAGCCGGGCCATCGAGTCCATGCAGGGTCAGGTCGGCCCCGACGGCCGCCGCCTGCTGACCACCGATGGCGGCGCCAT
GCCGCAGCTCACCGGCCCCGGCGCCGCCGCCATGCCGGCCCCGCTGGGCGGCGAGGAAGAGGTCATCGCCGACGAACTGA
TCGACATCGACAAGGTGGAAGGCCGCGTCAAGGCCTCCTCGATCCGCAAGATCGGCGAAATCGTCGAGAAGCATCCGGAA
GAGGCTCTGTCCATCATCCGCAACTGGCTCTACCAGGAAAGCTGA

Upstream 100 bases:

>100_bases
GGCGCCTGAGGGGCTCACGCAATACAGCAAAACCCCACCCGGCAAAAATTGCCGGGCGTTAACCATCTCTTCAGCGGCAA
GACCTTAGTCTGTCACCTGG

Downstream 100 bases:

>100_bases
TAGGGAACCGCCATGGCACGCGTCAAGGAAGACTACCGCAGCCTCACCGGCCCCCAGAAGGCGGCCATGTTCATGCTGTC
GCTCAACGAAGAGCACTCGT

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MCVPAGGTPRAPGRWAPDGDDVNAFLQMLRGLGPMRLAAIAGVGVSILGFFIYLMSRVAAPQMELLYGDLDLGDSKQIIE
KLTADKIPYELRKDGTEVWVPKDRKLDLRVRMAEQALPAGGRMAGYELFDKQDALGSTSFQQNITMVRALEGELSKTIRS
IDRVKAARVHLVLPKREAFSRDTQEPSASVIIKMNGAQRLDKGQVSAIQHLIAAAVPKMKPSRISIVDDKGTLLAKGFEN
SKEYQAQTAEEMKVKTEAKLVKTIEDLLERSLGPGRVRAEVTVDMDMSQATTTEETFDPETKVVRSQVTVNEGEQSQDAE
PTPVTVQQNLPDPNASSSGNIRTSTKINKNQETVNYEISRKTKSTVREMGEIRKVSAAVIVDGVREQSADGKQTSYRERT
AEELSQIEALVKSAIGYTKDRDDQVKVASMPFYKGEELLQAEEPGEFIFGMRRDFVEKIASNLGLSVVAILFLLLVLRPL
ISRAIESMQGQVGPDGRRLLTTDGGAMPQLTGPGAAAMPAPLGGEEEVIADELIDIDKVEGRVKASSIRKIGEIVEKHPE
EALSIIRNWLYQES

Sequences:

>Translated_574_residues
MCVPAGGTPRAPGRWAPDGDDVNAFLQMLRGLGPMRLAAIAGVGVSILGFFIYLMSRVAAPQMELLYGDLDLGDSKQIIE
KLTADKIPYELRKDGTEVWVPKDRKLDLRVRMAEQALPAGGRMAGYELFDKQDALGSTSFQQNITMVRALEGELSKTIRS
IDRVKAARVHLVLPKREAFSRDTQEPSASVIIKMNGAQRLDKGQVSAIQHLIAAAVPKMKPSRISIVDDKGTLLAKGFEN
SKEYQAQTAEEMKVKTEAKLVKTIEDLLERSLGPGRVRAEVTVDMDMSQATTTEETFDPETKVVRSQVTVNEGEQSQDAE
PTPVTVQQNLPDPNASSSGNIRTSTKINKNQETVNYEISRKTKSTVREMGEIRKVSAAVIVDGVREQSADGKQTSYRERT
AEELSQIEALVKSAIGYTKDRDDQVKVASMPFYKGEELLQAEEPGEFIFGMRRDFVEKIASNLGLSVVAILFLLLVLRPL
ISRAIESMQGQVGPDGRRLLTTDGGAMPQLTGPGAAAMPAPLGGEEEVIADELIDIDKVEGRVKASSIRKIGEIVEKHPE
EALSIIRNWLYQES
>Mature_574_residues
MCVPAGGTPRAPGRWAPDGDDVNAFLQMLRGLGPMRLAAIAGVGVSILGFFIYLMSRVAAPQMELLYGDLDLGDSKQIIE
KLTADKIPYELRKDGTEVWVPKDRKLDLRVRMAEQALPAGGRMAGYELFDKQDALGSTSFQQNITMVRALEGELSKTIRS
IDRVKAARVHLVLPKREAFSRDTQEPSASVIIKMNGAQRLDKGQVSAIQHLIAAAVPKMKPSRISIVDDKGTLLAKGFEN
SKEYQAQTAEEMKVKTEAKLVKTIEDLLERSLGPGRVRAEVTVDMDMSQATTTEETFDPETKVVRSQVTVNEGEQSQDAE
PTPVTVQQNLPDPNASSSGNIRTSTKINKNQETVNYEISRKTKSTVREMGEIRKVSAAVIVDGVREQSADGKQTSYRERT
AEELSQIEALVKSAIGYTKDRDDQVKVASMPFYKGEELLQAEEPGEFIFGMRRDFVEKIASNLGLSVVAILFLLLVLRPL
ISRAIESMQGQVGPDGRRLLTTDGGAMPQLTGPGAAAMPAPLGGEEEVIADELIDIDKVEGRVKASSIRKIGEIVEKHPE
EALSIIRNWLYQES

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=450, Percent_Identity=32, Blast_Score=211, Evalue=1e-55,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 62980; Mature: 62980

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVPAGGTPRAPGRWAPDGDDVNAFLQMLRGLGPMRLAAIAGVGVSILGFFIYLMSRVAA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PQMELLYGDLDLGDSKQIIEKLTADKIPYELRKDGTEVWVPKDRKLDLRVRMAEQALPAG
CCHHHEEECCCCCCHHHHHHHHHHCCCCHHHHCCCCEEECCCCCCCCHHEEEHHHCCCCC
GRMAGYELFDKQDALGSTSFQQNITMVRALEGELSKTIRSIDRVKAARVHLVLPKREAFS
CCCCCHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHC
RDTQEPSASVIIKMNGAQRLDKGQVSAIQHLIAAAVPKMKPSRISIVDDKGTLLAKGFEN
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCC
SKEYQAQTAEEMKVKTEAKLVKTIEDLLERSLGPGRVRAEVTVDMDMSQATTTEETFDPE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHCCCH
TKVVRSQVTVNEGEQSQDAEPTPVTVQQNLPDPNASSSGNIRTSTKINKNQETVNYEISR
HHHHHHHHEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCCCCCEEEEEEHH
KTKSTVREMGEIRKVSAAVIVDGVREQSADGKQTSYRERTAEELSQIEALVKSAIGYTKD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RDDQVKVASMPFYKGEELLQAEEPGEFIFGMRRDFVEKIASNLGLSVVAILFLLLVLRPL
CCCCEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ISRAIESMQGQVGPDGRRLLTTDGGAMPQLTGPGAAAMPAPLGGEEEVIADELIDIDKVE
HHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GRVKASSIRKIGEIVEKHPEEALSIIRNWLYQES
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCVPAGGTPRAPGRWAPDGDDVNAFLQMLRGLGPMRLAAIAGVGVSILGFFIYLMSRVAA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PQMELLYGDLDLGDSKQIIEKLTADKIPYELRKDGTEVWVPKDRKLDLRVRMAEQALPAG
CCHHHEEECCCCCCHHHHHHHHHHCCCCHHHHCCCCEEECCCCCCCCHHEEEHHHCCCCC
GRMAGYELFDKQDALGSTSFQQNITMVRALEGELSKTIRSIDRVKAARVHLVLPKREAFS
CCCCCHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHC
RDTQEPSASVIIKMNGAQRLDKGQVSAIQHLIAAAVPKMKPSRISIVDDKGTLLAKGFEN
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEECCCCC
SKEYQAQTAEEMKVKTEAKLVKTIEDLLERSLGPGRVRAEVTVDMDMSQATTTEETFDPE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHCCCH
TKVVRSQVTVNEGEQSQDAEPTPVTVQQNLPDPNASSSGNIRTSTKINKNQETVNYEISR
HHHHHHHHEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCCCCCEEEEEEHH
KTKSTVREMGEIRKVSAAVIVDGVREQSADGKQTSYRERTAEELSQIEALVKSAIGYTKD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RDDQVKVASMPFYKGEELLQAEEPGEFIFGMRRDFVEKIASNLGLSVVAILFLLLVLRPL
CCCCEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ISRAIESMQGQVGPDGRRLLTTDGGAMPQLTGPGAAAMPAPLGGEEEVIADELIDIDKVE
HHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GRVKASSIRKIGEIVEKHPEEALSIIRNWLYQES
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8002583; 11259647 [H]