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The map label for this gene is flbD [H]

Identifier: 83309598

GI number: 83309598

Start: 533683

End: 535056

Strand: Reverse

Name: flbD [H]

Synonym: amb0499

Alternate gene names: 83309598

Gene position: 535056-533683 (Counterclockwise)

Preceding gene: 83309599

Following gene: 83309597

Centisome position: 10.77

GC content: 66.52

Gene sequence:

>1374_bases
ATGCGACTTCTGATCATCGGCACGCTCGACGGCCAGATCGGCGCCGCCAGCCAGATCGCCATGGCGCGGGGCGCCAAGGT
CCGTCAGGCCGAGGACGTGCCCGCCGGCCTGGAGGTGCTGCGCGCCCAGGGCGCCGATCTGGTGATGATCGACGTCAAGC
GCGACATCAAGGGGCTGATCGATTCGCTGGAAGCCGAACGCATCATCGTTCCGGTGGTGGCCTGCGGCATCGCGTCCGAC
GCCTCGGCCGCCGTGCGCGCCATCAAGGCCGGCGCCAAGGAATACATCCCGCTGCCGCCCGACGCCGAGCTGATCGCCGC
CGTCATCGCCGCCGTCACCCAGGAAAACCACAACATCATCTTCAAGGACCCCCGCATGGGCCAGACCTTGAAGCTGGCCG
AGCAGGTGGCCAATTCCGACGCCTCCATCATGATCACCGGCGAATCGGGCACCGGCAAGGAGCTGATCGCCCGGTTCATC
CACAACAAGTCCAAGCGCTCGGACAAGCGCTTCGTGGCTGTCAACTGCGCCGCCATCCCCGAGAACCTGCTGGAATCCGA
GCTGTTTGGCCATGAAAAGGGCGCCTTCACCGGCGCCGTGGCCCGGCGCATCGGCAAGTTCGAGGAGGCCCAGGGCGGCA
CCTTGCTGCTGGACGAAATCTCCGAGATGGACGTGCGCCTGCAATCCAAGCTGCTGCGCGCCATCCAGGAAAAGGAAATC
GACCGGGTGGGCGGCAACCAGCCGGTCAAGGTGGACGTGCGCATTCTCGCCACCTCCAACCGCACCATGGAGGACGAGGT
CCGCAAGGGCACCTTCCGCGAGGACCTCTATTACCGCCTCAACGTGGTGACCCTGGCGCTGCCCGCGCTGCGCGAGCGGC
CGCTGGACATCGCGGTGCTGGCCGAACACTTCGTCAAGCGCTACACCGAGGCCAACGGCCTGCCCACCCGCCAGCTTTCC
GCCGAAGCGCGGCAATTGCTGTCGCGCCACCACTGGCGCGGCAATGTGCGCGAGCTGGAAAACACCATGCACCGCGCCGT
GCTGCTGGCCAGCGGCGCCGAGATCGGCCCCGAGGCCATGATCCTGTCGGGCAACCCGGTGGGCACCAATCCCGATCCCA
TGGTCGCCCAGGCGGCCAATGCCGCTGCCTCGGTCATCGGGGCGAGGGGCGGCATGGTCGGCAAGACGGTGGCCGAGGTC
GAGCGCGACCTGATCATCGACACCTTGTCCCATTGCCTGGGCAACCGCACCCATGCGGCCAACATCTTAGGGATTTCCAT
CCGCACCCTTCGCAACAAGCTGAAGCAGTATTCCGACGAAGGCATCGACGTGCCGCCGCCCGCCGGCGGCGAAATCGAAC
GAGCCATGATCTAG

Upstream 100 bases:

>100_bases
TGGCGAAGTGGTGGTGGTCGAGGATCGCCTGGGCGTGACCATGACCGAAATCATCAAGACCGACCGGGCCTAAGGGTCCT
GACGGCGGGAGAGGGATGAC

Downstream 100 bases:

>100_bases
CATCATGGCTGAAGGACAGGCATCAGCGTCCAGCGTCGGCCGGGAAATCCTCGATCGCTTTGCCGCGTCCGCGCGGCGCG
GCGACGTCGGCCTGGCGTTC

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRLLIIGTLDGQIGAASQIAMARGAKVRQAEDVPAGLEVLRAQGADLVMIDVKRDIKGLIDSLEAERIIVPVVACGIASD
ASAAVRAIKAGAKEYIPLPPDAELIAAVIAAVTQENHNIIFKDPRMGQTLKLAEQVANSDASIMITGESGTGKELIARFI
HNKSKRSDKRFVAVNCAAIPENLLESELFGHEKGAFTGAVARRIGKFEEAQGGTLLLDEISEMDVRLQSKLLRAIQEKEI
DRVGGNQPVKVDVRILATSNRTMEDEVRKGTFREDLYYRLNVVTLALPALRERPLDIAVLAEHFVKRYTEANGLPTRQLS
AEARQLLSRHHWRGNVRELENTMHRAVLLASGAEIGPEAMILSGNPVGTNPDPMVAQAANAAASVIGARGGMVGKTVAEV
ERDLIIDTLSHCLGNRTHAANILGISIRTLRNKLKQYSDEGIDVPPPAGGEIERAMI

Sequences:

>Translated_457_residues
MRLLIIGTLDGQIGAASQIAMARGAKVRQAEDVPAGLEVLRAQGADLVMIDVKRDIKGLIDSLEAERIIVPVVACGIASD
ASAAVRAIKAGAKEYIPLPPDAELIAAVIAAVTQENHNIIFKDPRMGQTLKLAEQVANSDASIMITGESGTGKELIARFI
HNKSKRSDKRFVAVNCAAIPENLLESELFGHEKGAFTGAVARRIGKFEEAQGGTLLLDEISEMDVRLQSKLLRAIQEKEI
DRVGGNQPVKVDVRILATSNRTMEDEVRKGTFREDLYYRLNVVTLALPALRERPLDIAVLAEHFVKRYTEANGLPTRQLS
AEARQLLSRHHWRGNVRELENTMHRAVLLASGAEIGPEAMILSGNPVGTNPDPMVAQAANAAASVIGARGGMVGKTVAEV
ERDLIIDTLSHCLGNRTHAANILGISIRTLRNKLKQYSDEGIDVPPPAGGEIERAMI
>Mature_457_residues
MRLLIIGTLDGQIGAASQIAMARGAKVRQAEDVPAGLEVLRAQGADLVMIDVKRDIKGLIDSLEAERIIVPVVACGIASD
ASAAVRAIKAGAKEYIPLPPDAELIAAVIAAVTQENHNIIFKDPRMGQTLKLAEQVANSDASIMITGESGTGKELIARFI
HNKSKRSDKRFVAVNCAAIPENLLESELFGHEKGAFTGAVARRIGKFEEAQGGTLLLDEISEMDVRLQSKLLRAIQEKEI
DRVGGNQPVKVDVRILATSNRTMEDEVRKGTFREDLYYRLNVVTLALPALRERPLDIAVLAEHFVKRYTEANGLPTRQLS
AEARQLLSRHHWRGNVRELENTMHRAVLLASGAEIGPEAMILSGNPVGTNPDPMVAQAANAAASVIGARGGMVGKTVAEV
ERDLIIDTLSHCLGNRTHAANILGISIRTLRNKLKQYSDEGIDVPPPAGGEIERAMI

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=465, Percent_Identity=38.0645161290323, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1790437, Length=418, Percent_Identity=41.6267942583732, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1790299, Length=432, Percent_Identity=38.8888888888889, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1788905, Length=432, Percent_Identity=37.2685185185185, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI87082117, Length=264, Percent_Identity=48.4848484848485, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1789087, Length=241, Percent_Identity=51.4522821576763, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI87082152, Length=282, Percent_Identity=47.5177304964539, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789233, Length=315, Percent_Identity=42.2222222222222, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI1787583, Length=331, Percent_Identity=38.6706948640483, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1786524, Length=337, Percent_Identity=40.3560830860534, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI87081872, Length=325, Percent_Identity=38.4615384615385, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=36.8421052631579, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081858, Length=347, Percent_Identity=27.0893371757925, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49354; Mature: 49354

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLIIGTLDGQIGAASQIAMARGAKVRQAEDVPAGLEVLRAQGADLVMIDVKRDIKGLI
CEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEECHHHHHHHH
DSLEAERIIVPVVACGIASDASAAVRAIKAGAKEYIPLPPDAELIAAVIAAVTQENHNII
HHHCCCCEEEEHHHHCCCCCHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHCCCCCEE
FKDPRMGQTLKLAEQVANSDASIMITGESGTGKELIARFIHNKSKRSDKRFVAVNCAAIP
EECCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEEHHCC
ENLLESELFGHEKGAFTGAVARRIGKFEEAQGGTLLLDEISEMDVRLQSKLLRAIQEKEI
HHHHHHHHHCCCCCCHHHHHHHHHCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
DRVGGNQPVKVDVRILATSNRTMEDEVRKGTFREDLYYRLNVVTLALPALRERPLDIAVL
HHCCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AEHFVKRYTEANGLPTRQLSAEARQLLSRHHWRGNVRELENTMHRAVLLASGAEIGPEAM
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEE
ILSGNPVGTNPDPMVAQAANAAASVIGARGGMVGKTVAEVERDLIIDTLSHCLGNRTHAA
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
NILGISIRTLRNKLKQYSDEGIDVPPPAGGEIERAMI
CEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure
MRLLIIGTLDGQIGAASQIAMARGAKVRQAEDVPAGLEVLRAQGADLVMIDVKRDIKGLI
CEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEECHHHHHHHH
DSLEAERIIVPVVACGIASDASAAVRAIKAGAKEYIPLPPDAELIAAVIAAVTQENHNII
HHHCCCCEEEEHHHHCCCCCHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHCCCCCEE
FKDPRMGQTLKLAEQVANSDASIMITGESGTGKELIARFIHNKSKRSDKRFVAVNCAAIP
EECCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEEHHCC
ENLLESELFGHEKGAFTGAVARRIGKFEEAQGGTLLLDEISEMDVRLQSKLLRAIQEKEI
HHHHHHHHHCCCCCCHHHHHHHHHCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
DRVGGNQPVKVDVRILATSNRTMEDEVRKGTFREDLYYRLNVVTLALPALRERPLDIAVL
HHCCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AEHFVKRYTEANGLPTRQLSAEARQLLSRHHWRGNVRELENTMHRAVLLASGAEIGPEAM
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEE
ILSGNPVGTNPDPMVAQAANAAASVIGARGGMVGKTVAEVERDLIIDTLSHCLGNRTHAA
EEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
NILGISIRTLRNKLKQYSDEGIDVPPPAGGEIERAMI
CEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]