Click here to switch to the map view.

The map label for this gene is radA [H]

Identifier: 162319789

GI number: 162319789

Start: 2267224

End: 2268606

Strand: Reverse

Name: radA [H]

Synonym: amb2091

Alternate gene names: 162319789

Gene position: 2268606-2267224 (Counterclockwise)

Preceding gene: 83311191

Following gene: 83311189

Centisome position: 45.67

GC content: 69.27

Gene sequence:

>1383_bases
TTGGCCAAGTCCCCATCCTCCCAGTTCGTCTGCCAGGAATGCGGTGCCGTCACCCGCAAATGGGCGGGCAAGTGCGAAGC
CTGCGGCGCCTGGAACTCCATCACCGAGGAGGCGGCGCGCGAAGAGGTGCCCAAGGGCCTGACGGCGGGCAAGGGCAAGC
GCATCGAATTCGTCGGCCTCGAAGGCTCGGCGGCACCGCTGGCCCGCTGGCTGACCGGCATCGGCGAACTGGACCGCGTG
TGCGGCGGCGGGCTGGTGCCCGGCTCGTGCCTGCTGGTGGGCGGCGATCCCGGCATCGGTAAGTCCACTCTCTTGCTCCA
GGCCACGGCCCGGCTGTCGTCCCAGATCTCGGTCGCCTATATCTCGGGCGAGGAGGCGGTGGATCAGGTGCGCATGCGCG
CCGCCCGGTTAGGGTATGCCAAGGCCACCGTCGCCCTGGCCTCGGCCGGGTCGTTGCGCGACATCATCGCGTCGCTGGAC
CAGCCCGACGCCCCCCAGGTGGTGGTGATCGATTCCATTCAGACCGTCTATGCCGACAACATCGAATCGGCCCCCGGCAC
CGTCTCCCAGGTGCGCGCCTGCGCCGCCGAGTTGATCCGGCTGGCCAAGCGGCGGGGCTTCGTGCTGTTCCTGGTCGGCC
ACGTCACCAAGGAAGGCACCATCGCCGGCCCGCGCGTGCTGGAGCACATGGTCGACACCGTGCTGTATTTCGAGGGCGAC
CGGGGCCATCAGTTCCGCATCCTGCGCGCCGTCAAGAACCGCTTCGGCGCCACCGACGAGATCGGGGTGTTCGAGATGAC
CGAGGGCGGGCTGTCGGAAGTGGCCAACCCATCCGCCCTGTTCCTGGCCGAGCGGCGGGGCAACGTCTCGGGCTCTTGCG
TCTTCGCCGGCATGGAAGGGACACGCCCCATGCTGGTGGAAATCCAGGCCCTTGTGGCGCCAAGTTCACTGTCTTCGCCG
CGCCGGGCCGTGGTCGGCTGGGATACCAACCGCCTGTCCATGGTGCTGGCGGTGCTTGATGCCCGCTGTGGGCTTGCTTT
GTCGGGCAATGACGTTTATTTGAACGTCGCCGGAGGATTGCGGATCGCCGAGCCGGCCGCCGACCTTGCCGTCGCCGCGG
CCCTGGTGTCGTCCGCGTCCGACGTGCCCGTTCCCGCCGACATGGTCGTGTTCGGCGAGATCGGGCTGTCCGGCGAGGTC
CGGGCGGTGGCCCAGGCCGATACCCGCCTGAAGGAAGCCGCCAAGCTCGGCTTCGCCCAGGCGCTGGTGCCGGCCCGGCC
GCGCAAGGACAAGGGCGGAGGGGCTGCGGGCGCGGGGTCGCCCCTGGCTATCCGCTCCATAGGCCATTTGCAGGACGTGC
TGGGGCTGTTCCGGCACGAGTGA

Upstream 100 bases:

>100_bases
CGCCAACCGCAAATAGGCGGAAGGATCAGCATCCCTCTGGTTGCCGCAATGTTCTCGTGATAGACTGCGCCTCCAACCGC
CTTGTCGAGTAGCCCGCCCC

Downstream 100 bases:

>100_bases
CCACCCTAACTGGAAGAGGCCATGGGGAACCTCAACGTCACCGCCGTCGACGTTATTGTCGCCGTGGTGTTGCTCGGCTC
GGCGGGCTTCGCCTTCCTGC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MAKSPSSQFVCQECGAVTRKWAGKCEACGAWNSITEEAAREEVPKGLTAGKGKRIEFVGLEGSAAPLARWLTGIGELDRV
CGGGLVPGSCLLVGGDPGIGKSTLLLQATARLSSQISVAYISGEEAVDQVRMRAARLGYAKATVALASAGSLRDIIASLD
QPDAPQVVVIDSIQTVYADNIESAPGTVSQVRACAAELIRLAKRRGFVLFLVGHVTKEGTIAGPRVLEHMVDTVLYFEGD
RGHQFRILRAVKNRFGATDEIGVFEMTEGGLSEVANPSALFLAERRGNVSGSCVFAGMEGTRPMLVEIQALVAPSSLSSP
RRAVVGWDTNRLSMVLAVLDARCGLALSGNDVYLNVAGGLRIAEPAADLAVAAALVSSASDVPVPADMVVFGEIGLSGEV
RAVAQADTRLKEAAKLGFAQALVPARPRKDKGGGAAGAGSPLAIRSIGHLQDVLGLFRHE

Sequences:

>Translated_460_residues
MAKSPSSQFVCQECGAVTRKWAGKCEACGAWNSITEEAAREEVPKGLTAGKGKRIEFVGLEGSAAPLARWLTGIGELDRV
CGGGLVPGSCLLVGGDPGIGKSTLLLQATARLSSQISVAYISGEEAVDQVRMRAARLGYAKATVALASAGSLRDIIASLD
QPDAPQVVVIDSIQTVYADNIESAPGTVSQVRACAAELIRLAKRRGFVLFLVGHVTKEGTIAGPRVLEHMVDTVLYFEGD
RGHQFRILRAVKNRFGATDEIGVFEMTEGGLSEVANPSALFLAERRGNVSGSCVFAGMEGTRPMLVEIQALVAPSSLSSP
RRAVVGWDTNRLSMVLAVLDARCGLALSGNDVYLNVAGGLRIAEPAADLAVAAALVSSASDVPVPADMVVFGEIGLSGEV
RAVAQADTRLKEAAKLGFAQALVPARPRKDKGGGAAGAGSPLAIRSIGHLQDVLGLFRHE
>Mature_459_residues
AKSPSSQFVCQECGAVTRKWAGKCEACGAWNSITEEAAREEVPKGLTAGKGKRIEFVGLEGSAAPLARWLTGIGELDRVC
GGGLVPGSCLLVGGDPGIGKSTLLLQATARLSSQISVAYISGEEAVDQVRMRAARLGYAKATVALASAGSLRDIIASLDQ
PDAPQVVVIDSIQTVYADNIESAPGTVSQVRACAAELIRLAKRRGFVLFLVGHVTKEGTIAGPRVLEHMVDTVLYFEGDR
GHQFRILRAVKNRFGATDEIGVFEMTEGGLSEVANPSALFLAERRGNVSGSCVFAGMEGTRPMLVEIQALVAPSSLSSPR
RAVVGWDTNRLSMVLAVLDARCGLALSGNDVYLNVAGGLRIAEPAADLAVAAALVSSASDVPVPADMVVFGEIGLSGEVR
AVAQADTRLKEAAKLGFAQALVPARPRKDKGGGAAGAGSPLAIRSIGHLQDVLGLFRHE

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=470, Percent_Identity=47.0212765957447, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR020588
- InterPro:   IPR004504
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC [H]

EC number: NA

Molecular weight: Translated: 47910; Mature: 47778

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSPSSQFVCQECGAVTRKWAGKCEACGAWNSITEEAAREEVPKGLTAGKGKRIEFVGL
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEC
EGSAAPLARWLTGIGELDRVCGGGLVPGSCLLVGGDPGIGKSTLLLQATARLSSQISVAY
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEHHHHHHCCCEEEEE
ISGEEAVDQVRMRAARLGYAKATVALASAGSLRDIIASLDQPDAPQVVVIDSIQTVYADN
ECCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEECCHHHHHHCC
IESAPGTVSQVRACAAELIRLAKRRGFVLFLVGHVTKEGTIAGPRVLEHMVDTVLYFEGD
CCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHHHHHHEEEECC
RGHQFRILRAVKNRFGATDEIGVFEMTEGGLSEVANPSALFLAERRGNVSGSCVFAGMEG
CCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHCCCCEEEEEECCCCCCCEEEEEECCC
TRPMLVEIQALVAPSSLSSPRRAVVGWDTNRLSMVLAVLDARCGLALSGNDVYLNVAGGL
CCCEEEEEEEHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEECCCEEEEEECCCC
RIAEPAADLAVAAALVSSASDVPVPADMVVFGEIGLSGEVRAVAQADTRLKEAAKLGFAQ
EECCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ALVPARPRKDKGGGAAGAGSPLAIRSIGHLQDVLGLFRHE
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKSPSSQFVCQECGAVTRKWAGKCEACGAWNSITEEAAREEVPKGLTAGKGKRIEFVGL
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEC
EGSAAPLARWLTGIGELDRVCGGGLVPGSCLLVGGDPGIGKSTLLLQATARLSSQISVAY
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEHHHHHHCCCEEEEE
ISGEEAVDQVRMRAARLGYAKATVALASAGSLRDIIASLDQPDAPQVVVIDSIQTVYADN
ECCHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEECCHHHHHHCC
IESAPGTVSQVRACAAELIRLAKRRGFVLFLVGHVTKEGTIAGPRVLEHMVDTVLYFEGD
CCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHHHHHHEEEECC
RGHQFRILRAVKNRFGATDEIGVFEMTEGGLSEVANPSALFLAERRGNVSGSCVFAGMEG
CCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHCCCCEEEEEECCCCCCCEEEEEECCC
TRPMLVEIQALVAPSSLSSPRRAVVGWDTNRLSMVLAVLDARCGLALSGNDVYLNVAGGL
CCCEEEEEEEHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEECCCEEEEEECCCC
RIAEPAADLAVAAALVSSASDVPVPADMVVFGEIGLSGEVRAVAQADTRLKEAAKLGFAQ
EECCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
ALVPARPRKDKGGGAAGAGSPLAIRSIGHLQDVLGLFRHE
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA