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The map label for this gene is hisH

Identifier: 82752254

GI number: 82752254

Start: 2706721

End: 2707299

Strand: Reverse

Name: hisH

Synonym: SAB2551c

Alternate gene names: 82752254

Gene position: 2707299-2706721 (Counterclockwise)

Preceding gene: 82752255

Following gene: 82752253

Centisome position: 98.72

GC content: 36.61

Gene sequence:

>579_bases
ATGATTGTCATCGTTGATTATGGATTAGGGAATATTAGTAATGTAAAACGCGCTATTGAACATTTGGGGTATGAGGTGGT
TGTCTCAAATAAGCAAAATATAATCGATCAAGCAGAAACAATCATATTACCGGGTGTCGGTCATTTTAAAGATGCGATGT
CAGAGATAAAGCGATTAAATCTCGATGCAATATTGGCTAAGAATACTGATAAGAAAATGATCGGTATTTGCTTAGGCATG
CAATTAATGTATGAGCATAGTGATGAAGGCGATGCATCTGGATTAGGGTTTATCCCAGGAAATATTTCGCGTATCCAAAC
AGAATACCCAGTGCCGCACTTAGGTTGGAATAATTTAGTGAGTAAGCATCCTATGTTAAATCAAGATGTTTACTTCGTAC
ATTCTTACCAAGCGCCGATGTCAGAAAATGTAATTGCATATGCGCAGTATGGTACTGATATTCCAGCAATCGTCCAATTT
AACAATTATATTGGTATTCAATTCCATCCTGAAAAAAGCGGTACATATGGGTTACAAATTTTGCGTCAGGCAATACAAGG
GGGATTTATAAATGATTGA

Upstream 100 bases:

>100_bases
ATGAAATTGAAGCTATATTCAAAGCATTTTCCCGTGCATTAGGCATAGCGCTAACTGCAACTGATGATCAGCGTGTGCCG
TCATCGAAAGGTGTGATTGA

Downstream 100 bases:

>100_bases
ATTATGGCCAGCGATTGATTTGATTGGGTCAACAAGTGTGAGGTTAACAGAGGGTAAATATGATAGTGAAGAAAAAATGT
CGCGTTCGGCTGAAGAAAGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNKQNIIDQAETIILPGVGHFKDAMSEIKRLNLDAILAKNTDKKMIGICLGM
QLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGTDIPAIVQF
NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND

Sequences:

>Translated_192_residues
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNKQNIIDQAETIILPGVGHFKDAMSEIKRLNLDAILAKNTDKKMIGICLGM
QLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGTDIPAIVQF
NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND
>Mature_192_residues
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNKQNIIDQAETIILPGVGHFKDAMSEIKRLNLDAILAKNTDKKMIGICLGM
QLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGTDIPAIVQF
NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=195, Percent_Identity=36.4102564102564, Blast_Score=124, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=33.1730769230769, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_STAAB (Q2YZ69)

Other databases:

- EMBL:   AJ938182
- RefSeq:   YP_417995.1
- ProteinModelPortal:   Q2YZ69
- SMR:   Q2YZ69
- STRING:   Q2YZ69
- EnsemblBacteria:   EBSTAT00000011139
- GeneID:   3794918
- GenomeReviews:   AJ938182_GR
- KEGG:   sab:SAB2551c
- eggNOG:   COG0118
- GeneTree:   EBGT00050000024694
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- PhylomeDB:   Q2YZ69
- ProtClustDB:   PRK13142
- BioCyc:   SAUR273036:SAB2551C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 21365; Mature: 21365

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 77-77 ACT_SITE 169-169 ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNKQNIIDQAETIILPGVGHFKDAMSEIKRLN
CEEEEECCCCCHHHHHHHHHHCCEEEEECCCHHHHHCCCEEEECCCCHHHHHHHHHHHCC
LDAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLV
HHEEEECCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHEEEECCCCCCCCHHHHH
SKHPMLNQDVYFVHSYQAPMSENVIAYAQYGTDIPAIVQFNNYIGIQFHPEKSGTYGLQI
HCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEEECCEEEEEEECCCCCCHHHHH
LRQAIQGGFIND
HHHHHCCCCCCC
>Mature Secondary Structure
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNKQNIIDQAETIILPGVGHFKDAMSEIKRLN
CEEEEECCCCCHHHHHHHHHHCCEEEEECCCHHHHHCCCEEEECCCCHHHHHHHHHHHCC
LDAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLV
HHEEEECCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHEEEECCCCCCCCHHHHH
SKHPMLNQDVYFVHSYQAPMSENVIAYAQYGTDIPAIVQFNNYIGIQFHPEKSGTYGLQI
HCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEEECCEEEEEEECCCCCCHHHHH
LRQAIQGGFIND
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA