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The map label for this gene is adaB

Identifier: 82752130

GI number: 82752130

Start: 2557761

End: 2558282

Strand: Reverse

Name: adaB

Synonym: SAB2422c

Alternate gene names: 82752130

Gene position: 2558282-2557761 (Counterclockwise)

Preceding gene: 82752132

Following gene: 82752129

Centisome position: 93.28

GC content: 38.89

Gene sequence:

>522_bases
ATGGTATATAAGAGTTACTATGATTCGCCTGTAGGACGATTGGAACTTGTGAGTGATGGGGTAAGTCTGATTGCTGTTTT
GTTTGAAAATCAACAAGATGATAGTACTAGGGAAGAAAATACGTCTTTAGCGATATTTAAAGAGGCGACACAGTGGTTAG
ACGCGTATTTTAAAGGTGACAATCCTGAAATCACAATACCTTTAAAGCCAACAGGTAGTCATTTTCAACAGTGTGTTTGG
AATGAATTAAGACAGATTCCTTATGGGACGTTAACAACGTATGGTGCTATTGCCAAAAAAGTAGGCAAGGTGCTTGATAA
GGCAAAAATGTCAGCCCAAGCGGTTGGAGGTGCAGTCGGAAGTAATCCATTATCTATTATCGTGCCATGTCATCGTGTTG
TCGGTAAAACGGGTAGCTTAACAGGATTTGGTGGAACGATTAATAATAAAATTAAGCTGTTAGAGTTGGAGAATATCGAT
ATGTCAAAGCTTTATGTACCAAAACATAGCACGAAGCCGTAA

Upstream 100 bases:

>100_bases
TGATTTGGAAGTGAATAGTTGATCTTGATAAAGCATAAAGTGTTAATCATTTTCATAAAGTTAAATGAATAGTATGATGA
TGCAAAAGGAGTGTGTGTTT

Downstream 100 bases:

>100_bases
CGTATAAATATGTTAGAAAAATAAATCTATGCATTGTTAAATACGTTAAATTATTTTAAAAACTAATCAAACTAAATTAA
TGATAATTTTAGAAAATTAA

Product: methylated-DNA-protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MVYKSYYDSPVGRLELVSDGVSLIAVLFENQQDDSTREENTSLAIFKEATQWLDAYFKGDNPEITIPLKPTGSHFQQCVW
NELRQIPYGTLTTYGAIAKKVGKVLDKAKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
MSKLYVPKHSTKP

Sequences:

>Translated_173_residues
MVYKSYYDSPVGRLELVSDGVSLIAVLFENQQDDSTREENTSLAIFKEATQWLDAYFKGDNPEITIPLKPTGSHFQQCVW
NELRQIPYGTLTTYGAIAKKVGKVLDKAKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
MSKLYVPKHSTKP
>Mature_173_residues
MVYKSYYDSPVGRLELVSDGVSLIAVLFENQQDDSTREENTSLAIFKEATQWLDAYFKGDNPEITIPLKPTGSHFQQCVW
NELRQIPYGTLTTYGAIAKKVGKVLDKAKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
MSKLYVPKHSTKP

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=175, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1787596, Length=106, Percent_Identity=44.3396226415094, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1788542, Length=113, Percent_Identity=35.3982300884956, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=107, Percent_Identity=42.9906542056075, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18949; Mature: 18949

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYKSYYDSPVGRLELVSDGVSLIAVLFENQQDDSTREENTSLAIFKEATQWLDAYFKGD
CCCCCCCCCCCCCEEEHHCCHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCC
NPEITIPLKPTGSHFQQCVWNELRQIPYGTLTTYGAIAKKVGKVLDKAKMSAQAVGGAVG
CCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENIDMSKLYVPKHSTKP
CCCEEEEEECHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCC
>Mature Secondary Structure
MVYKSYYDSPVGRLELVSDGVSLIAVLFENQQDDSTREENTSLAIFKEATQWLDAYFKGD
CCCCCCCCCCCCCEEEHHCCHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCC
NPEITIPLKPTGSHFQQCVWNELRQIPYGTLTTYGAIAKKVGKVLDKAKMSAQAVGGAVG
CCEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENIDMSKLYVPKHSTKP
CCCEEEEEECHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]