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The map label for this gene is gltS [H]

Identifier: 82751940

GI number: 82751940

Start: 2352982

End: 2354190

Strand: Direct

Name: gltS [H]

Synonym: SAB2224

Alternate gene names: 82751940

Gene position: 2352982-2354190 (Clockwise)

Preceding gene: 82751939

Following gene: 82751943

Centisome position: 85.8

GC content: 32.84

Gene sequence:

>1209_bases
ATGATAGAACTTAATGCAATTACAACATTATGTTTAGCCTGTATACTTTACTTACTTGGTAAAGCTATCGTTAATCATGT
TAATTTTTTAAAACGCATTTGTATACCAGCACCAGTGATTGGCGGCTTAATCTTTGCTATTTTAGTTGCGGCTTTGGATT
CATTTGGCATGGTTAAGATTAAATTAGATGCTTCATTCATTCAAGATTTCTTCATGTTAGCATTCTTTACGACAATCGGT
CTTGGTGCATCATTGAAATTATTTAAATTAGGTGGCAAAGTCTTGCTATTATACTTTATGTTTTGTGCTATCATTTCAGT
CATTCAAAACATAGTTGGTGTATCACTAGCAAAAGTATTAAATATTAAACCGTTACTAGGATTAACAGCTGGTTCTATGT
CTATGGAAGGTGGTCACGGTAATGCTGCCGCTTATGGTAAGACAATCCAAGATTTAGGCATTGACTCAGCATTGACAGCA
GCACTTGCGGCTGCAACTTTAGGTCTTGTATTTGGAGGGCTTATCGGTGGACCAGTTGTAAAATTTTTAATCAAACGTTA
TAACTTAAAGCCTCAACATAGTGACGTTACATTTAAAGATTATAGCCAAGTAGCATATAACGAACATTTACATAGTAAAT
TTAATGCCACTGAAGTATTCTTCATTCAATTTACAATCGTTGTATTCTGTATGGCAGTTGGAAGTTATTTCAGTCATTTA
TTTACAGCTCAAACAGGGATTAATGTTCCAATTTACGTTGGCTCATTATTTGTAGCTGTTATTGTCCGAAATATCTCTGA
AAGTTTTAATTTTAATATTGTAGATTTAAAAATTACTAATCAAATTGGCGATGTCGCATTAGGTATTTTCTTATCTCTTG
CGCTAATGAGCATTCAATTAATCGAAATTTATAAACTTGCTATACCTCTTATTATTATCGTTTTAGTTCAAGTTGTCGTT
ATGATTTTATTTGCTGTTTTAATTTTATTTAGAGGTTTAGGAAAAGATTATGATGCTGCAGTAATGGTAGGTGGTTTTAT
CGGGCATGGGCTTGGTGCAACGCCAAATGCCATGGCAAATTTAGATGTTATTACTAAAAAATATGGAAACTCACCTAAAG
CATATTTAGTTGTGCCTATTGTTGGTGCATTCTTAATCGATTTAATTGGTGTTATAGTCATTATGGGATTCATTCAATGG
TTTAGTTAA

Upstream 100 bases:

>100_bases
ATTGATTTGTTGCATGTATAATGTTAAAAGCGCCCTTTTATAACGCTTACATATAAAAGCTTATTTAGGGAGAGGGATAT
TCAACAAGGGGGATTTGAAA

Downstream 100 bases:

>100_bases
ACACCAAACTCATAAATAAAAGAGGAGGCCTTCGCTTCCTCTTTTATTTATCCTCGATGTATATTCAAGTTACGTTGTTC
TATCCATGACAATATTTCCG

Product: sodium/glutamate symporter

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLIFAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIG
LGASLKLFKLGGKVLLLYFMFCAIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDVTFKDYSQVAYNEHLHSKFNATEVFFIQFTIVVFCMAVGSYFSHL
FTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDLKITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVV
MILFAVLILFRGLGKDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVIVIMGFIQW
FS

Sequences:

>Translated_402_residues
MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLIFAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIG
LGASLKLFKLGGKVLLLYFMFCAIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDVTFKDYSQVAYNEHLHSKFNATEVFFIQFTIVVFCMAVGSYFSHL
FTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDLKITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVV
MILFAVLILFRGLGKDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVIVIMGFIQW
FS
>Mature_402_residues
MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLIFAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIG
LGASLKLFKLGGKVLLLYFMFCAIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDVTFKDYSQVAYNEHLHSKFNATEVFFIQFTIVVFCMAVGSYFSHL
FTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDLKITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVV
MILFAVLILFRGLGKDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVIVIMGFIQW
FS

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=379, Percent_Identity=38.5224274406332, Blast_Score=220, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 43249; Mature: 43249

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLIFAILVAALDSFGMVKI
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEE
KLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFCAIISVIQNIVGVSLAKVL
EECHHHHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTAALAAATLGLVFGGLIGGPVV
CCCHHHCCCCCCCCCCCCCCCHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KFLIKRYNLKPQHSDVTFKDYSQVAYNEHLHSKFNATEVFFIQFTIVVFCMAVGSYFSHL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDLKITNQIGDVALGIFLSLALMSIQL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHH
IEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLGKDYDAAVMVGGFIGHGLGATPNAMAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHH
LDVITKKYGNSPKAYLVVPIVGAFLIDLIGVIVIMGFIQWFS
HHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]