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The map label for this gene is truA

Identifier: 82751809

GI number: 82751809

Start: 2240887

End: 2241690

Strand: Reverse

Name: truA

Synonym: SAB2092c

Alternate gene names: 82751809

Gene position: 2241690-2240887 (Counterclockwise)

Preceding gene: 82751810

Following gene: 82751808

Centisome position: 81.74

GC content: 34.2

Gene sequence:

>804_bases
ATGCGTATATTAGTAGAAATTGCGTATCAAGGAAATAATTTTCTAGGTTTTCAAATTCAACAAAATGGTCGAACGGTACA
GCAACAATTTGAAAAGCTATTACAACGCATGCATAAAAGACATGTGAGAATCCATCCTTCAAGTAGGACTGATAGAGGTG
TACATGCGATACAGCAATACTTTCATTTCGACACAGAGCTGAATATACCTATGTCACAATGGCAATATGCAATGAACCGA
ACGTTGCCTGTTGATATTTATGTCAACAATGTAGTTACAGTCGATGACGATTTTCATTGTCGTTACGATTGTGTCGGTAA
ACGCTATCGATATAAAGTATATCAAGCACAACACCGCGATCCATTTCAAAGAGGTTTGAAAACATTTATTCCTGAACCGT
TGGATTTAGATAAAATGAACAGAGCGGCACAACAATTCATAGGTACACATGATTTTACGGGATTTTGTTCGCAAAAAACT
GAAGTAGAAAGTAAGGTTAGAACATTATACCAAAGTGAAATTGTAAAGACAGATGATGGCTTTGATTATATTGTCACTGG
ATCTGGATTTTTATATAACATGGTGCGTGTCTTAGTTGCATTTTTAATAGAAGTTGGAAAAGGGCGACATGAAGTATCTG
ATGTACCAAAACTTCTTGAATCAAAGAATCGTAAGAATGTACCTTTTACTGCACCGGCTGAAGGATTATATTTAGAGAAA
ATTTATTTAGATGAAAATGAGTTATTAAAAGACTTTGGTAACGATATCAAAATACATCGTAAAAAATCATTACAAAATGA
CTGA

Upstream 100 bases:

>100_bases
AGACAGCTTAAATGGCAATTGAGAGATACGTTATCTTTAATCATGATTATCCCAATTGCAATTATTTTATTCGTATTAAA
ATATTCAGGAGTGTAAATTC

Downstream 100 bases:

>100_bases
TTGCCATTGACAAAAAGCTTGTGAAATTATAAGATTATTAACGGTATTGTTTTATATCCACCCCACGATAAGCCCCGGAA
ACTTATTGTGTTACAAGATA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRIHPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNR
TLPVDIYVNNVVTVDDDFHCRYDCVGKRYRYKVYQAQHRDPFQRGLKTFIPEPLDLDKMNRAAQQFIGTHDFTGFCSQKT
EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKLLESKNRKNVPFTAPAEGLYLEK
IYLDENELLKDFGNDIKIHRKKSLQND

Sequences:

>Translated_267_residues
MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRIHPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNR
TLPVDIYVNNVVTVDDDFHCRYDCVGKRYRYKVYQAQHRDPFQRGLKTFIPEPLDLDKMNRAAQQFIGTHDFTGFCSQKT
EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKLLESKNRKNVPFTAPAEGLYLEK
IYLDENELLKDFGNDIKIHRKKSLQND
>Mature_267_residues
MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRIHPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNR
TLPVDIYVNNVVTVDDDFHCRYDCVGKRYRYKVYQAQHRDPFQRGLKTFIPEPLDLDKMNRAAQQFIGTHDFTGFCSQKT
EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKLLESKNRKNVPFTAPAEGLYLEK
IYLDENELLKDFGNDIKIHRKKSLQND

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=271, Percent_Identity=32.1033210332103, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI199559785, Length=267, Percent_Identity=26.2172284644195, Blast_Score=85, Evalue=6e-17,
Organism=Escherichia coli, GI1788657, Length=251, Percent_Identity=34.6613545816733, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71985046, Length=163, Percent_Identity=30.0613496932515, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17565126, Length=296, Percent_Identity=24.6621621621622, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321112, Length=233, Percent_Identity=24.8927038626609, Blast_Score=75, Evalue=9e-15,
Organism=Drosophila melanogaster, GI116007752, Length=309, Percent_Identity=28.8025889967638, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 31387; Mature: 31387

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRIHPSSRTDRGVHAIQQY
CEEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHH
FHFDTELNIPMSQWQYAMNRTLPVDIYVNNVVTVDDDFHCRYDCVGKRYRYKVYQAQHRD
HHCCCCCCCCHHHHHHHHCCCCEEEEEECCEEEECCCCCEEHHHHCCHHHHHHHHHHCCC
PFQRGLKTFIPEPLDLDKMNRAAQQFIGTHDFTGFCSQKTEVESKVRTLYQSEIVKTDDG
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHEECCCC
FDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKLLESKNRKNVPFTAPAEGLYLEK
CEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
IYLDENELLKDFGNDIKIHRKKSLQND
EEECHHHHHHHCCCCEEEEEHHCCCCC
>Mature Secondary Structure
MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRIHPSSRTDRGVHAIQQY
CEEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHH
FHFDTELNIPMSQWQYAMNRTLPVDIYVNNVVTVDDDFHCRYDCVGKRYRYKVYQAQHRD
HHCCCCCCCCHHHHHHHHCCCCEEEEEECCEEEECCCCCEEHHHHCCHHHHHHHHHHCCC
PFQRGLKTFIPEPLDLDKMNRAAQQFIGTHDFTGFCSQKTEVESKVRTLYQSEIVKTDDG
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHEECCCC
FDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKLLESKNRKNVPFTAPAEGLYLEK
CEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
IYLDENELLKDFGNDIKIHRKKSLQND
EEECHHHHHHHCCCCEEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA