Definition Staphylococcus aureus RF122, complete genome.
Accession NC_007622
Length 2,742,531

Click here to switch to the map view.

The map label for this gene is yqhE [C]

Identifier: 82751797

GI number: 82751797

Start: 2228539

End: 2229387

Strand: Reverse

Name: yqhE [C]

Synonym: SAB2080c

Alternate gene names: 82751797

Gene position: 2229387-2228539 (Counterclockwise)

Preceding gene: 82751798

Following gene: 82751796

Centisome position: 81.29

GC content: 33.1

Gene sequence:

>849_bases
ATGAATCATATTGAAATAAGTAAAGATGTAAAGATACCAGTATTAGGTTTCGGAGTATTTCAAATTCCTCAAGAACAAAC
AGCAGAAGCGGTTAAAGAAGCAATTAAAGCGGGTTATAGACATATTGATACAGCACAAAGTTATTTAAATGAAACTGAAG
TAGGTCAAGGTATTGAAGCTTCAGGTATTGATAGAAGTGAACTATTCATTACGACTAAAGTATGGATTGAAAATGTTAAC
TATGAAGATACTATCAAATCAATCGAACGATCATTACAACGATTAAATTTGGACTATCTTGATCTAGTGCTTATTCACCA
ACCTTATAATGATGTATATGGCTCATGGCGTGCATTAGAAGAGTTAAAAGAAACTGGTAAAATTAAAGCAATCGGTGTAT
CTAACTTCGGCGTTGATCGAATTGTAGATTTAGGTATTCACAATCAAATTCAACCACAAGTGAACCAAATAGAAATTAAT
CCATTCCATCAACAAGAAGAACAAGTTGCAGCACTTCAACAAGAAAATGTTGTGGTAGAAGCTTGGGCACCATTTGCAGA
AGGTAAAAATCAATTATTCCAAAATCAATTGTTGCAGGCCATTGCAGATAAATATAACAAATCAATTGCACAAGTTATTT
TACGCTGTTTAGTAGAACGTGACATCGTTGTACTCGCAAAATCAGTGAATCCAGAACGTATGGCTCAAAACTTAGATATA
TTTGATTTTGAACTTACAGAAGAAGATAAACAACAAATTGCGACATTAGAAGAAAGTAATAGCCAATTTTTCTCACATGC
TGATCCAGAGATGATTAAAGCACTTACAAGCAGAGAATTAGATGTTTAA

Upstream 100 bases:

>100_bases
CAATGTTGAACGTCTTTGGGAGAGAAAGTAAACCTATGGCTTGCTATAGAGTTTGCTCCATGGTTTATAGTGAGCTTACA
AGAAAAAGGAGGTTCATTTT

Downstream 100 bases:

>100_bases
TCATAATATAACAAAGTAAATAATTTACATTACACTAACCTGAAACTTGGATGTTAAAGTCTATATTTCAGGTTATTTTT
TACTCTTAAAAAGTTAAAGG

Product: oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDTAQSYLNETEVGQGIEASGIDRSELFITTKVWIENVN
YEDTIKSIERSLQRLNLDYLDLVLIHQPYNDVYGSWRALEELKETGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRCLVERDIVVLAKSVNPERMAQNLDI
FDFELTEEDKQQIATLEESNSQFFSHADPEMIKALTSRELDV

Sequences:

>Translated_282_residues
MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDTAQSYLNETEVGQGIEASGIDRSELFITTKVWIENVN
YEDTIKSIERSLQRLNLDYLDLVLIHQPYNDVYGSWRALEELKETGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRCLVERDIVVLAKSVNPERMAQNLDI
FDFELTEEDKQQIATLEESNSQFFSHADPEMIKALTSRELDV
>Mature_282_residues
MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDTAQSYLNETEVGQGIEASGIDRSELFITTKVWIENVN
YEDTIKSIERSLQRLNLDYLDLVLIHQPYNDVYGSWRALEELKETGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRCLVERDIVVLAKSVNPERMAQNLDI
FDFELTEEDKQQIATLEESNSQFFSHADPEMIKALTSRELDV

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=283, Percent_Identity=32.1554770318021, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI5174391, Length=283, Percent_Identity=32.1554770318021, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI223468663, Length=290, Percent_Identity=33.448275862069, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI5174695, Length=299, Percent_Identity=33.4448160535117, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI310109922, Length=264, Percent_Identity=34.8484848484849, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI310109920, Length=285, Percent_Identity=32.6315789473684, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI45446745, Length=285, Percent_Identity=32.6315789473684, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI4503285, Length=285, Percent_Identity=32.6315789473684, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI5453543, Length=285, Percent_Identity=32.6315789473684, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4502049, Length=281, Percent_Identity=37.0106761565836, Blast_Score=157, Evalue=7e-39,
Organism=Homo sapiens, GI93277124, Length=296, Percent_Identity=32.7702702702703, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI24497583, Length=294, Percent_Identity=31.2925170068027, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI24497585, Length=293, Percent_Identity=30.0341296928328, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI300116273, Length=280, Percent_Identity=32.1428571428571, Blast_Score=147, Evalue=8e-36,
Organism=Homo sapiens, GI291291012, Length=282, Percent_Identity=31.5602836879433, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI300116271, Length=275, Percent_Identity=31.6363636363636, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=43.2692307692308, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=43.2692307692308, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI310109924, Length=150, Percent_Identity=30, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI87082198, Length=251, Percent_Identity=37.0517928286853, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1786400, Length=256, Percent_Identity=37.890625, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788081, Length=266, Percent_Identity=28.9473684210526, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994888, Length=181, Percent_Identity=30.939226519337, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081735, Length=215, Percent_Identity=25.5813953488372, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17537079, Length=299, Percent_Identity=29.7658862876254, Blast_Score=157, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17561298, Length=274, Percent_Identity=29.5620437956204, Blast_Score=157, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17550248, Length=298, Percent_Identity=35.5704697986577, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17564128, Length=304, Percent_Identity=30.9210526315789, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17566692, Length=275, Percent_Identity=33.4545454545455, Blast_Score=150, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17552492, Length=274, Percent_Identity=32.1167883211679, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71998625, Length=266, Percent_Identity=28.5714285714286, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17537077, Length=284, Percent_Identity=31.3380281690141, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17538386, Length=263, Percent_Identity=33.4600760456274, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17537075, Length=284, Percent_Identity=30.6338028169014, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17561300, Length=272, Percent_Identity=31.25, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17562292, Length=280, Percent_Identity=30.7142857142857, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320576, Length=286, Percent_Identity=37.7622377622378, Blast_Score=192, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6324694, Length=281, Percent_Identity=34.8754448398576, Blast_Score=171, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6322556, Length=256, Percent_Identity=35.546875, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321896, Length=303, Percent_Identity=30.6930693069307, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319625, Length=291, Percent_Identity=29.8969072164948, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320079, Length=273, Percent_Identity=30.03663003663, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24657054, Length=285, Percent_Identity=35.4385964912281, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24644950, Length=290, Percent_Identity=35.1724137931034, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI21356425, Length=291, Percent_Identity=35.0515463917526, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI221378297, Length=310, Percent_Identity=32.9032258064516, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24663317, Length=280, Percent_Identity=31.7857142857143, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI20129731, Length=287, Percent_Identity=31.7073170731707, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24662789, Length=296, Percent_Identity=32.0945945945946, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24662785, Length=283, Percent_Identity=36.0424028268551, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45553081, Length=286, Percent_Identity=30.0699300699301, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24662781, Length=292, Percent_Identity=33.9041095890411, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281366140, Length=252, Percent_Identity=31.3492063492063, Blast_Score=118, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 32107; Mature: 32107

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDTAQSYLNETEVGQGIEA
CCCCCCCCCCCCEEEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDLVLIHQPYNDVYGSWRALE
CCCCCHHEEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHH
ELKETGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEINPFHQQEEQVAALQQENVVVE
HHHHCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCEEEE
AWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRCLVERDIVVLAKSVNPERMAQNLDI
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCCC
FDFELTEEDKQQIATLEESNSQFFSHADPEMIKALTSRELDV
EEEECCHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDTAQSYLNETEVGQGIEA
CCCCCCCCCCCCEEEECCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDLVLIHQPYNDVYGSWRALE
CCCCCHHEEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHH
ELKETGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEINPFHQQEEQVAALQQENVVVE
HHHHCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCEEEE
AWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRCLVERDIVVLAKSVNPERMAQNLDI
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCCC
FDFELTEEDKQQIATLEESNSQFFSHADPEMIKALTSRELDV
EEEECCHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA