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The map label for this gene is qor [C]

Identifier: 82751784

GI number: 82751784

Start: 2214877

End: 2215884

Strand: Reverse

Name: qor [C]

Synonym: SAB2067c

Alternate gene names: 82751784

Gene position: 2215884-2214877 (Counterclockwise)

Preceding gene: 82751785

Following gene: 82751783

Centisome position: 80.8

GC content: 33.53

Gene sequence:

>1008_bases
ATGAAAATGATAGGATTTGAAAAGTCCTTTAAATTGGAAGAAGGAAATTTATTTAAAGTATACGAACAAAGAAAGCCAAC
GCCAGAAAACGATGATATCTTAGTCAAAGTAAATAGCATTAGTTTAAATCCTGTAGATACAAAACAACGACAAATGGAAG
TCACTCAAGCACCTCGTGTACTTGGTTTTGATGCTATTGGTACTGTTGAAGCTATCGGACCAGACGTTACATTATTTAGT
CCGGGTGACGTTGTATTTTATGCTGGATCGCCAAATAGACAAGGATCAAATGCGACATATCAATTGGTGTCAGAAGCTAT
AGTTGCAAAAGCACCACACAACATAAGTGCTAATGAAGCGGTTAGTCTACCGCTAACTGGTATTACGGCATATGAAACGT
TTTTTGATACATTTAAAATTTCACATAATCCTTCAGAAAATATAGGTAAATCCGTGTTAATCATTAATGGCGCTGGGGGT
GTAGGAAGTATTGCCACTCAAATTGCTAAACGGTATGGTTTAACTGTCATTACAACAGCATCACGACAAGAAACAACAGA
GTGGTGTGAAAAGATGGGCGCAGATATCGTATTGAATCATAAAGAGGATTTAGTGCGTCAATTTAAAGAAAAAGAAATTC
CATTAGTAGATTATATATTTTGCACCTATAATACAGACTTGTATTACAATACTATGATTGAACTGATAAAACCATTAGGG
CATATTACAACTATCGTAGCTTTTAATGAAGATCAAGACTTAAATGCATTAAAATTAAAAAGTATAACATTTACACATGA
ATTTATGTTTGCGCGTCCAATTCATCGAACACCTGATATGATTAAACAACATGAGTATTTAGAAGATATTACGAAAAATA
TAGAATCAGGACATTACCAACCAACAACGACACAAGTTTTTGAGGGTTTATCACCTGAAAACTTATATCAAGCACATCAA
TTGTTGGAAAAACAATCAATGATTGGGAAATTAGTAATTAATATATAG

Upstream 100 bases:

>100_bases
ATATATTCAAATGAAAAAGGAGTCATGATGCTGCGATATAAGCACACATGGCTTCTATTTTGTATAATAAGAAATAAGTT
CATAAATGAGGTGAAACGAT

Downstream 100 bases:

>100_bases
TTTAGTAAGTAAAGTCGCCTTATGCAGAAAATACTGTATAGGGCGACTTATTTTTGTGTCTAGCGACTATTGATTCTAAA
CGGAAATATGGCAACTTTGC

Product: hypothetical protein

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKMIGFEKSFKLEEGNLFKVYEQRKPTPENDDILVKVNSISLNPVDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFS
PGDVVFYAGSPNRQGSNATYQLVSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENIGKSVLIINGAGG
VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDYIFCTYNTDLYYNTMIELIKPLG
HITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQ
LLEKQSMIGKLVINI

Sequences:

>Translated_335_residues
MKMIGFEKSFKLEEGNLFKVYEQRKPTPENDDILVKVNSISLNPVDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFS
PGDVVFYAGSPNRQGSNATYQLVSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENIGKSVLIINGAGG
VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDYIFCTYNTDLYYNTMIELIKPLG
HITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQ
LLEKQSMIGKLVINI
>Mature_335_residues
MKMIGFEKSFKLEEGNLFKVYEQRKPTPENDDILVKVNSISLNPVDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFS
PGDVVFYAGSPNRQGSNATYQLVSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENIGKSVLIINGAGG
VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDYIFCTYNTDLYYNTMIELIKPLG
HITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQ
LLEKQSMIGKLVINI

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=331, Percent_Identity=25.6797583081571, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI194239674, Length=207, Percent_Identity=30.4347826086957, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI13236495, Length=207, Percent_Identity=30.4347826086957, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI194239676, Length=180, Percent_Identity=32.2222222222222, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI22538446, Length=192, Percent_Identity=30.2083333333333, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI22538444, Length=192, Percent_Identity=30.2083333333333, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI24308257, Length=236, Percent_Identity=27.5423728813559, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI28557745, Length=193, Percent_Identity=29.0155440414508, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790485, Length=189, Percent_Identity=33.8624338624339, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507255, Length=326, Percent_Identity=24.5398773006135, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71987554, Length=351, Percent_Identity=23.6467236467236, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319520, Length=188, Percent_Identity=30.8510638297872, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324195, Length=250, Percent_Identity=27.6, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 37567; Mature: 37567

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMIGFEKSFKLEEGNLFKVYEQRKPTPENDDILVKVNSISLNPVDTKQRQMEVTQAPRV
CCCCCCCCCCEECCCCEEEEEHHCCCCCCCCCEEEEEECEECCCCCCHHHHHHHHHCCCE
LGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQLVSEAIVAKAPHNISANEA
ECCCCCCCHHHCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
VSLPLTGITAYETFFDTFKISHNPSENIGKSVLIINGAGGVGSIATQIAKRYGLTVITTA
EECCCCCHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEC
SRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDYIFCTYNTDLYYNTMIELIKPLG
CCHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHH
HITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTPDMIKQHEYLEDITKNIESGHYQ
HEEEEEEECCCCCCCEEEEEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MKMIGFEKSFKLEEGNLFKVYEQRKPTPENDDILVKVNSISLNPVDTKQRQMEVTQAPRV
CCCCCCCCCCEECCCCEEEEEHHCCCCCCCCCEEEEEECEECCCCCCHHHHHHHHHCCCE
LGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQLVSEAIVAKAPHNISANEA
ECCCCCCCHHHCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
VSLPLTGITAYETFFDTFKISHNPSENIGKSVLIINGAGGVGSIATQIAKRYGLTVITTA
EECCCCCHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEC
SRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDYIFCTYNTDLYYNTMIELIKPLG
CCHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHH
HITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTPDMIKQHEYLEDITKNIESGHYQ
HEEEEEEECCCCCCCEEEEEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]