Click here to switch to the map view.

The map label for this gene is opuD

Identifier: 82751783

GI number: 82751783

Start: 2213008

End: 2214570

Strand: Reverse

Name: opuD

Synonym: SAB2066c

Alternate gene names: 82751783

Gene position: 2214570-2213008 (Counterclockwise)

Preceding gene: 82751784

Following gene: 82751782

Centisome position: 80.75

GC content: 35.7

Gene sequence:

>1563_bases
ATGAAAAATAAATCAGTAAGTCTAGTGTTTTGGATATCGTTAGTGATTTGTACGATATTTGTTGCTTTTGGTGCAATATT
TCCGAAACAGTTAGAAAAACTCACGCAAAACATTACTACATTTATTGCGCTACATTTTTCTTGGTATTATTTATTGCTCG
TTTTAGTTATTTTATTTGTCTGTGTGTATATTTTATTTTCAAGATACGCAAACATTACATTAGGTGAAGAAGGTGAAGAC
CCAGAATTTTCATTACCATCATGGTTTGCCATGTTATTTAGTGCTGGTATGGGAATAGGACTTGTGTTCTGGACAACGGC
AGAACCAATCAGTCATGCCTTCAAATTAACGCCTATTCATAAAGCTGGCACGCAATCTGCAATTAATGATGCAATGCAGT
TTTCATTTTTCCATTGGGGTATTCATGCATGGGCAGTCTATGGTATTGTCGCGCTTGTGTTTGCATATTTTAGCTTTCAT
AAAGGATATCCTGGCTTAGTCAGCGCTACATTGACGCCTTTATTAGGTGAAAAAGCAATGCGTGGCCCTTTAGGTGGTGC
CATTGATGTATTGGCAGTCATAGCTACAGTCACAGGCGTTGCTGCAACATTAGGTTTCGGTGCATTGCAAATAAACGAAG
GTTTACATTTTTTATTTAATGTACCGAGTAACTTCACGATGCAAGTAATCTTGATTGTCATTGCAACCATATTATTTACT
TGGTCAGCATGGTCAGGTATCGATAAAGGTATCAAAACGTTGAGTAATATCAACATGTTACTTGCTTTTGTAGTACTTAT
TGGTCTTTTTATTGTTGGGCCAACGCTTTACATTTTAAATACATTTACGAATGGCCTAGGTAATTATATTGCAAATTTCT
TTAGTATGAGTTTACGCATTCCGTCTGGCGGTCAAAAATTCCAATGGTTACAAAATTGGACAATCTTTTACTGGGCATGG
TGGATATCTTGGGCACCATTTGTAGGTATATTTATTGCACGTGTGTCAAAAGGACGTACGATTAAAGAATTTATTCTAGG
TGTACTTTTTGTCCCGGCGTTAGTTTGCTTTATATTCTTTGCGGTATTCGGCGCGTCAGCAATTTACTTACAAGATAATC
ATATTGCCGATATTGCTAAAGTTGCGACAGAAACAGCAACTTTTGCAACGCTACAACATTATCCGCTAGGTTTTGTATTA
AGTTTAATTACTTTACTAGTTATTATGATTTTCTTTGTTACATCAGCGGATTCGGCAACTTATGTTTTAGGTATGTTGAG
TTCAAGAGGTGATATTAATCCGAAGTCATTTGTCAAAGTGAGTTGGGGTATCATTATGGCATTATTTGCAATCATCATGA
TTTACACAGGTGGTACTCAAGCGATTCAAAATTTATTAATCATTGCTGCGTTACCATTTTCCGTGGTCATTATAGCGATG
ATATGGTCGCTTTTAAAATCTTTGAGTGAGGAGAAACCGAGAAATAGTAATAAGGTTTTAATTAAACATCGTGACCCAGA
TATTTTAGAATATCGTTCTTCAAAACATATTGACGAAAATTAA

Upstream 100 bases:

>100_bases
AGATGAAGCATTATTAATTGTGTCTATATAAAATTACAAATGATTAAAATGTTGCAGTTGTGGGTATGAACTTACCATTA
CACAGAAAGGGTTTGTATTT

Downstream 100 bases:

>100_bases
TTAACAATTAGGTTTCATTAATATGAACTAGGGAATATATTACTAAATGGCACTTCATCTTATATATTAAAAATAAATTT
ATAGTTATTAATTTATTATG

Product: glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKNKSVSLVFWISLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFVCVYILFSRYANITLGEEGED
PEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAW
WISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKVATETATFATLQHYPLGFVL
SLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
IWSLLKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN

Sequences:

>Translated_520_residues
MKNKSVSLVFWISLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFVCVYILFSRYANITLGEEGED
PEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAW
WISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKVATETATFATLQHYPLGFVL
SLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
IWSLLKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN
>Mature_520_residues
MKNKSVSLVFWISLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFVCVYILFSRYANITLGEEGED
PEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAW
WISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKVATETATFATLQHYPLGFVL
SLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
IWSLLKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=520, Percent_Identity=35.7692307692308, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1788102, Length=451, Percent_Identity=32.5942350332594, Blast_Score=232, Evalue=6e-62,
Organism=Escherichia coli, GI1786224, Length=459, Percent_Identity=29.1938997821351, Blast_Score=195, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 57734; Mature: 57734

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKSVSLVFWISLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIH
HHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHEEECCCH
KAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFHKGYPGLVSATLTPLLGEKAM
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHH
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
PSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFF
CCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AVFGASAIYLQDNHIADIAKVATETATFATLQHYPLGFVLSLITLLVIMIFFVTSADSAT
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHH
YVLGMLSSRGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IWSLLKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MKNKSVSLVFWISLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIH
HHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHEEECCCH
KAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFHKGYPGLVSATLTPLLGEKAM
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFT
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHHH
WSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
PSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFF
CCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AVFGASAIYLQDNHIADIAKVATETATFATLQHYPLGFVLSLITLLVIMIFFVTSADSAT
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHH
YVLGMLSSRGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAM
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IWSLLKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]