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The map label for this gene is prfA

Identifier: 82751719

GI number: 82751719

Start: 2132461

End: 2133537

Strand: Reverse

Name: prfA

Synonym: SAB2002c

Alternate gene names: 82751719

Gene position: 2133537-2132461 (Counterclockwise)

Preceding gene: 82751720

Following gene: 82751718

Centisome position: 77.79

GC content: 35.19

Gene sequence:

>1077_bases
GTGTTTGATCAATTAGATATTGTAGAAGAAAGATACGAACAGTTAAATGAACTGTTAAGTGACCCAGATGTTGTAAATGA
TTCAGATAAATTACGTAAATATTCTAAAGAGCAAGCTGATTTACAAAAAACTGTAGATGTTTATCGTAACTATAAAGCTA
AAAAAGAAGAATTAGCTGATATTGAAGAAATGTTAAGTGAAACTGATGATAAAGAAGAAGTAGAAATGTTAAAAGAGGAA
AGTAGCGGTATTAAAGCTGAACTTCCAAATCTTGAAGAAGAACTTAAAATATTATTGATTCCTAAAGATCCTAATGATGA
CAAAGACGTTATTGTAGAAATAAGAGCAGCAGCTGGTGGTGATGAGGCTGCGATTTTTGCTGGTGATTTAATGCGTATGT
ATTCAAAGTATGCTGAATCACAAGGATTCAAAACTGAAATAGTAGAAGCGTCTGAAAGTGACCATGGTGGTTACAAAGAA
ATTAGTTTCTCAGTTTCTGGTAATGGTGCGTATAGTAAATTGAAATTTGAAAATGGTGCGCACCGCGTTCAACGTGTGCC
TGAAACAGAATCAGGTGGACGTATTCATACTTCAACAGCTACAGTGGCAGTTTTACCAGAAGTTGAAGATGTAGAAATTG
AAATTAGAAATGAAGATTTAAAAATCGACACGTATCGTTCAAGTGGTGCAGGTGGTCAGCACGTAAACACAACTGACTCT
GCAGTACGTATTACCCATTTACCAACGGGTGTCATTGCAACATCTTCTGAGAAGTCTCAAATTCAAAACCGTGAAAAAGC
AATGAAAGTGTTAAAAGCACGTTTATACGATATGAAAGTTCAAGAAGAACAACAAAAGTATGCGTCACAACGTAAATCAG
CAGTCGGTACTGGTGATCGTTCAGAACGTATTCGAACTTATAATTATCCACAAAGCCGTGTAACAGACCACCGTATAGGT
CTAACGCTTCAAAAATTAGGGCAAATTATGGAAGGCCATTTAGAAGAAATTATAGATGCACTGACTTTATCAGAGCAGAC
AGATAAATTGAAAGAACTTAATAATGGTGAATTATAA

Upstream 100 bases:

>100_bases
TGATCCGATTATCTTAGTAGGTGCCAATGAAAGTTATGAGCCACGTTGTCGTGCGCACCATATCGTAGCACCTAGTGATA
ATAATAAGGAGGAATTATAA

Downstream 100 bases:

>100_bases
AGAAAAGTTAGATGAAGCAATTCATTTAACACAACAAAAAGGGTTTGAACAAACACGAGCTGAATGGTTAATGTTAGATG
TATTTCAATGGACGCGTACG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKTVDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEE
SSGIKAELPNLEEELKILLIPKDPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNEDLKIDTYRSSGAGGQHVNTTDS
AVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLYDMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIG
LTLQKLGQIMEGHLEEIIDALTLSEQTDKLKELNNGEL

Sequences:

>Translated_358_residues
MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKTVDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEE
SSGIKAELPNLEEELKILLIPKDPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNEDLKIDTYRSSGAGGQHVNTTDS
AVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLYDMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIG
LTLQKLGQIMEGHLEEIIDALTLSEQTDKLKELNNGEL
>Mature_358_residues
MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKTVDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEE
SSGIKAELPNLEEELKILLIPKDPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNEDLKIDTYRSSGAGGQHVNTTDS
AVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLYDMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIG
LTLQKLGQIMEGHLEEIIDALTLSEQTDKLKELNNGEL

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=46.5753424657534, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI34577120, Length=353, Percent_Identity=37.3937677053824, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI166795305, Length=236, Percent_Identity=41.1016949152542, Blast_Score=182, Evalue=3e-46,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=49.7191011235955, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=39.4736842105263, Blast_Score=231, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17542784, Length=303, Percent_Identity=36.963696369637, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=330, Percent_Identity=40, Blast_Score=229, Evalue=4e-61,
Organism=Drosophila melanogaster, GI19921226, Length=335, Percent_Identity=40, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40323; Mature: 40323

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKTVDVYRNYKAKKEELAD
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IEEMLSETDDKEEVEMLKEESSGIKAELPNLEEELKILLIPKDPNDDKDVIVEIRAAAGG
HHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCC
DEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKEISFSVSGNGAYSKLKFENGA
CCHHHHHHHHHHHHHHHHHCCCCCEEHEECCCCCCCCCEEEEEEECCCCCEEEEEECCCH
HRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNEDLKIDTYRSSGAGGQHVNTTDS
HHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCC
AVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLYDMKVQEEQQKYASQRKSAVGTGDR
EEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHLEEIIDALTLSEQTDKLKELNNGEL
CCCCEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKTVDVYRNYKAKKEELAD
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IEEMLSETDDKEEVEMLKEESSGIKAELPNLEEELKILLIPKDPNDDKDVIVEIRAAAGG
HHHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCC
DEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKEISFSVSGNGAYSKLKFENGA
CCHHHHHHHHHHHHHHHHHCCCCCEEHEECCCCCCCCCEEEEEEECCCCCEEEEEECCCH
HRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNEDLKIDTYRSSGAGGQHVNTTDS
HHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCC
AVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLYDMKVQEEQQKYASQRKSAVGTGDR
EEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHLEEIIDALTLSEQTDKLKELNNGEL
CCCCEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA