Click here to switch to the map view.

The map label for this gene is leuB

Identifier: 82751661

GI number: 82751661

Start: 2070293

End: 2071339

Strand: Direct

Name: leuB

Synonym: SAB1943

Alternate gene names: 82751661

Gene position: 2070293-2071339 (Clockwise)

Preceding gene: 82751660

Following gene: 82751662

Centisome position: 75.49

GC content: 35.63

Gene sequence:

>1047_bases
ATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGATCTCTATCATTGCTTGAAAT
TATAAGTAATAAATATAACTTTAATTATCAAATAGAGCACCACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGC
CTTTAACTGAGAAAACGTTAAATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACA
GATCCTAACAATCGACCAGAACAAGGATTATTAAAATTACGTAAATCATTAAATTTATTTGCAAATATACGCCCCACTAC
CGTTTTCAAAGGCGCTAGTTCTTTATCACCTTTAAAGCAAGAACGCGTTGAAGGTACAGATTTAGTTATAGTCCGTGAAT
TGACAAGTGGTATTTATTTTGGAGAACCTAGACATTTTAATAATCACGAGGCCTTAGATTCTCTTACTTATACAAGAGAA
GAAATAGAACGCATTGTTCACGTAGCATTTAAATTGGCCGCTTCAAGACGAGGAAAACTAACATCAGTTGATAAAGAAAA
TGTATTAGCTTCTAGTAAATTGTGGCGCAAAGTCGTAAATGAAGTGAGTCAATTATATCCAGAAGTAACAGTAAATCACT
TACTTGTTGATGCTTGTAGTATGCATTTAATCACAAATCCAAAACAATTTGACGTCATCGTTTGTGAAAACTTATTTGGC
GATATTTTAAGCGATGAAGCTTCAGTGATTCCTGGTTCACTTGGCTTATCACCTTCTGCTAGTTTTAGTAACGATGGTCC
AAGATTGTATGAGCCTATTCATGGATCAGCACCAGATATTGCAGGTAAAAACGTTGCCAATCCATTTGGAATGATTCTAT
CTTTAGCGATGTGTTTACGTGAAAGCTTAAATCAACCAGATGCTGCAGATGAATTAGAACAACATATTTATAACATGATT
GAAAATGGGCAAACGACAGCAGATTTAGGCGGCAAACTGAATACTACTGATATTTTCGAAATTCTATCTCAAAAATTGAA
TCACTAA

Upstream 100 bases:

>100_bases
TTGGTATTGATCATGATATTTTACAAGCCTCTTGTAAAGCATACGTAGAAGCACATGCTAAATTTGCAGCTGAAAATGTT
GAAAAGGTAGGTAATTAATT

Downstream 100 bases:

>100_bases
GGGGGAGATGTAAATGGGTCAAACATTATTTGACAAGGTGTGGAACAGACATGTGTTATACGGAAAATTGGGCGAACCGC
AACTATTATACATTGATTTA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWT
DPNNRPEQGLLKLRKSLNLFANIRPTTVFKGASSLSPLKQERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFG
DILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMI
ENGQTTADLGGKLNTTDIFEILSQKLNH

Sequences:

>Translated_348_residues
MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWT
DPNNRPEQGLLKLRKSLNLFANIRPTTVFKGASSLSPLKQERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFG
DILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMI
ENGQTTADLGGKLNTTDIFEILSQKLNH
>Mature_347_residues
TYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTD
PNNRPEQGLLKLRKSLNLFANIRPTTVFKGASSLSPLKQERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREE
IERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFGD
ILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIE
NGQTTADLGGKLNTTDIFEILSQKLNH

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=350, Percent_Identity=31.1428571428571, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI28178821, Length=351, Percent_Identity=29.6296296296296, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI28178816, Length=359, Percent_Identity=29.2479108635098, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4758582, Length=342, Percent_Identity=28.3625730994152, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178838, Length=318, Percent_Identity=29.2452830188679, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=33.5051546391753, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI87081683, Length=353, Percent_Identity=47.0254957507082, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1788101, Length=363, Percent_Identity=34.7107438016529, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1787381, Length=374, Percent_Identity=25.9358288770053, Blast_Score=102, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71986051, Length=335, Percent_Identity=30.7462686567164, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI25144293, Length=351, Percent_Identity=30.1994301994302, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=352, Percent_Identity=29.8295454545455, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=350, Percent_Identity=29.1428571428571, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=357, Percent_Identity=45.9383753501401, Blast_Score=292, Evalue=6e-80,
Organism=Saccharomyces cerevisiae, GI6322097, Length=368, Percent_Identity=33.9673913043478, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=353, Percent_Identity=34.2776203966006, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324291, Length=358, Percent_Identity=30.4469273743017, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24643268, Length=360, Percent_Identity=33.0555555555556, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24643270, Length=361, Percent_Identity=33.5180055401662, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24661184, Length=357, Percent_Identity=29.6918767507003, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281362242, Length=340, Percent_Identity=29.1176470588235, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648872, Length=340, Percent_Identity=29.1176470588235, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078635, Length=364, Percent_Identity=28.5714285714286, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078633, Length=364, Percent_Identity=28.5714285714286, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24650122, Length=364, Percent_Identity=28.5714285714286, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078637, Length=364, Percent_Identity=28.5714285714286, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078639, Length=361, Percent_Identity=28.808864265928, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI20130355, Length=350, Percent_Identity=22.5714285714286, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38237; Mature: 38106

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTL
CCEEEEEECCCCCCHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCHHHHHHH
NACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFANIRPTTVFKGASSLSPLKQ
HHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEECCCCCCCHHHH
ERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREEIERIVHVAFKLAASRRGKL
HHCCCCCEEEEEEHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
DILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLR
HHHCCCCHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ESLNQPDAADELEQHIYNMIENGQTTADLGGKLNTTDIFEILSQKLNH
HHCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTL
CEEEEEECCCCCCHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCHHHHHHH
NACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFANIRPTTVFKGASSLSPLKQ
HHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEECCCCCCCHHHH
ERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREEIERIVHVAFKLAASRRGKL
HHCCCCCEEEEEEHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
DILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLR
HHHCCCCHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ESLNQPDAADELEQHIYNMIENGQTTADLGGKLNTTDIFEILSQKLNH
HHCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA