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The map label for this gene is nadE

Identifier: 82751570

GI number: 82751570

Start: 1984759

End: 1985580

Strand: Reverse

Name: nadE

Synonym: SAB1849c

Alternate gene names: 82751570

Gene position: 1985580-1984759 (Counterclockwise)

Preceding gene: 82751571

Following gene: 82751569

Centisome position: 72.4

GC content: 33.7

Gene sequence:

>822_bases
ATGAGTAAATTACAAGACGTTATTGTACAAGAAATGAAAGTGAAAAAGCGTATCGATAGTGCTGAAGAAATTATGGAATT
AAAGCAATTTATAAAAAATTATGTACAATCACATTCATTTATAAAATCTTTAGTGTTAGGTATTTCAGGAGGACAAGATT
CTACATTAGTTGGAAAATTAGTACAAATGTCTGTTAACGAATTACGTGAAGAAGGCATTGATTGTACGTTTATTGCAGTT
AAATTACCTTATGGAGTTCAAAAAGATGCCGATGAAGTAGAGCAAGCTTTGCGATTCATTGAACCAGACGAAATAGTTAC
TGTAAATATTAAACCTGCAGTTGATCAAAGTGTGCAATCATTAAAAGAAGCCGGTATTGTTCTTACAGATTTCCAAAAAG
GAAATGAAAAAGCGCGTGAACGTATGAAAGTACAATTTTCAATTGCTTCAAACCGACAAGGTATTGTAGTAGGAACAGAT
CATTCAGCTGAAAATATAACTGGTTTTTATACGAAGTATGGTGATGGTGCTGCAGATATCGCACCTATATTTGGTTTGAA
TAAACGACAAGGTCGTCAATTATTAGCGTATCTTGGTGCGCCAAAGGAATTATATGAAAAAACGCCAACTGCTGATTTAG
AAGATGATAAACCACAACTTCCAGATGAAGATGCATTAGGTGTAACTTATGAGGCGATTGATAATTATTTAGAAGGTAAG
CCAGTTTCGCCAGAAGAACAAAAAGTAATTGAAAATCATTATATACGAAATGCACACAAACGTGAACTTGCATATACAAG
ATACACGTGGCCAAAATCCTAA

Upstream 100 bases:

>100_bases
CCACAAGAATATCCAGTCGATTTAAGCAAGGCATGTTGGGATAATAAACATAAACGTATTTTTGAAGTTGCGGAACACGT
TAAGGAGATGGAAGAAGATA

Downstream 100 bases:

>100_bases
TTTAATTTTTCTTCTAACGTGTGACTTAAATTAAATATGAGTTAGAATTAATAACGTTAAACCACATTCAGCTAGGCTAC
TTCAGTATATAAATTGAAAG

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSLVLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAV
KLPYGVQKDADEVEQALRFIEPDEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKPQLPDEDALGVTYEAIDNYLEGK
PVSPEEQKVIENHYIRNAHKRELAYTRYTWPKS

Sequences:

>Translated_273_residues
MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSLVLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAV
KLPYGVQKDADEVEQALRFIEPDEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKPQLPDEDALGVTYEAIDNYLEGK
PVSPEEQKVIENHYIRNAHKRELAYTRYTWPKS
>Mature_272_residues
SKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSLVLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVK
LPYGVQKDADEVEQALRFIEPDEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTDH
SAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKPQLPDEDALGVTYEAIDNYLEGKP
VSPEEQKVIENHYIRNAHKRELAYTRYTWPKS

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=273, Percent_Identity=58.6080586080586, Blast_Score=320, Evalue=8e-89,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30683; Mature: 30552

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSLVLGISGGQDSTLVGKL
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEPDEIVTVNIKPAVDQSVQS
HHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHH
LKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTDHSAENITGFYTKYGDGAADI
HHHCCCEEEECCCCCHHHHHHHEEEEEEECCCCEEEEECCCCCCCCEEHEEECCCCCHHH
APIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKPQLPDEDALGVTYEAIDNYLEGK
HHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCC
PVSPEEQKVIENHYIRNAHKRELAYTRYTWPKS
CCCCHHHHHHHHHHHHCHHHHHHHHEECCCCCC
>Mature Secondary Structure 
SKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSLVLGISGGQDSTLVGKL
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEPDEIVTVNIKPAVDQSVQS
HHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHH
LKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTDHSAENITGFYTKYGDGAADI
HHHCCCEEEECCCCCHHHHHHHEEEEEEECCCCEEEEECCCCCCCCEEHEEECCCCCHHH
APIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKPQLPDEDALGVTYEAIDNYLEGK
HHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCC
PVSPEEQKVIENHYIRNAHKRELAYTRYTWPKS
CCCCHHHHHHHHHHHHCHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA