Click here to switch to the map view.

The map label for this gene is lig

Identifier: 82751560

GI number: 82751560

Start: 1974291

End: 1976294

Strand: Reverse

Name: lig

Synonym: SAB1839c

Alternate gene names: 82751560

Gene position: 1976294-1974291 (Counterclockwise)

Preceding gene: 82751561

Following gene: 82751559

Centisome position: 72.06

GC content: 34.18

Gene sequence:

>2004_bases
ATGGCTGATTTATCGTCTCGTGTGAACGAGTTACATGATTTACTAAATCAATACAGTTATGAATACTATGTAGAGGATAA
TCCATCTGTACCAGATAGTGAATATGACAAATTACTTCATGAACTGATTAAAATAGAAGAGGAGCATCCTGAGTATAAGA
CTGTAGATTCTCCAACAGTTAGAGTTGGCGGTGAAGCCCAAGCCTCTTTCAATAAAGTCAACCATGACACGCCAATGTTA
AGTTTAGGCAATGCATTTAATGAGGATGATTTGAGAAAATTCGACCAACGCATACGTGAACAAATTGGCAACGTTGAATA
TATGTGCGAATTAAAAATTGATGGCTTAGCAGTATCATTGAAATATGTTGATGGATACTTCGTTCAAGGTTTAACACGTG
GTGATGGAACAACAGGTGAAGATATTACCGAAAATTTAAAAACAATTCATGCGATACCTTTGAAAATGAAAGAACCATTA
AATGTAGAAGTTCGTGGTGAAGCATATATGCCGAGACGTTCATTTTTACGATTAAATGAAGAAAAAGAAAAAAATGATGA
GCAGTTATTTGCAAATCCAAGAAACGCTGCTGCGGGATCATTAAGACAGTTAGATTCTAAATTAACGGCAAAACGAAAGC
TAAGCGTATTTATATATAGTGTCAATGATTTCACTGATTTCAATGCGCGTTCGCAAAGTGAAGCATTAGATGAGTTAGAT
AAATTAGGTTTTACAACGAATAAAAATAGAGCGCGTGTAAATAATATCGATGGTGTTTTAGAGTATATTGAAAAATGGAC
AAGCCAAAGAGAGTCATTACCTTATGATATTGATGGGATTGTTATTAAGGTTAATGATTTAGATCAACAGGATGAGATGG
GATTCACACAAAAATCTCCTAGATGGGCCATTGCTTATAAATTTCCAGCTGAGGAAGTAGTAACTAAATTATTAGATATT
GAATTAAGCATTGGACGTACAGGTGTAGTCACACCTACTGCTATTTTAGAACCAGTAAAAGTAGCTGGTACAACTGTATC
AAGAGCATCTTTGCACAATGAAGATTTAATTCATGACAGAGATATTCGAATTGGTGATAGTGTTGTAGTGAAAAAAGCAG
GTGACATCATACCTGAAGTTGTACGTAGTATTCCAGAACGTAGACCTGAGGGTGCTGTCACATATCATATGCCAACCCAT
TGTCCAAGTTGTGGACATGAATTAGTACGTATTGAAGGCGAAGTAGCACTTCGTTGCATTAATCCAAAATGCCAAGCACA
ACTTGTTGAAGGATTGATTCACTTTGTATCAAGACAAGCCATGAATATTGATGGACTAGGCACTAAAATTATTCAACAGC
TTTATCAAAGCGAATTAATTAAAGATGTTGCTGATATTTTCTATTTAACAGAAGAAGATTTATTACCTTTAGATAGAATG
GGGCAGAAAAAAGTTGATAATTTATTAGCTGCCATTCAACAAGCTAAGGACAACTCTTTAGAAAATTTATTATTTGGTCT
AGGTATTAGGCATTTAGGTGTTAAAGCGAGCCAAGTGTTAGCAGAAAAATATGAAACGATAGATCGATTACTAACGGTAA
CTGAAGCGGAATTAGTAGAAATTCATGATATAGGTGATAAAGTAGCACAATCTGTAGTTACTTATTTAGAAAATGAAGAT
ATTCGTGCTTTAATTCAAAAATTAAAAGATAAACATGTTAATATGATTTATAAAGGTATCAAAACATCAGATATTGAAGG
ACATCCTGAATTTAGTGGTAAAACGATAGTACTAACTGGTAAGCTACATCAAATGACACGCAATGAAGCATCTAAATGGC
TTGCATCACAAGGTGCTAAAGTTACAAGTAGCGTTACTAAAAATACAGATGTCGTTATTGCTGGTGAAGATGCAGGTTCA
AAATTAACAAAAGCACAAAGTTTAGGTATTGAAATTTGGACAGAGCAACAATTTGTAGACAAGCAAAATGAATTAAATAG
TTAG

Upstream 100 bases:

>100_bases
GAAAAATGGCTCAATCGAACTAGATATTATCTTTAAATCACAAGGGCCAAAACGTTTGTTAGCGCAATTTGCACCAATTG
AAAAAAAGGAGGATTAAGGG

Downstream 100 bases:

>100_bases
AGGGGTATGTCGATGAAGCGTACATTAGTATTATTGATTACAGCTATCTTTATACTCGCTGCTTGTGGTAACCATAAGGA
TGACCAGGCTGGAAAAGATA

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 667; Mature: 666

Protein sequence:

>667_residues
MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIKIEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPML
SLGNAFNEDDLRKFDQRIREQIGNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFIYSVNDFTDFNARSQSEALDELD
KLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYDIDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDI
ELSIGRTGVVTPTAILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEGAVTYHMPTH
CPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKIIQQLYQSELIKDVADIFYLTEEDLLPLDRM
GQKKVDNLLAAIQQAKDNSLENLLFGLGIRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENED
IRALIQKLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSSVTKNTDVVIAGEDAGS
KLTKAQSLGIEIWTEQQFVDKQNELNS

Sequences:

>Translated_667_residues
MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIKIEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPML
SLGNAFNEDDLRKFDQRIREQIGNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFIYSVNDFTDFNARSQSEALDELD
KLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYDIDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDI
ELSIGRTGVVTPTAILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEGAVTYHMPTH
CPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKIIQQLYQSELIKDVADIFYLTEEDLLPLDRM
GQKKVDNLLAAIQQAKDNSLENLLFGLGIRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENED
IRALIQKLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSSVTKNTDVVIAGEDAGS
KLTKAQSLGIEIWTEQQFVDKQNELNS
>Mature_666_residues
ADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIKIEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLS
LGNAFNEDDLRKFDQRIREQIGNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPLN
VEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFIYSVNDFTDFNARSQSEALDELDK
LGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYDIDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIE
LSIGRTGVVTPTAILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEGAVTYHMPTHC
PSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKIIQQLYQSELIKDVADIFYLTEEDLLPLDRMG
QKKVDNLLAAIQQAKDNSLENLLFGLGIRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDI
RALIQKLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSSVTKNTDVVIAGEDAGSK
LTKAQSLGIEIWTEQQFVDKQNELNS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=667, Percent_Identity=44.527736131934, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI87082305, Length=576, Percent_Identity=23.0902777777778, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75024; Mature: 74893

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIKIEEEHPEYKTVDSPTV
CCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
RVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQIGNVEYMCELKIDGLAVSL
EECCCCHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCEEEEE
KYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPLNVEVRGEAYMPRRSFLRLNE
EECCCCEEECEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHCCC
EKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFIYSVNDFTDFNARSQSEALDELD
HHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHH
KLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYDIDGIVIKVNDLDQQDEMGFTQKSP
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHCCCCCCCC
RWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTAILEPVKVAGTTVSRASLHNEDLIHDR
CEEEEEECCHHHHHHHHHHHEEECCCCCCCCHHHHHCCHHHCCCCHHHHHCCCCCCCCCC
DIRIGDSVVVKKAGDIIPEVVRSIPERRPEGAVTYHMPTHCPSCGHELVRIEGEVALRCI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHEEEECEEEEEEC
NPKCQAQLVEGLIHFVSRQAMNIDGLGTKIIQQLYQSELIKDVADIFYLTEEDLLPLDRM
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
GQKKVDNLLAAIQQAKDNSLENLLFGLGIRHLGVKASQVLAEKYETIDRLLTVTEAELVE
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IHDIGDKVAQSVVTYLENEDIRALIQKLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEC
KLHQMTRNEASKWLASQGAKVTSSVTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVD
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHCCEEEECCHHHHC
KQNELNS
CHHCCCC
>Mature Secondary Structure 
ADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIKIEEEHPEYKTVDSPTV
CCHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
RVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQIGNVEYMCELKIDGLAVSL
EECCCCHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCEEEEE
KYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPLNVEVRGEAYMPRRSFLRLNE
EECCCCEEECEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHCCC
EKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFIYSVNDFTDFNARSQSEALDELD
HHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHH
KLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYDIDGIVIKVNDLDQQDEMGFTQKSP
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHCCCCCCCC
RWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTAILEPVKVAGTTVSRASLHNEDLIHDR
CEEEEEECCHHHHHHHHHHHEEECCCCCCCCHHHHHCCHHHCCCCHHHHHCCCCCCCCCC
DIRIGDSVVVKKAGDIIPEVVRSIPERRPEGAVTYHMPTHCPSCGHELVRIEGEVALRCI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHEEEECEEEEEEC
NPKCQAQLVEGLIHFVSRQAMNIDGLGTKIIQQLYQSELIKDVADIFYLTEEDLLPLDRM
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
GQKKVDNLLAAIQQAKDNSLENLLFGLGIRHLGVKASQVLAEKYETIDRLLTVTEAELVE
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IHDIGDKVAQSVVTYLENEDIRALIQKLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEC
KLHQMTRNEASKWLASQGAKVTSSVTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVD
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHCCEEEECCHHHHC
KQNELNS
CHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA