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The map label for this gene is putP

Identifier: 82751556

GI number: 82751556

Start: 1969661

End: 1971199

Strand: Direct

Name: putP

Synonym: SAB1835

Alternate gene names: 82751556

Gene position: 1969661-1971199 (Clockwise)

Preceding gene: 82751547

Following gene: 82751558

Centisome position: 71.82

GC content: 35.74

Gene sequence:

>1539_bases
ATGCTTACAATGGGGACAGCATTAAGTCAACAAGTAAATGCCAATTGGCAAACTTATATTATGATTGCCGTCTACTTTTT
GATACTAATCGTTATTGGCTTTTACGGTTACAAGCAAGCAACTGGTAACTTAAGCGAGTACATGTTAGGTGGACGTAGTA
TTGGACCGTATATTACTGCATTATCAGCTGGAGCTTCAGATATGAGTGGATGGATGATTATGGGTCTACCTGGTTCTGTC
TATGGCACTGGTCTATCAGCTATGTGGATTACAATCGGTTTAACATTAGGCGCTTATATAAATTACTTTGTTGTTGCTCC
TAGACTTCGTGTTTATACCGAATTAGCTGGAGATGCAATTACATTACCAGATTTCTTTAAAAATCGTTTAAACGATAAAA
ATAATGTGTTAAAGATTATTTCTGGATTGATTATCGTAGTATTCTTTACATTATATACACATTCTGGTTTCGTATCTGGT
GGTAAATTATTTGAAAGTGCTTTTGGATTAGATTATCATTTCGGTTTAATATTAGTTGCTTTCATTGTCATTTTCTATAC
TTTCTTTGGTGGATATTTAGCTGTATCAATTACAGATTTCTTCCAAGGTGTCATTATGTTAATTGCGATGGTTATGGTCC
CTATTGTTGCTATGATGAATTTAAACGGCTGGGGAACGTTTCATGATGTAGCAGCTATGAAACCTACAAATTTAAATTTA
TTTAAAGGGTTATCATTTATAGGAACTATCTCTCTATTTTCATGGGGATTAGGTTATTTCGGTCAACCTCATATCATTGT
AAGGTTTATGTCTATTAAATCACACAAGATGCTACCTAAAGCTAGACGTTTAGGTATTAGCTGGATGGCAGTTGGTTTAT
TAGGCGCTGTGGCTGTTGGTTTAACAGGTATTGCATTCGTACCTGCTTATCATATTAAACTAGAAGATCCTGAGACATTA
TTCATCGTGATGAGTCAAGTACTCTTCCATCCTCTTGTAGGTGGTTTCTTACTTGCTGCGATTCTAGCTGCAATTATGAG
CACGATTTCTTCACAATTACTTGTAACATCTAGTTCATTAACGGAAGACTTTTATAAATTAATTCGTGGTGAAGAAAAAG
CTAAAACGCACCAAAAGGAATTTGTTATGATTGGAAGATTATCTGTATTAGTTGTAGCAATTGTTGCCATCGCGATTGCA
TGGAATCCAAACGACACAATTCTAAACTTAGTAGGTAATGCTTGGGCCGGATTTGGTGCATCATTCAGTCCACTTGTGCT
ATTTGCACTTTACTGGAAAGGTTTGACACGTGCCGGTGCTGTAAGTGGAATGGTTTCAGGTGCCTTAGTCGTTATCGTTT
GGATTGCGTGGATTAAACCATTAGCACATATCAACGAAATATTCGGCTTATATGAAATTATTCCTGGATTTATTGTAAGT
GTAATCGTTACATATGTTGTAAGTAAACTTACTAAAAAACCTGGTGCATTTGTTGAAACTGACTTAAACAAAGTCCGCGA
CATCGTTAGAGAAAAATAA

Upstream 100 bases:

>100_bases
AAAAGAATAAATTTAATTTTCCTATTTTTCTAAACATTTATCTTTATGTATAATGTTTTCAAGTAACTAAATTATAAATT
AAATAAAGGGAGTGTTTATC

Downstream 100 bases:

>100_bases
AATTTTATGTCATATCTCGTGCATGAATGAAATATTAAATGCACCCTAATAATCGATTGTTGAAATAACCTGAAAATTAT
TTCTCAATCGATTTTTCTAT

Product: high affinity proline permease

Products: NA

Alternate protein names: Proline permease [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MLTMGTALSQQVNANWQTYIMIAVYFLILIVIGFYGYKQATGNLSEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSV
YGTGLSAMWITIGLTLGAYINYFVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAMMNLNGWGTFHDVAAMKPTNLNL
FKGLSFIGTISLFSWGLGYFGQPHIIVRFMSIKSHKMLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETL
FIVMSQVLFHPLVGGFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVVAIVAIAIA
WNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVS
VIVTYVVSKLTKKPGAFVETDLNKVRDIVREK

Sequences:

>Translated_512_residues
MLTMGTALSQQVNANWQTYIMIAVYFLILIVIGFYGYKQATGNLSEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSV
YGTGLSAMWITIGLTLGAYINYFVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAMMNLNGWGTFHDVAAMKPTNLNL
FKGLSFIGTISLFSWGLGYFGQPHIIVRFMSIKSHKMLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETL
FIVMSQVLFHPLVGGFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVVAIVAIAIA
WNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVS
VIVTYVVSKLTKKPGAFVETDLNKVRDIVREK
>Mature_512_residues
MLTMGTALSQQVNANWQTYIMIAVYFLILIVIGFYGYKQATGNLSEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSV
YGTGLSAMWITIGLTLGAYINYFVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAMMNLNGWGTFHDVAAMKPTNLNL
FKGLSFIGTISLFSWGLGYFGQPHIIVRFMSIKSHKMLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETL
FIVMSQVLFHPLVGGFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVVAIVAIAIA
WNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVS
VIVTYVVSKLTKKPGAFVETDLNKVRDIVREK

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. Since most S.aureus strains are L- proline auxotrophs, this transporter may aid the bacterial persistence during an infection of tissues with low proline concentrations (By similarity) [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=512, Percent_Identity=73.6328125, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI1787251, Length=494, Percent_Identity=50, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI87082237, Length=475, Percent_Identity=29.2631578947368, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1790503, Length=480, Percent_Identity=25.4166666666667, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1790113, Length=393, Percent_Identity=24.1730279898219, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24645928, Length=374, Percent_Identity=25.668449197861, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24640370, Length=401, Percent_Identity=25.9351620947631, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648033, Length=401, Percent_Identity=24.6882793017456, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356865, Length=401, Percent_Identity=24.6882793017456, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221459584, Length=356, Percent_Identity=23.0337078651685, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 55933; Mature: 55933

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTMGTALSQQVNANWQTYIMIAVYFLILIVIGFYGYKQATGNLSEYMLGGRSIGPYITA
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCCCCCHHHHH
LSAGASDMSGWMIMGLPGSVYGTGLSAMWITIGLTLGAYINYFVVAPRLRVYTELAGDAI
HHCCCCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEE
TLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSGGKLFESAFGLDYHFGLILVA
ECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHH
FIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAMMNLNGWGTFHDVAAMKPTNLNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHH
FKGLSFIGTISLFSWGLGYFGQPHIIVRFMSIKSHKMLPKARRLGISWMAVGLLGAVAVG
HHHHHHHHHHHHHHHCCCCCCCCHHEEEEHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHH
LTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVGGFLLAAILAAIMSTISSQLLVTSSSL
HHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
TEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVVAIVAIAIAWNPNDTILNLVGNAWAGFGA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCC
SFSPLVLFALYWKGLTRAGAVSGMVSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIVTYVVSKLTKKPGAFVETDLNKVRDIVREK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTMGTALSQQVNANWQTYIMIAVYFLILIVIGFYGYKQATGNLSEYMLGGRSIGPYITA
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCCCCCHHHHH
LSAGASDMSGWMIMGLPGSVYGTGLSAMWITIGLTLGAYINYFVVAPRLRVYTELAGDAI
HHCCCCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEE
TLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSGGKLFESAFGLDYHFGLILVA
ECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHH
FIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAMMNLNGWGTFHDVAAMKPTNLNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHH
FKGLSFIGTISLFSWGLGYFGQPHIIVRFMSIKSHKMLPKARRLGISWMAVGLLGAVAVG
HHHHHHHHHHHHHHHCCCCCCCCHHEEEEHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHH
LTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVGGFLLAAILAAIMSTISSQLLVTSSSL
HHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
TEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVVAIVAIAIAWNPNDTILNLVGNAWAGFGA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCC
SFSPLVLFALYWKGLTRAGAVSGMVSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIVTYVVSKLTKKPGAFVETDLNKVRDIVREK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA