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The map label for this gene is dnaB

Identifier: 82751273

GI number: 82751273

Start: 1664915

End: 1666315

Strand: Reverse

Name: dnaB

Synonym: SAB1544c

Alternate gene names: 82751273

Gene position: 1666315-1664915 (Counterclockwise)

Preceding gene: 82751274

Following gene: 82751272

Centisome position: 60.76

GC content: 32.76

Gene sequence:

>1401_bases
ATGGGACGACAAGCCTTCGAATTCGGCTTAAGACCAAAGGATCAATTCAAAGTCATGCAACACTTTGATTTAAATACGAA
TCATTTAGAGGTATTGAATCGACTGTATACACCGCTAATCGGAACACAAGCAGTGGGTTTATACCATTTTATGACTCAAT
TTGTTAAAGAAAGTCATAATGAAACATTGATTCTTTCTCATTATATTTTTATGAATGAACTTAAAATTAATTTACTAGAG
TTTAGACAACAAATGGACTTACTTGAAGCGATTGGATTGTTAAAGGCGTTTGTCAAACATGATGAACAAGAAACACAATT
TGTTTACCAATTAATTCAACCGCCTTCAGCACATTTATTTTTTAATGATCCAATGTTGTCAATCTTTTTATATAGTGAAG
TAGAGCATCGTCGATTTCATGAGTTGAAGAAATACTTTGAGTATCAACAAATTGATTTGAGTGAATTTAAACAGGTAACG
AGACAATTTACTGATGTTTTTAAAGTGCCGTCTACAAAAATAGATATCGATACAAGTGATATTCCGATTAATGAACCGTA
CCAAGGTATCGATTTATCAAATGAATCTTTTGACTTTGTAATGTTGAGACAAATGTTAGGTAAACATTTTATTAGTCAAG
ACATTGTTACCAAAGATGCAAAGCGTTTAATTACACAACTCGCGACTTTATATGGTTTAACAGCAGATGGCATGAAGCAT
GTTATTTTAAATTCAATTACAAGTGGACAACAGTTATCCTTTGAAGAAATGCGTAAACAAGCACGCTCGTATTACTTAAT
GGAACACGAAAATCAAATGCCTAAATTACAGGTGAAATCTCCTGCTACATCATCTTCGACGGCCAAATCTTCAGAAGTGA
ATCCAAAGCCACAAAGTGATGAATGGTTTGAATTACTTGAACAAACGAGTCCAATTGATATGTTAGCTTCTTGGTCTGAA
TCAGAACCAACTATATCGCAAAAGACAATGGTTGAAGAATTAATAGAACGTGAAAAAATGTCTTTTGGCGTCATTAATAT
TTTGTTGCAATTTGTCATGTTAAAAGAAGATATGAAACTTCCTAAAGCGTATATTTTGGAAATAGCATCAAATTGGAAGA
AAAAAGGTATTAAGACAGCTAAAGAAGCATATAATTACGCTAAAAAAGTTAATCAACCTAAAAATGAGGGTTCTAGCGGA
AATTACCAAAAGCGTGGTTCATACTATGGGCAGCGTAATCGTATTTCAAAAGAAAAAACACCTAAATGGTTAGAAAATAG
AGATAAACCTAGTGAAGAAGATTCGGCTAAAGATAATAGCGTAGATGATCAACAATTAGAGCAAGATCGACAAGCATTTC
TAGATAAATTATCTAAAAAATGGGAGGAGGACAGTCAATAA

Upstream 100 bases:

>100_bases
TGTTAGATTTGCATCAGTCTATAAAGAATTTAAAGATGTTGATCAATTGTTAGCATCGATGCAAGGGATTTTAAGCGAAA
ACAAACGGAGTGATGCTTAA

Downstream 100 bases:

>100_bases
TGAAGCAATTTAAAAGTATAATTAACACGTCGCAGGACTTTGAAAAAAGAATAGAAAAGATAAAAAAAGAAGTAATCAAT
GACCCAGATGTTAAGCAATT

Product: chromosome replication initiation and membrane attachment protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQAVGLYHFMTQFVKESHNETLILSHYIFMNELKINLLE
FRQQMDLLEAIGLLKAFVKHDEQETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFVMLRQMLGKHFISQDIVTKDAKRLITQLATLYGLTADGMKH
VILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKLQVKSPATSSSTAKSSEVNPKPQSDEWFELLEQTSPIDMLASWSE
SEPTISQKTMVEELIEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQPKNEGSSG
NYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDRQAFLDKLSKKWEEDSQ

Sequences:

>Translated_466_residues
MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQAVGLYHFMTQFVKESHNETLILSHYIFMNELKINLLE
FRQQMDLLEAIGLLKAFVKHDEQETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFVMLRQMLGKHFISQDIVTKDAKRLITQLATLYGLTADGMKH
VILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKLQVKSPATSSSTAKSSEVNPKPQSDEWFELLEQTSPIDMLASWSE
SEPTISQKTMVEELIEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQPKNEGSSG
NYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDRQAFLDKLSKKWEEDSQ
>Mature_465_residues
GRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQAVGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEF
RQQMDLLEAIGLLKAFVKHDEQETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVTR
QFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFVMLRQMLGKHFISQDIVTKDAKRLITQLATLYGLTADGMKHV
ILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKLQVKSPATSSSTAKSSEVNPKPQSDEWFELLEQTSPIDMLASWSES
EPTISQKTMVEELIEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQPKNEGSSGN
YQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDRQAFLDKLSKKWEEDSQ

Specific function: Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110 [H]

COG id: COG3611

COG function: function code L; Replication initiation/membrane attachment protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006343 [H]

Pfam domain/function: PF07261 DnaB_2 [H]

EC number: NA

Molecular weight: Translated: 54548; Mature: 54417

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQAVGLYHFMTQFVKESHN
CCCCHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDEQETQFVYQLIQPPSAHLF
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEE
FNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVTRQFTDVFKVPSTKIDIDTSD
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCC
IPINEPYQGIDLSNESFDFVMLRQMLGKHFISQDIVTKDAKRLITQLATLYGLTADGMKH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKLQVKSPATSSSTAKSSEVNPKPQSD
HHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
EWFELLEQTSPIDMLASWSESEPTISQKTMVEELIEREKMSFGVINILLQFVMLKEDMKL
HHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKAYILEIASNWKKKGIKTAKEAYNYAKKVNQPKNEGSSGNYQKRGSYYGQRNRISKEKT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCHHHCCCCCCC
PKWLENRDKPSEEDSAKDNSVDDQQLEQDRQAFLDKLSKKWEEDSQ
CHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQAVGLYHFMTQFVKESHN
CCCHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDEQETQFVYQLIQPPSAHLF
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEE
FNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVTRQFTDVFKVPSTKIDIDTSD
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCC
IPINEPYQGIDLSNESFDFVMLRQMLGKHFISQDIVTKDAKRLITQLATLYGLTADGMKH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKLQVKSPATSSSTAKSSEVNPKPQSD
HHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCC
EWFELLEQTSPIDMLASWSESEPTISQKTMVEELIEREKMSFGVINILLQFVMLKEDMKL
HHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKAYILEIASNWKKKGIKTAKEAYNYAKKVNQPKNEGSSGNYQKRGSYYGQRNRISKEKT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCHHHCCCCCCC
PKWLENRDKPSEEDSAKDNSVDDQQLEQDRQAFLDKLSKKWEEDSQ
CHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3027697; 3027671; 8969504; 9387221; 9384377 [H]