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The map label for this gene is queA

Identifier: 82751238

GI number: 82751238

Start: 1629802

End: 1630866

Strand: Reverse

Name: queA

Synonym: SAB1509c

Alternate gene names: 82751238

Gene position: 1630866-1629802 (Counterclockwise)

Preceding gene: 82751239

Following gene: 82751237

Centisome position: 59.47

GC content: 33.43

Gene sequence:

>1065_bases
ATGGACATTTTGCAAAGTCGAATGAGGAGAGAGAATAACGTGAATATTGAAGAATTTGACTATGACTTACCAGAATCATT
AATTGCTCAAACGCCTTTAAAAGATCGTGATCATAGTCGTTTATTAGTCATGGATAGAGAAACTGGTGAAATGAAACATT
TACATTTCAAAGATATCATTGAGTATTTTAGACCTGGTGATACATTAGTGCTTAACGATACGCGAGTAATGCCAGCTAGA
CTTTTTGGTTTAAAAGAAGAAACTGGTGCAAAAGTTGAAATGTTAATGTTAACCCAAATTGAAGGTAATGATTGGGAAGT
CTTACTGAAACCAGCTAAGCGTATTAAAGTTGGTAATAAATTGAATTTTGGTAATGGCAAAATTATAGCTGAATGCATAA
AAGAAATGGATCAAGGTGGACGCATCATGCGTTTACATTATGAAGGTATTTTACAAGAAAGATTAGATGAATTAGGGGAA
ATGCCACTGCCACCATACATCAAAGAACGTTTAGATGATCCAGATCGTTATCAAACAGTTTACGCTAAAGAAAGTGGTTC
AGCGGCAGCACCTACAGCAGGATTACATTTTACTGATGAGTTATTAACTGAAATTAAAAATAAAGGTGTTAATATCGCAT
TTGTTACATTACATGTTGGATTAGGTACGTTTAGACCTGTGAGCGTCGACGATGTGAATGACCACGAAATGCATAGTGAA
TATTATCAAATGACACAAGAAACAGCTGATTTATTAAATGATACTAAGTCCAAAGGACATCGTATTATATCAGTTGGTAC
AACTTCAACACGTACACTTGAAACAATTCGACGCGATCATGATAAATTTGTTGAAACGAGTGGCTGGACTAATATATTTA
TTTATCCAGGATTTGATTTTAAAGCAATTGATGGCCAGATTACTAATTTTCATTTACCAAAATCAACATTAGTTATGCTA
GTATCAGCATTTAGTACTCGTGAAAATGTTCTGAATGCTTATAAAACGGCAGTAAATTTAGAATATAGATTCTTTAGTTT
TGGCGATGCAATGTTAATTATATAA

Upstream 100 bases:

>100_bases
GAAGAACGTATTACAATTGAGGACGTTTATGAGCCATTTCTTATTCAGAAAGGCTTTTTAGAACGTACGCCACGTGGCAG
AAAAGCAACACCATTAGCTT

Downstream 100 bases:

>100_bases
AAAGAATGTGAGGATTTTGAATATGCCTGCAGTAACATACGAACACATTAAAACTTGTAAACAATCAGGTGCGCGTTTAG
GTATCGTGCACACACCACAC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MDILQSRMRRENNVNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLNDTRVMPAR
LFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGE
MPLPPYIKERLDDPDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSE
YYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLPKSTLVML
VSAFSTRENVLNAYKTAVNLEYRFFSFGDAMLII

Sequences:

>Translated_354_residues
MDILQSRMRRENNVNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLNDTRVMPAR
LFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGE
MPLPPYIKERLDDPDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSE
YYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLPKSTLVML
VSAFSTRENVLNAYKTAVNLEYRFFSFGDAMLII
>Mature_354_residues
MDILQSRMRRENNVNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLNDTRVMPAR
LFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGE
MPLPPYIKERLDDPDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSE
YYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLPKSTLVML
VSAFSTRENVLNAYKTAVNLEYRFFSFGDAMLII

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=45.0581395348837, Blast_Score=302, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40584; Mature: 40584

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILQSRMRRENNVNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDII
CCHHHHHHHHHCCCCHHCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHH
EYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRIKVGNK
HHHCCCCEEEEECCCCCHHHHHCCCCCCCCEEEEEEEEEECCCCEEEEECCHHHEECCCE
LNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDDPDRYQTV
EECCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHEEE
YAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSE
EEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHH
YYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDF
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCE
KAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSFGDAMLII
EECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCEEEEC
>Mature Secondary Structure
MDILQSRMRRENNVNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDII
CCHHHHHHHHHCCCCHHCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHH
EYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRIKVGNK
HHHCCCCEEEEECCCCCHHHHHCCCCCCCCEEEEEEEEEECCCCEEEEECCHHHEECCCE
LNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDDPDRYQTV
EECCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHEEE
YAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSE
EEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHH
YYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDF
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCE
KAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSFGDAMLII
EECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA