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The map label for this gene is dnaG

Identifier: 82751165

GI number: 82751165

Start: 1551890

End: 1553689

Strand: Reverse

Name: dnaG

Synonym: SAB1434c

Alternate gene names: 82751165

Gene position: 1553689-1551890 (Counterclockwise)

Preceding gene: 82751166

Following gene: 82751164

Centisome position: 56.65

GC content: 32.0

Gene sequence:

>1800_bases
TTGCGAATAGATCAATCGATCATTAATGAAATAAAAGATAAAACCGACATTTTAGACTTGGTAAGTGAATATGTAAAATT
AGAAAAGAGAGGACGCAATTATATAGGTTTGTGTCCTTTTCATGATGAAAAGACCCCTTCATTTACAGTTTCTGAAGATA
AACAAATCTGTCATTGTTTTGGTTGTAAAAAAGGCGGCAATGTTTTTCAATTTACTCAAGAAATTAAAGACATATCATTT
GTTGAAGCGGTTAAAGAATTAGGTGATAGAGTTAATGTTGCTGTAGATATTGAGGCAACACAATCTAACTCAAATGTTCA
AATTGCTTCTGATGATTTACAAATGATTGAAATGCATGAGTTAATACAAGAATTTTATTATTACGCTTTAACAAAGACAG
TCGAAGGCGAACAAGCATTAACATACTTACAAGAACGTGGTTTTACAGATGCGCTTATTAAAGAGCGAGGCATTGGCTTT
GCACCCGATAGCTCACATTTTTGTCATGATTTTCTTCAAAAAAAGGGTTACGATATTGAATTAGCATATGAAGCCGGATT
ATTATCACGTAACGAAGAAAATTTCAGTTATTACGATAGATTTCGAAATCGTATTATGTTTCCTTTGAAAAATGCGCAAG
GAAGAATTGTTGGATATTCAGGTCGAACATATACCGGTCAAGAACCTAAATACCTAAATAGTCCTGAAACACCTATATTT
CAAAAAAGAAAGTTGTTATATAACTTAGATAAAGCACGTAAATCAATTAGAAAATTAGATGAAATTGTATTACTAGAAGG
TTTTATGGATGTTATAAAATCTGATACTGCTGGCTTGAAAAACGTTGTTGCAACAATGGGTACACAGTTGTCAGATGAAC
ATATTACCTTTATACGAAAGTTAACATCAAATATAACATTAATGTTTGATGGGGATTTTGCGGGTAGTGAAGCAACACTT
AAAACAGGTCAACATTTGTTACAGCAAGGGCTAAATGTATTTGTTATACAATTGCCATCTGGCATGGATCCTGATGAATA
CATTGGTAAGTATGGCAACGACGCATTTACTGCTTTTGTAAAAAATGGCAAAAAGTCATTTGCACATTATAAAGTGAGTA
TATTAAAAGATGAAATTGCACATAATGACCTTTCATATGAACGTTATTTGAAAGAACTGAGTCATGACATTTCACTTATG
AAGTCATCAATTCTACAACAAAAGGCTATAAATGATGTTGCGCCATTTTTCAATGTTAGTCCTGAGCAATTAGCTAACGA
AATACAATTCAATCAAGCACCAGCCAATTATTATCCAGAAGATGAGTATGGCGGTTACATTGAACCTGAGCCAATTGGTA
TGGCACAATTTGACAATTTGAGCCGTCAAGAAAAAGCGGAGCGAGCATTTTTAAAACATTTAATGAGAGATAAAGATACA
TTTTTAAATTATTATGAAAGTGTTGATAAGGATAACTTCACAAATCAGCATTTTAAATATGTATTCGAAGTCTTACATGA
TTTTTATGCGGAAAATGATCAATATAATATCAGTGATGCTGTGCAGTATGTTAATTCAAATGAGTTGAGAGAAACACTAA
TTAGCTTAGAACAATATAGTTTGAATGATGAACCATATGAAAATGAAATTGATGATTATGTCAATGTTATTAATGAAAAC
GGACAAGAAACAATTGAGTCATTGAATCATAAATTAAGGGAAGCTACAAGGATTGGCGATGTAGAATTACAGAAATACTA
TTTACAGCAAATTGTTGCTAAGAATAAGGAACGCATGTAG

Upstream 100 bases:

>100_bases
CGATATAATCCATTATATTGAACAAAATAAATCGAAATGATTTCATTTTTGTCAAAAATTAGGTATAATAGTATAACTAA
TGCTTAATAGGTGATTTAAT

Downstream 100 bases:

>100_bases
CATGTGATTTTAAAGAATAATACGAATAATGATTATGTCAAAATGTATAAGGGTAAATGATAGTTACCGCATTTAAACAA
CACTATTGAAAAATAAATAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISF
VEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATL
KTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFVKNGKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLM
KSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDT
FLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYSLNDEPYENEIDDYVNVINEN
GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM

Sequences:

>Translated_599_residues
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISF
VEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATL
KTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFVKNGKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLM
KSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDT
FLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYSLNDEPYENEIDDYVNVINEN
GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM
>Mature_599_residues
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISF
VEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATL
KTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFVKNGKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLM
KSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDT
FLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYSLNDEPYENEIDDYVNVINEN
GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=364, Percent_Identity=37.9120879120879, Blast_Score=244, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF00772 DnaB; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 69218; Mature: 69218

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCHHHHHHH
GCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEECCHHHHHHHHH
LIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEE
LAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
EEECCCCCCCCCCCHHHHHHHHHCEECEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHH
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
LTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFV
HHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCHHHHHHH
KNGKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKAINDVAPFFNVS
HCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
FLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYS
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHC
LNDEPYENEIDDYVNVINENGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCHHHHHHH
GCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEECCHHHHHHHHH
LIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEE
LAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
EEECCCCCCCCCCCHHHHHHHHHCEECEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHH
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
LTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFV
HHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCHHHHHHH
KNGKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKAINDVAPFFNVS
HCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
FLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYS
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHC
LNDEPYENEIDDYVNVINENGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]