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The map label for this gene is odhB

Identifier: 82751004

GI number: 82751004

Start: 1383021

End: 1384289

Strand: Reverse

Name: odhB

Synonym: SAB1268c

Alternate gene names: 82751004

Gene position: 1384289-1383021 (Counterclockwise)

Preceding gene: 82751005

Following gene: 82751003

Centisome position: 50.47

GC content: 34.52

Gene sequence:

>1269_bases
ATGCCAGAGGTTAAAGTTCCAGAATTAGCAGAATCTATTACAGAAGGTACCATTGCAGAATGGTTGAAAAACGTAGGGGA
TAGCGTAGAAAAAGGTGAAGCTATTCTTGAATTAGAAACTGATAAAGTTAATGTCGAAGTTGTATCTGAAGAAGCAGGTG
TATTATCTGAACAACTTGCCAGTGAAGGCGACACTGTAGAAGTTGGACAAGCAATTGCTGTCATCGGCGAAGGTAGTGGC
AATGCTTCTAAAGAAAATAGCAACGACAATACTCCACAACAAAATGAAGAAACAAATAATAAAAAAGAAGAAACAACAAA
TAAATCGGCAGATAATGCTGAAGTCAATCAAACAAATGATTACAATCAGCAGCGTGTTAATGCTACACCTTCTGCGCGTC
GATATGCTCGTGAAAATGGTGTGAATCTTGCTGAAGTAAGTCCGAAAACAAATGATGTTGTTCGTAAAGAAGATATTGAT
AAGAAACAACAGGCACCGGCATCAACACAAACAACACAACAAGCACCTGCAAAAGAAGAGAAAAAATACAATCAATATCC
TACAAAACCAGTGATTCGTGAAAAAATGTCACGCAGAAAGAAAACAGCTGCCAAAAAATTATTAGAGGTATCTAATAATA
CAGCTATGTTAACAACATTTAACGAAGTTGACATGACAAATGTTATGGAATTGCGTAAACGTAAGAAAGAACAATTTATG
AAAGATCATGATGGTACTAAATTAGGATTTATGTCATTCTTTACTAAAGCTTCTGTAGCAGCTTTGAAAAAGTATCCAGA
AGTTAATGCAGAAATCGACGGCGACGACATGATTACGAAACAATATTATGATATTGGTGTAGCTGTTTCTACAGATGATG
GATTATTAGTACCATTCGTAAGAGATTGTGATAAAAAGAATTTTGCAGAAATCGAAGCAGAAATTGCTAATTTAGCAGTT
AAAGCACGAGAGAAAAAACTTGGCTTAGATGATATGGTTAATGGTTCATTTACGATTACAAATGGCGGTATTTTTGGATC
AATGATGAGTACGCCAATTATCAATGGTAATCAAGCTGCAATCTTAGGCATGCATTCAATTATTACAAGACCAATTGCGA
TTGATCAAGATACAATCGAAAATCGTCCAATGATGTATATTGCATTAAGCTATGATCATAGAATTATTGACGGTAAAGAA
GCAGTTGGATTCTTAAAAACAATTAAAGAATTAATTGAAAACCCAGAAGACTTATTATTAGAATCTTAA

Upstream 100 bases:

>100_bases
CAAAGGGCTGCTCCAGCTGAAGGCGATGGAGAAATTCATAAACTTGTTCAAAATAAAATTATAGAAAATGCATTAAAAAA
TAACTAGGGGGAAATAAGTC

Downstream 100 bases:

>100_bases
TCCACAACACAAAATAGTTTATTATTCCTAAAAACGGGATCAAATCAATAACAAACAGCAGTAAGATTATTTTCTAGTCG
AAATAACTTACTGCTGTACT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVNVEVVSEEAGVLSEQLASEGDTVEVGQAIAVIGEGSG
NASKENSNDNTPQQNEETNNKKEETTNKSADNAEVNQTNDYNQQRVNATPSARRYARENGVNLAEVSPKTNDVVRKEDID
KKQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLTTFNEVDMTNVMELRKRKKEQFM
KDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAV
KAREKKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYDHRIIDGKE
AVGFLKTIKELIENPEDLLLES

Sequences:

>Translated_422_residues
MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVNVEVVSEEAGVLSEQLASEGDTVEVGQAIAVIGEGSG
NASKENSNDNTPQQNEETNNKKEETTNKSADNAEVNQTNDYNQQRVNATPSARRYARENGVNLAEVSPKTNDVVRKEDID
KKQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLTTFNEVDMTNVMELRKRKKEQFM
KDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAV
KAREKKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYDHRIIDGKE
AVGFLKTIKELIENPEDLLLES
>Mature_421_residues
PEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVNVEVVSEEAGVLSEQLASEGDTVEVGQAIAVIGEGSGN
ASKENSNDNTPQQNEETNNKKEETTNKSADNAEVNQTNDYNQQRVNATPSARRYARENGVNLAEVSPKTNDVVRKEDIDK
KQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLTTFNEVDMTNVMELRKRKKEQFMK
DHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVK
AREKKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYDHRIIDGKEA
VGFLKTIKELIENPEDLLLES

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=54.1125541125541, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI31711992, Length=439, Percent_Identity=27.7904328018223, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI110671329, Length=435, Percent_Identity=25.9770114942529, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=26.6075388026608, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=26.6075388026608, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI260898739, Length=163, Percent_Identity=35.5828220858896, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786946, Length=420, Percent_Identity=43.8095238095238, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI1786305, Length=437, Percent_Identity=30.2059496567506, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=420, Percent_Identity=39.2857142857143, Blast_Score=278, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=28.3446712018141, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=434, Percent_Identity=27.4193548387097, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=201, Percent_Identity=32.8358208955224, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320352, Length=422, Percent_Identity=40.2843601895735, Blast_Score=301, Evalue=9e-83,
Organism=Saccharomyces cerevisiae, GI6324258, Length=462, Percent_Identity=26.8398268398268, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=54.2600896860987, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI18859875, Length=428, Percent_Identity=27.803738317757, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI20129315, Length=448, Percent_Identity=24.7767857142857, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24582497, Length=430, Percent_Identity=24.6511627906977, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 46706; Mature: 46575

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVNVEVVSEEAGVLSEQLA
CCCCCCHHHHHHHHCCHHHHHHHHHCCHHHCCCEEEEEECCEEEEEEECCHHHHHHHHHH
SEGDTVEVGQAIAVIGEGSGNASKENSNDNTPQQNEETNNKKEETTNKSADNAEVNQTND
CCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCC
YNQQRVNATPSARRYARENGVNLAEVSPKTNDVVRKEDIDKKQQAPASTQTTQQAPAKEE
CCHHHCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHCCCHHHCCCCCCCHHHCCCCHHH
KKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLTTFNEVDMTNVMELRKRKKEQFM
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
KDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFV
HHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEEEECCCCEEEHHH
RDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCEEHHHHHCCCCCCCCEE
ILGMHSIITRPIAIDQDTIENRPMMYIALSYDHRIIDGKEAVGFLKTIKELIENPEDLLL
EEEHHHHHHCCCCCCCHHHCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHHHC
ES
CC
>Mature Secondary Structure 
PEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVNVEVVSEEAGVLSEQLA
CCCCCHHHHHHHHCCHHHHHHHHHCCHHHCCCEEEEEECCEEEEEEECCHHHHHHHHHH
SEGDTVEVGQAIAVIGEGSGNASKENSNDNTPQQNEETNNKKEETTNKSADNAEVNQTND
CCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCC
YNQQRVNATPSARRYARENGVNLAEVSPKTNDVVRKEDIDKKQQAPASTQTTQQAPAKEE
CCHHHCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHCCCHHHCCCCCCCHHHCCCCHHH
KKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLTTFNEVDMTNVMELRKRKKEQFM
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
KDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFV
HHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEEEECCCCEEEHHH
RDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCEEHHHHHCCCCCCCCEE
ILGMHSIITRPIAIDQDTIENRPMMYIALSYDHRIIDGKEAVGFLKTIKELIENPEDLLL
EEEHHHHHHCCCCCCCHHHCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHHHC
ES
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA