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The map label for this gene is femB

Identifier: 82750966

GI number: 82750966

Start: 1346121

End: 1347380

Strand: Direct

Name: femB

Synonym: SAB1230

Alternate gene names: 82750966

Gene position: 1346121-1347380 (Clockwise)

Preceding gene: 82750965

Following gene: 82750967

Centisome position: 49.08

GC content: 31.43

Gene sequence:

>1260_bases
ATGAAATTTACAGAGTTAACTGTTACCGAATTTGACAACTTTGTACAAAATCCATCATTGGAAAGTCATTATTTCCAAGT
AAAAGAAAATATAGTTACCCGTGAGAATGATGGCTTTGAAGTAGTTTTATTAGGTATTAAAGACGACAATAACAAAGTAA
TTGCAGCAAGCCTTTTCTCTAAAATTCCTACTATGGGAAGTTATGTTTACTATTCGAATCGTGGTCCAGTAATGGATTTT
TCAGATTTAGGATTAGTTGATTATTATTTAAAAGAGTTAGATAAATATTTACAGCAACATCAATGTTTATATGTTAAATT
AGATCCGTATTGGTTATATCATCTATATGATAAAGATATCGTGCCATTTGAAGGTCGCGAGAAAAATGATGCCTTAGTAA
ACTTGTTTAAATCGCATGGTTACGAGCATCATGGCTTTACAACTGAGTATGATACATCAAGCCAAGTACGATGGATGGGC
GTATTAAACCTTGAAGGTAAAACACCTGAAACATTGAAAAAGACATTTGATAGTCAACGTAAACGTAATATTAATAAAGC
GATAAACTATGGTGTTAAAGTAAGATTCCTTGAACGTGATGAGTTCAATCTTTTCCTAGATTTATATCGCGAAACTGAAG
AGCGTGCTGGATTTGTTTCAAAAACAGATGATTATTTTTATAACTTTATTGACACATATGGAGATAAAGTATTAGTACCA
TTAGCATATATTGACCTTGATGAATATGTGTTAAAGTTGCAACAGGAATTGAATGACAAAGAAAATCGTCGTGATCAAAT
GATGGCGAAAGAAAACAAATCAGATAAACAAATGAAGAAAATTGCAGAATTAGATAAGCAAATTGATCATGATCAGCATG
AATTATTGAATGCAAGTGAATTGAGCAAAACGGACGGCTCAATTCTAAACCTTGCTTCTGGCGTTTATTTTGCAAATGCA
TATGAAGTGAATTATTTCTCTGGGGGTTCATCAGAAAAATATAATCAATTTATGGGACCATACATGATGCATTGGTTTAT
GATTAACTATTGCTTCGATAATGGTTATGATCGTTATAATTTCTATGGTTTATCAGGTGATTTTACGGAAAACAGTGAAG
ATTATGGCGTATACCGCTTTAAACGTGGATTTAATGTACAAATCGAAGAATTAATAGGGGATTTCTATAAACCAATTCAT
AAAGTGAAATATTGGTTGTTCACAACATTGGATAAATTACGTAAAAAATTAAAGAAATAG

Upstream 100 bases:

>100_bases
TGGTGACTTTATTAAACCAATTAATAAACCTGTTTACGCAGCATATACCGCACTTAAAAAAGTTAAAGACAGAATTTTTT
AGGAAGGGAATTATCAAAAC

Downstream 100 bases:

>100_bases
ATGTAAATGATGTTAGGGCTTATAGTTATTGATACTATAGGCTCTTTTTTATGCTTTTTAGCGAAATTTTAATTATTTCA
CACTAAGAAAAACTAGTATG

Product: methicillin resistance factor protein

Products: NA

Alternate protein names: Factor essential for expression of methicillin resistance B [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLLGIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDF
SDLGLVDYYLKELDKYLQQHQCLYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGFVSKTDDYFYNFIDTYGDKVLVP
LAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDKQMKKIAELDKQIDHDQHELLNASELSKTDGSILNLASGVYFANA
YEVNYFSGGSSEKYNQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELIGDFYKPIH
KVKYWLFTTLDKLRKKLKK

Sequences:

>Translated_419_residues
MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLLGIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDF
SDLGLVDYYLKELDKYLQQHQCLYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGFVSKTDDYFYNFIDTYGDKVLVP
LAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDKQMKKIAELDKQIDHDQHELLNASELSKTDGSILNLASGVYFANA
YEVNYFSGGSSEKYNQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELIGDFYKPIH
KVKYWLFTTLDKLRKKLKK
>Mature_419_residues
MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLLGIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDF
SDLGLVDYYLKELDKYLQQHQCLYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGFVSKTDDYFYNFIDTYGDKVLVP
LAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDKQMKKIAELDKQIDHDQHELLNASELSKTDGSILNLASGVYFANA
YEVNYFSGGSSEKYNQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELIGDFYKPIH
KVKYWLFTTLDKLRKKLKK

Specific function: Catalyzes the formation of the pentaglycine interpeptide bridge, which is characteristic of the S.aureus peptidoglycan. Adds glycines 4 and 5 of the pentaglycine bridge, using glycyl- tRNA(Gly) as donor. Involved in resistance to methicillin [H]

COG id: COG2348

COG function: function code V; Uncharacterized protein involved in methicillin resistance

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the femABX family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR003447 [H]

Pfam domain/function: PF02388 FemAB [H]

EC number: NA

Molecular weight: Translated: 49666; Mature: 49666

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLLGIKDDNNKVIAASLFS
CCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEHHHHHH
KIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQCLYVKLDPYWLYHLYDKDI
HCCCCCCEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCC
VPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMGVLNLEGKTPETLKKTFDSQR
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHH
KRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGFVSKTDDYFYNFIDTYGDKVLVP
HHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEE
LAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDKQMKKIAELDKQIDHDQHELLNASE
EEEECHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCHHH
LSKTDGSILNLASGVYFANAYEVNYFSGGSSEKYNQFMGPYMMHWFMINYCFDNGYDRYN
HCCCCCCHHHHHCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
FYGLSGDFTENSEDYGVYRFKRGFNVQIEELIGDFYKPIHKVKYWLFTTLDKLRKKLKK
EEECCCCCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLLGIKDDNNKVIAASLFS
CCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEHHHHHH
KIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQCLYVKLDPYWLYHLYDKDI
HCCCCCCEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCC
VPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMGVLNLEGKTPETLKKTFDSQR
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHH
KRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGFVSKTDDYFYNFIDTYGDKVLVP
HHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEE
LAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDKQMKKIAELDKQIDHDQHELLNASE
EEEECHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCHHH
LSKTDGSILNLASGVYFANAYEVNYFSGGSSEKYNQFMGPYMMHWFMINYCFDNGYDRYN
HCCCCCCHHHHHCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
FYGLSGDFTENSEDYGVYRFKRGFNVQIEELIGDFYKPIHKVKYWLFTTLDKLRKKLKK
EEECCCCCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA