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The map label for this gene is opuD

Identifier: 82750942

GI number: 82750942

Start: 1312690

End: 1314336

Strand: Direct

Name: opuD

Synonym: SAB1206

Alternate gene names: 82750942

Gene position: 1312690-1314336 (Clockwise)

Preceding gene: 82750940

Following gene: 82750943

Centisome position: 47.86

GC content: 35.94

Gene sequence:

>1647_bases
ATGAATTCTTCTTCACCAGAGAATCCAAATGGAAAGAAGTATTCACCAGTCTTCATCTATAGTGCAATTGTTGTTGCTAT
AGTCGTATTACTTGGTGCATTTTTACCTGAACAATTCAACTATTTTACCAATAATATTAAAATGTGGATTACAGAAAAGT
TAGGTTGGTATTATCTTATTCTTACTACGATTATCGTGTTCTTCTGTATATTCCTTATTTTTAGTCCTATTGGAAAACTT
AAACTAGGTAAACCAAATGACAAACCTGAGTTTAATACAATTTCATGGTTTGCTATGTTGTTTAGTGCTGGTATGGGGAT
AGGTTTGGTGTTTTATGGTGCAGCTGAACCGATGGCGCACTTTGCTACGCCACCTACAGCAGATCCCAAAACTACTGAAG
CTTATACTGAAGCTCTACGTTCAACATTTTTCCATTGGGGATTCCATGCTTGGGCTGTTTATGGTGTTGTTGCGTTAGCG
TTGGCATATTCGCAATTCCGTAAAGGTGAACCAGGTTTATTATCTAGAACTTTACGTCCTCTTTTAGGTGATAAAGTGGA
AGGACCTATTGGGATTTTTATTGACGTTTTATCTGTATTTGCGACAATCGTTGGGGTAGCCGTTTCGTTAGGTATGGGTG
CTCTACAAATTAATGGTGGTTTACATTACTTGTTCAATGTTCCAAACAATACGTTTGTACAAGCGATTATCATCATTGTT
GTTACTATCTTATTTATAGCAAGTGCATGGTCTGGATTAAGTAAAGGTATTCAATACTTAAGTAACTTGAACATTGGTTT
AGGTACTATTTTAATGGTAGCTGCTTTAATTGTTGGACCAACTGTTCTTATTTTAAATATGTTAACTAGCTCTACGGGTA
GTTTACTAAACACATTCTTGTTTAATAGTTTTGATACAGCTGCTTTAAATCCTCAAAAACGTGAATGGATGTCTTCATGG
ACACTTTATTACTGGGGTTGGTGGTTAAGTTGGAGCCCATTCGTTGGAGTGTTTATTGCACGAGTTTCAAAAGGACGTTC
AATTAGAGAGTTCATTTCTGGTGTCTTGCTAGTTCCAGCAATTGTTAGTTTTGTATGGTTTAGTGTCTTTGGTGTATTAG
GCATCGAGACAGGTAAGAAACACAAAGAAATTTTTGATATGACTCCTGAAACTCAGCTATTTGGAGTGTTTAATCATGTG
CCATTTGGCATTGTTTTATCGTTGATTGCATTATTATTAATTGCATCATTCTTTATTACATCTGCTGACTCAGCAACATT
TGTATTAGGAATGCAAACAACATTTGGTTCATTAAATCCATCTAGTATGGTAAAAGTTGTTTGGGGAATTTCACAGGCCT
TAATAGCATTTGTACTTTTATTAGCTGGTGGCGGTAACGGCGCTGAAGCTTTAAATGCGATTCAAAGTGCTGCAATTATA
AGTGCATTCCCATTCTCCTTTGTCGTCATACTCATGATGGTAAGTTTCTACAAGGATGCGAACCAGGAACGTAAATTCCT
AGGTTTAACATTGACTCCGAATAAACACCGCTTACAAGAATATATCAAGAGTCAACAAGAAGATTATGAATCTGACATTC
TTGAAAAGCGTCAGTCACGTAGAAATATAGAGAAAAAAGATAACTAA

Upstream 100 bases:

>100_bases
TTATCCTATAAAGGTGCGTGAGTTATTGAATAAAATGTTTAGATAATATTTTTATTCTACTACATAAAATTAAATATAGA
ATTGAAGGGAGTAATTAATT

Downstream 100 bases:

>100_bases
TAAAATTTAGTTAAGTATTTTAATAACAAGTAGTATGTTATTCTAGTAGCTAGAACAGATTTACTACTTGTTTGTTTTTG
TGGAAAATTGAGTATATTCA

Product: glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYFTNNIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKL
KLGKPNDKPEFNTISWFAMLFSAGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQINGGLHYLFNVPNNTFVQAIIIIV
VTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAALIVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSW
TLYYWGWWLSWSPFVGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQLFGVFNHV
PFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGISQALIAFVLLLAGGGNGAEALNAIQSAAII
SAFPFSFVVILMMVSFYKDANQERKFLGLTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN

Sequences:

>Translated_548_residues
MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYFTNNIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKL
KLGKPNDKPEFNTISWFAMLFSAGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQINGGLHYLFNVPNNTFVQAIIIIV
VTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAALIVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSW
TLYYWGWWLSWSPFVGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQLFGVFNHV
PFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGISQALIAFVLLLAGGGNGAEALNAIQSAAII
SAFPFSFVVILMMVSFYKDANQERKFLGLTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN
>Mature_548_residues
MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYFTNNIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKL
KLGKPNDKPEFNTISWFAMLFSAGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQINGGLHYLFNVPNNTFVQAIIIIV
VTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAALIVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSW
TLYYWGWWLSWSPFVGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQLFGVFNHV
PFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGISQALIAFVLLLAGGGNGAEALNAIQSAAII
SAFPFSFVVILMMVSFYKDANQERKFLGLTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=527, Percent_Identity=33.2068311195446, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1786224, Length=507, Percent_Identity=29.7830374753452, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788102, Length=469, Percent_Identity=28.3582089552239, Blast_Score=182, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 60521; Mature: 60521

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYFTNNIKMWITEKLGWYYLI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCHHHHH
LTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFSAGMGIGLVFYGAAEPMAH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHEEEECCCCHHHH
FATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALALAYSQFRKGEPGLLSRTLRP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQINGGLHYLFNVPNNTFVQAIIIIV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCHHHHHHHHHHH
VTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAALIVGPTVLILNMLTSSTGSLLNTFL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPFVGVFIARVSKGRSIREFISGVLLVPA
HCCCCCCCCCCHHHHHHHHCHHHEEHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IVSFVWFSVFGVLGIETGKKHKEIFDMTPETQLFGVFNHVPFGIVLSLIALLLIASFFIT
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SADSATFVLGMQTTFGSLNPSSMVKVVWGISQALIAFVLLLAGGGNGAEALNAIQSAAII
CCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SAFPFSFVVILMMVSFYKDANQERKFLGLTLTPNKHRLQEYIKSQQEDYESDILEKRQSR
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RNIEKKDN
CCCCCCCC
>Mature Secondary Structure
MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYFTNNIKMWITEKLGWYYLI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCHHHHH
LTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFSAGMGIGLVFYGAAEPMAH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHEEEECCCCHHHH
FATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALALAYSQFRKGEPGLLSRTLRP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQINGGLHYLFNVPNNTFVQAIIIIV
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCHHHHHHHHHHH
VTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAALIVGPTVLILNMLTSSTGSLLNTFL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
FNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPFVGVFIARVSKGRSIREFISGVLLVPA
HCCCCCCCCCCHHHHHHHHCHHHEEHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IVSFVWFSVFGVLGIETGKKHKEIFDMTPETQLFGVFNHVPFGIVLSLIALLLIASFFIT
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SADSATFVLGMQTTFGSLNPSSMVKVVWGISQALIAFVLLLAGGGNGAEALNAIQSAAII
CCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SAFPFSFVVILMMVSFYKDANQERKFLGLTLTPNKHRLQEYIKSQQEDYESDILEKRQSR
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RNIEKKDN
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]