Click here to switch to the map view.

The map label for this gene is glpK

Identifier: 82750898

GI number: 82750898

Start: 1262912

End: 1264408

Strand: Direct

Name: glpK

Synonym: SAB1161

Alternate gene names: 82750898

Gene position: 1262912-1264408 (Clockwise)

Preceding gene: 82750897

Following gene: 82750899

Centisome position: 46.05

GC content: 36.67

Gene sequence:

>1497_bases
ATGGAAAAATATATTTTATCTATAGACCAAGGAACAACAAGCTCAAGAGCGATTTTATTCAATCAAAAAGGGGAAATTGC
AGGGGTAGCACAACGTGAGTTTAAGCAATATTTTCCACAATCAGGTTGGGTTGAACATGATGCAAATGAAATTTGGACAT
CTGTGTTAGCTGTAATGACGGAAGTAATTAATGAAAATGATGTTAGAGCTGATCAAATTGCAGGTATCGGTATTACAAAC
CAACGTGAAACAACGGTTGTTTGGGACAAACATACTGGCCGCCCAATTTATCACGCAATTGTTTGGCAATCACGTCAAAC
ACAATCAATTTGTTCAGAATTAAAACAACAAGGATATGAACAAACATTTAGAGATAAGACAGGATTACTTTTAGATCCGT
ATTTTGCAGGTACAAAAGTTAAATGGATTCTAGACAATGTTGAAGGTGCACGAGAAAAAGCAGAAAATGGCGGTTTATTA
TTTGGAACGATTGATACTTGGTTAGTATGGAAATTATCAGGAAAAGCTGCGCATATTACTGATTATTCAAATGCGAGTCG
TACATTAATGTTTAATATCCATGATTTAGAATGGGACGATGAGTTATTAGAACTACTTACAGTACCTAAAAACATGTTGC
CAGAAGTTAAACCTTCAAGTGAAGTATATGGTAAGACAATTGATTACCACTTCTATGGTCAAGAAGTACCAATTGCTGGT
GTAGCTGGTGATCAACAAGCAGCATTATTTGGACAAGCTTGCTTCGAACGTGGTGACGTGAAAAACACATATGGAACTGG
TGGCTTCATGTTAATGAATACAGGTGACAAAGCGGTTAAATCTGAAAGTGGTTTATTAACAACAATTGCTTATGGTATTG
ATGGAAAAGTAAATTATGCGCTTGAAGGTTCAATCTTTGTTTCGGGTTCAGCAATCCAATGGTTACGTGATGGATTAAGA
ATGATTAATTCAGCACCACAATCAGAAAGTTATGCGACACGAGTTGACTCTACTGAGGGTGTTTATGTTGTTCCAGCTTT
TGTAGGTTTAGGAACACCATATTGGGATTCTGAAGCACGTGGTGCGATTTTCGGTTTAACACGTGGAACTGAAAAAGAGC
ACTTTATCCGTGCAACATTAGAATCATTATGTTACCAAACTCGTGACGTTATGGAAGCAATGTCAAAAGACTCTGGAATC
GATGTCCAAAGTTTACGTGTCGATGGTGGTGCAGTTAAGAATAACTTTATTATGCAGTTCCAAGCAGACATTGTTAATAC
TTCTGTTGAAAGACCTGAAATTCAAGAAACTACAGCTTTAGGTGCTGCATATTTAGCAGGTTTAGCAGTTGGATTCTGGG
AGAGTAAAGATGATATCGCTAAAAACTGGAAATTAGAAGAAAAATTCGACCCGAAAATGGATGAAGGCGAAAGAGAAAAA
TTATATAGAGGTTGGAAAAAAGCTGTTGAAGCAACACAAGTTTTCAAAACAGAATAA

Upstream 100 bases:

>100_bases
AACATGTTTGATTCATGGATTATGCTATTTTTTCGCCAAAATTTAACAGATTTTGTACAATGGGTTAGCGATTATTTTTT
TAATAAAGGAGATACTACTA

Downstream 100 bases:

>100_bases
ACTTGTAGATTAGACTTTTGTATAAACATTGTGATACAATCAATTTAAGTTAATATTTGAATCGAGAAACGAGAGATTTG
TTCGAACATGTACAATTGAA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMTEVINENDVRADQIAGIGITN
QRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGGLL
FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKPSSEVYGKTIDYHFYGQEVPIAG
VAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLR
MINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGI
DVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAYLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREK
LYRGWKKAVEATQVFKTE

Sequences:

>Translated_498_residues
MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMTEVINENDVRADQIAGIGITN
QRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGGLL
FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKPSSEVYGKTIDYHFYGQEVPIAG
VAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLR
MINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGI
DVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAYLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREK
LYRGWKKAVEATQVFKTE
>Mature_498_residues
MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMTEVINENDVRADQIAGIGITN
QRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGGLL
FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKPSSEVYGKTIDYHFYGQEVPIAG
VAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLR
MINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGI
DVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAYLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREK
LYRGWKKAVEATQVFKTE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=503, Percent_Identity=46.1232604373757, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI42794763, Length=507, Percent_Identity=45.7593688362919, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI4504007, Length=503, Percent_Identity=46.1232604373757, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI41393575, Length=496, Percent_Identity=44.9596774193548, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI88196792, Length=505, Percent_Identity=32.8712871287129, Blast_Score=322, Evalue=7e-88,
Organism=Escherichia coli, GI1790361, Length=489, Percent_Identity=62.3721881390593, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=519, Percent_Identity=23.3140655105973, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789987, Length=477, Percent_Identity=24.1090146750524, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787789, Length=508, Percent_Identity=23.6220472440945, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1789168, Length=470, Percent_Identity=21.4893617021277, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=497, Percent_Identity=40.8450704225352, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71986140, Length=515, Percent_Identity=26.6019417475728, Blast_Score=199, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI71986147, Length=294, Percent_Identity=31.9727891156463, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321755, Length=545, Percent_Identity=34.1284403669725, Blast_Score=302, Evalue=1e-82,
Organism=Drosophila melanogaster, GI17864214, Length=502, Percent_Identity=42.2310756972112, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24654573, Length=433, Percent_Identity=44.1108545034642, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655555, Length=493, Percent_Identity=38.9452332657201, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655560, Length=493, Percent_Identity=38.9452332657201, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655570, Length=493, Percent_Identity=38.9452332657201, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655574, Length=493, Percent_Identity=38.9452332657201, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655565, Length=493, Percent_Identity=38.9452332657201, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI19922058, Length=522, Percent_Identity=30.0766283524904, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24652871, Length=489, Percent_Identity=30.4703476482618, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24656367, Length=495, Percent_Identity=27.8787878787879, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI21357867, Length=506, Percent_Identity=27.0750988142292, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24656372, Length=330, Percent_Identity=30.6060606060606, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=27.2413793103448, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55610; Mature: 55610

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMT
CCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYE
HHHCCCCCCHHHEECCCCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCHH
QTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGGLLFGTIDTWLVWKLSGKAAHIT
HHHHCCCCCEECCCCCCCEEEEEEECCHHHHHHHCCCCEEEEECCEEEEEEECCCEEEEE
DYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKPSSEVYGKTIDYHFYGQEVPIAG
CCCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCHHHCCCEEEEEEECCCCCEEE
VAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYA
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHCCCCEEEEEEECCCCEEEEE
LEGSIFVSGSAIQWLRDGLRMINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEAR
EECEEEECCCHHHHHHHHHHHHHCCCCCCCHHEECCCCCCEEEEEEHHHCCCCCCCCCCC
GAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGIDVQSLRVDGGAVKNNFIMQF
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEE
QADIVNTSVERPEIQETTALGAAYLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREK
EHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCHHHH
LYRGWKKAVEATQVFKTE
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMT
CCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
EVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYE
HHHCCCCCCHHHEECCCCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCHH
QTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGGLLFGTIDTWLVWKLSGKAAHIT
HHHHCCCCCEECCCCCCCEEEEEEECCHHHHHHHCCCCEEEEECCEEEEEEECCCEEEEE
DYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKPSSEVYGKTIDYHFYGQEVPIAG
CCCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCHHHCCCEEEEEEECCCCCEEE
VAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYA
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHCCCCEEEEEEECCCCEEEEE
LEGSIFVSGSAIQWLRDGLRMINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEAR
EECEEEECCCHHHHHHHHHHHHHCCCCCCCHHEECCCCCCEEEEEEHHHCCCCCCCCCCC
GAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGIDVQSLRVDGGAVKNNFIMQF
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEE
QADIVNTSVERPEIQETTALGAAYLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREK
EHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCHHHH
LYRGWKKAVEATQVFKTE
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA