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The map label for this gene is proS

Identifier: 82750864

GI number: 82750864

Start: 1216929

End: 1218632

Strand: Direct

Name: proS

Synonym: SAB1125

Alternate gene names: 82750864

Gene position: 1216929-1218632 (Clockwise)

Preceding gene: 82750863

Following gene: 82750865

Centisome position: 44.37

GC content: 34.98

Gene sequence:

>1704_bases
ATGAAGCAATCCAAAGTTTTTATACCAACGATGCGTGACGTGCCATCAGAAGCAGAAGCACAAAGTCATCGTTTATTATT
GAAATCGGGTTTGATAAAACAAAGTACAAGTGGGATTTATAGTTATTTACCGCTAGCAACACGTGTGTTAAATAATATTA
CTGCAATTGTGCGACAAGAAATGGAACGTATCGATTCTGTTGAAATTTTAATGCCAGCGTTACAACAAGCTGAATTATGG
GAAGAATCAGGACGTTGGGGTGCATATGGTACAGAATTAATGCGTTTACAAGATAGACATGGAAGACAATTTGCATTAGG
TCCAACACATGAAGAATTAGTTACATCAATAGTAAGAAATGAATTGAAATCATACAAACAATTACCGATGACATTATTCC
AAATTCAATCTAAATTCCGTGATGAAAAGAGACCACGTTTTGGTTTACTTCGTGGTCGTGAATTTATTATGAAAGATGCG
TATTCATTCCATGCTGACGAGGCATCATTAGATCAAACGTATCAAGATATGTATCAAGCGTATAGCCGTATTTTTGAGAG
AGTTGGCATTAACGCAAGACCAGTAGTTGCAGATTCAGGTGCTATTGGCGGTAGCCATACACATGAATTTATGGCATTAA
GTGCTATCGGTGAGGATACAATCGTTTACAGTAAAGAAAGTGACTATGCTGCTAATATCGAAAAAGCAGAAGTCGTTTAC
GAACCAAATCATAAGCATACTACTGTGCAATCTTTAGAAAAAATTGAAACACCAAATGTTAAGACTGCGCAAGAATTGGC
AGACTTCTTAGGTAGACCAGTAGATGAAATCGTTAAAACGATGATTTTCAAAGTTGATGGCGAATATATTATGGTTTTAG
TGCGTGGACATCATGAAATTAATGACATTAAATTAAAATCTTATTTCGGCACAGATAATATTGAATTAGCAACACAAGAC
GAAATTGTTAATTTAGTTGGTGCAAATCCTGGTTCACTAGGTCCTGTAATTGATAAAGAGATCAAAATTTATGCAGATAA
TTTTGTGCAAGATTTAAATAATTTAGTTGTCGGGGCTAACGAAGATGGTTATCACTTAATTAATGTAAATGTAGGTAGAG
ACTTCAACGTTGATGAATATGGCGATTTCCGTTTTATTTTAGAAGGCGAAAAGTTAAGTGATAGTTCAGGCGTTGCACAT
TTTGCTGAAGGTATTGAAGTTGGTCAAGTATTCAAATTGGGTACTAAGTATTCAGAATCAATGAATGCTACATTCTTAGA
TAACCAAGGAAAAGCCCAACCTTTAATTATGGGTTGTTACGGAATTGGAATTTCTAGAACGCTAAGTGCGATTGTTGAAC
AAAATCACGATGATAATGGAATTGTTTGGCCTAAATCAGTTACTCCGTTTGATTTACATTTAATTTCTATTAATCCTAAG
AAAGATGATCAACGAGAACTAGCGGATGCACTATATGCTGAATTTAATACTAAATTTGATGTGTTGTACGATGATCGTCA
GGAACGTGCTGGTGTCAAATTTAATGATGCCGATTTAATTGGTTTACCACTGCGAATTGTTGTTGGTAAACGTGCATCGG
AAGGTATTGTAGAAGTTAAAGAACGTTTAACAGGTGATAGCGAAGAAGTCCACATTGCTGACTTAATGACTGTCATTACA
AATAAATATGATAACTTAAAATAA

Upstream 100 bases:

>100_bases
ATTATTGCTATTGGTGCCATTTTCATGGTCGTTATTATGATATTAGTAACGTGGAATGATATTCGACGATATTTCTTATA
ATTTAGGAGGATAAATAATT

Downstream 100 bases:

>100_bases
TTAAGATCGAATGAATTATAAGAGTATGAAAAAGCAGAAAGAAATCTGATGCTTATGTCCTGCTCTTATTATTTTTGATA
TAATGATTATTCGATGAAAA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLPLATRVLNNITAIVRQEMERIDSVEILMPALQQAELW
EESGRWGAYGTELMRLQDRHGRQFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGEDTIVYSKESDYAANIEKAEVVY
EPNHKHTTVQSLEKIETPNVKTAQELADFLGRPVDEIVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQD
EIVNLVGANPGSLGPVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKLSDSSGVAH
FAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSAIVEQNHDDNGIVWPKSVTPFDLHLISINPK
KDDQRELADALYAEFNTKFDVLYDDRQERAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIADLMTVIT
NKYDNLK

Sequences:

>Translated_567_residues
MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLPLATRVLNNITAIVRQEMERIDSVEILMPALQQAELW
EESGRWGAYGTELMRLQDRHGRQFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGEDTIVYSKESDYAANIEKAEVVY
EPNHKHTTVQSLEKIETPNVKTAQELADFLGRPVDEIVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQD
EIVNLVGANPGSLGPVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKLSDSSGVAH
FAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSAIVEQNHDDNGIVWPKSVTPFDLHLISINPK
KDDQRELADALYAEFNTKFDVLYDDRQERAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIADLMTVIT
NKYDNLK
>Mature_567_residues
MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLPLATRVLNNITAIVRQEMERIDSVEILMPALQQAELW
EESGRWGAYGTELMRLQDRHGRQFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGEDTIVYSKESDYAANIEKAEVVY
EPNHKHTTVQSLEKIETPNVKTAQELADFLGRPVDEIVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQD
EIVNLVGANPGSLGPVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKLSDSSGVAH
FAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSAIVEQNHDDNGIVWPKSVTPFDLHLISINPK
KDDQRELADALYAEFNTKFDVLYDDRQERAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIADLMTVIT
NKYDNLK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=230, Percent_Identity=42.6086956521739, Blast_Score=215, Evalue=1e-55,
Organism=Escherichia coli, GI1786392, Length=570, Percent_Identity=44.3859649122807, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI115532348, Length=275, Percent_Identity=35.6363636363636, Blast_Score=169, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI193203271, Length=90, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=247, Percent_Identity=32.7935222672065, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24656200, Length=248, Percent_Identity=36.2903225806452, Blast_Score=173, Evalue=4e-43,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63854; Mature: 63854

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLPLATRVLNNITAIVRQE
CCCCCEECCCHHCCCCHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MERIDSVEILMPALQQAELWEESGRWGAYGTELMRLQDRHGRQFALGPTHEELVTSIVRN
HHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHH
ELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDAYSFHADEASLDQTYQDMYQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGEDTIVYSKESDYAANIEKAEVVY
HHHHHHHHCCCCCCEEECCCCCCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCCEEEEE
EPNHKHTTVQSLEKIETPNVKTAQELADFLGRPVDEIVKTMIFKVDGEYIMVLVRGHHEI
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHEEECCCEEEEEEECCCCC
NDIKLKSYFGTDNIELATQDEIVNLVGANPGSLGPVIDKEIKIYADNFVQDLNNLVVGAN
CCEEEEEEECCCCEEEEEHHHHHHHCCCCCCCCCCCCCCHHEEEHHHHHHHHHCEEEECC
EDGYHLINVNVGRDFNVDEYGDFRFILEGEKLSDSSGVAHFAEGIEVGQVFKLGTKYSES
CCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHCCHHCCC
MNATFLDNQGKAQPLIMGCYGIGISRTLSAIVEQNHDDNGIVWPKSVTPFDLHLISINPK
CCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEEECCC
KDDQRELADALYAEFNTKFDVLYDDRQERAGVKFNDADLIGLPLRIVVGKRASEGIVEVK
CCHHHHHHHHHHHHCCCCEEEEECCCHHHCCCEECCCCEECCCEEEEECCCCCCCHHHHH
ERLTGDSEEVHIADLMTVITNKYDNLK
HHHCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLPLATRVLNNITAIVRQE
CCCCCEECCCHHCCCCHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MERIDSVEILMPALQQAELWEESGRWGAYGTELMRLQDRHGRQFALGPTHEELVTSIVRN
HHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHH
ELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDAYSFHADEASLDQTYQDMYQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGEDTIVYSKESDYAANIEKAEVVY
HHHHHHHHCCCCCCEEECCCCCCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCCEEEEE
EPNHKHTTVQSLEKIETPNVKTAQELADFLGRPVDEIVKTMIFKVDGEYIMVLVRGHHEI
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHEEECCCEEEEEEECCCCC
NDIKLKSYFGTDNIELATQDEIVNLVGANPGSLGPVIDKEIKIYADNFVQDLNNLVVGAN
CCEEEEEEECCCCEEEEEHHHHHHHCCCCCCCCCCCCCCHHEEEHHHHHHHHHCEEEECC
EDGYHLINVNVGRDFNVDEYGDFRFILEGEKLSDSSGVAHFAEGIEVGQVFKLGTKYSES
CCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHCCHHCCC
MNATFLDNQGKAQPLIMGCYGIGISRTLSAIVEQNHDDNGIVWPKSVTPFDLHLISINPK
CCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEEECCC
KDDQRELADALYAEFNTKFDVLYDDRQERAGVKFNDADLIGLPLRIVVGKRASEGIVEVK
CCHHHHHHHHHHHHCCCCEEEEECCCHHHCCCEECCCCEECCCEEEEECCCCCCCHHHHH
ERLTGDSEEVHIADLMTVITNKYDNLK
HHHCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA