Click here to switch to the map view.

The map label for this gene is thiN [H]

Identifier: 82750827

GI number: 82750827

Start: 1173729

End: 1174370

Strand: Direct

Name: thiN [H]

Synonym: SAB1087

Alternate gene names: 82750827

Gene position: 1173729-1174370 (Clockwise)

Preceding gene: 82750826

Following gene: 82750829

Centisome position: 42.8

GC content: 32.71

Gene sequence:

>642_bases
ATGCATATAAATTTATTATGTTCTGATCGACACTTGCCGCAAGATATTTGTGCCAAAAGTAATGAAGGTAAATGGGGCGG
CGTTGATAGAGGTGCTTTGATTTTATTGAAGCATCAAATTATCCCTTTTTTCTCAGTGGGAGACTTTGATTCAGTCAGTA
AAGAAGAACGCCAACTTCTAACAGAACAGTTACAAATCAAACCTGTTCAAGCTGAAAAAGCTGATACGGATTTAGCTTTA
GCGGTTGATAAAGCTGTTGCACTTGGATTTGATAGTATTACAATTTATGGTGCAACAGGCGGACGATTAGATCACTTTTT
TGGGGCAATTCAGTTATTATTGAAAAAAGCATATTATAAACATGATGTTCATATAGAAGTTATCGATCAACAAAATAAAA
TTGAATTATTGCCTAAAGGTCAACATACAGTTGAAAAAGATAAGAGTTATCAGTACATTTCATTTATACCGATGACTGAT
GATGTAGAACTTTCTCTAGCAGGTTTTAAATATAATTTAGCTAGACAAATGCTTAATATAGGTTCTACTTTAACTATTTC
AAATGAAATTGAGTCTTTGCAAGCGAAAGTAACTGTACACGATGGGTTGATTTTGCAAATTAGAAGTAAAGATTTAAATT
GA

Upstream 100 bases:

>100_bases
ATGTGTTGAAAATGGTGCTACAATGCTAGTAACAGGTTCATTTTTCTTTAAACAAGAGGATTATAAAAAAGTCACACAAG
CATTGAAAGGTTGAGTGTAT

Downstream 100 bases:

>100_bases
GTATATACAAATTTGTGAAGATTTTTAATAAAGTGCATGAGTTATCTAATAAAGTGACGTTTATAGTATAGAATTTATAG
CATAATAATAGATGAATCAC

Product: hypothetical protein

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MHINLLCSDRHLPQDICAKSNEGKWGGVDRGALILLKHQIIPFFSVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLAL
AVDKAVALGFDSITIYGATGGRLDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYQYISFIPMTD
DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSKDLN

Sequences:

>Translated_213_residues
MHINLLCSDRHLPQDICAKSNEGKWGGVDRGALILLKHQIIPFFSVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLAL
AVDKAVALGFDSITIYGATGGRLDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYQYISFIPMTD
DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSKDLN
>Mature_213_residues
MHINLLCSDRHLPQDICAKSNEGKWGGVDRGALILLKHQIIPFFSVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLAL
AVDKAVALGFDSITIYGATGGRLDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYQYISFIPMTD
DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSKDLN

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 23860; Mature: 23860

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHINLLCSDRHLPQDICAKSNEGKWGGVDRGALILLKHQIIPFFSVGDFDSVSKEERQLL
CEEEEEECCCCCCHHHHCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCCCCHHHHHHH
TEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGRLDHFFGAIQLLLKKAYYK
HHHHEEEECCCCCCCCCEEEEEHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
HDVHIEVIDQQNKIELLPKGQHTVEKDKSYQYISFIPMTDDVELSLAGFKYNLARQMLNI
CCCEEEEECCCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHC
GSTLTISNEIESLQAKVTVHDGLILQIRSKDLN
CCEEEECCHHHCEEEEEEEECCEEEEEECCCCC
>Mature Secondary Structure
MHINLLCSDRHLPQDICAKSNEGKWGGVDRGALILLKHQIIPFFSVGDFDSVSKEERQLL
CEEEEEECCCCCCHHHHCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCCCCHHHHHHH
TEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGRLDHFFGAIQLLLKKAYYK
HHHHEEEECCCCCCCCCEEEEEHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
HDVHIEVIDQQNKIELLPKGQHTVEKDKSYQYISFIPMTDDVELSLAGFKYNLARQMLNI
CCCEEEEECCCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHC
GSTLTISNEIESLQAKVTVHDGLILQIRSKDLN
CCEEEECCHHHCEEEEEEEECCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]