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The map label for this gene is pyrF

Identifier: 82750809

GI number: 82750809

Start: 1153817

End: 1154509

Strand: Direct

Name: pyrF

Synonym: SAB1068

Alternate gene names: 82750809

Gene position: 1153817-1154509 (Clockwise)

Preceding gene: 82750808

Following gene: 82750810

Centisome position: 42.07

GC content: 34.2

Gene sequence:

>693_bases
ATGAAAGATTTACCAATTATTGCATTAGATTTTGAATCAAAAGAAAAAGTAAATCGATTTTTAGATTTATTTGATGAATC
ATTATTCGTAAAAGTAGGTATGGAACTTTTTTATCAAGAAGGTCCTCAATTAATTAATGAGATAAAAGAAAGAGGCCATG
ATGTATTTTTAGATTTAAAACTGCATGATATTCCTAATACAGTTGGTAAGGCGATGGAAGGACTAGCTAAATTGAATGTT
GATCTGGTAAATGTTCATGCTGCTGGTGGCGTAAAAATGATGTCTGAGGCCATTAAAGGATTAAGAAAACATAATGAACA
TACAAAAATTATTGCAGTAACACAGCTTACGTCAACAACAGAAGACATGTTACGACACGAACAAAATATACAAACATCGA
TTGAAGAGGCCGTTTTAAATTATGCCAAGTTAGCAAATGCAACTGGTTTAGATGGCGTTGTTTGTTCACCTCTTGAAAGT
CGTATGTTGACTGAAAAGTTAGGTGCATCATTTTTAAAAGTAACACCAGGCATTAGACCTAAAGGTGCATCTCAAGATGA
CCAACACCGTATTACGACACCGGAAGAAGCAAGACAGCTTGGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAA
GTGACAATCCAGTCGAAAGTTATCATAAAATTAAAGAAAGTTGGTTAGTATAA

Upstream 100 bases:

>100_bases
ACTGTATCGGTGGGGCTGTAATTAACCATTTACTTAAAGAAGTTTATATTACAGCCTCATTATTTTAATGAATTTCTTAA
CGTAAAGGGAGACATATATG

Downstream 100 bases:

>100_bases
TGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAATGATTTATATACATGGAGTTCA
GGCATTAAATCACCGATTTA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKDLPIIALDFESKEKVNRFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNV
DLVNVHAAGGVKMMSEAIKGLRKHNEHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANATGLDGVVCSPLES
RMLTEKLGASFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIKESWLV

Sequences:

>Translated_230_residues
MKDLPIIALDFESKEKVNRFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNV
DLVNVHAAGGVKMMSEAIKGLRKHNEHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANATGLDGVVCSPLES
RMLTEKLGASFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIKESWLV
>Mature_230_residues
MKDLPIIALDFESKEKVNRFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNV
DLVNVHAAGGVKMMSEAIKGLRKHNEHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANATGLDGVVCSPLES
RMLTEKLGASFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIKESWLV

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=225, Percent_Identity=41.3333333333333, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25649; Mature: 25649

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLPIIALDFESKEKVNRFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLK
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEE
LHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLRKHNEHTKIIAVTQLTSTT
ECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHH
EDMLRHEQNIQTSIEEAVLNYAKLANATGLDGVVCSPLESRMLTEKLGASFLKVTPGIRP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHCCHHEECCCCCCC
KGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIKESWLV
CCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDLPIIALDFESKEKVNRFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLK
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEE
LHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLRKHNEHTKIIAVTQLTSTT
ECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHH
EDMLRHEQNIQTSIEEAVLNYAKLANATGLDGVVCSPLESRMLTEKLGASFLKVTPGIRP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHCCHHEECCCCCCC
KGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIKESWLV
CCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA