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The map label for this gene is pyrB

Identifier: 82750805

GI number: 82750805

Start: 1147266

End: 1148036

Strand: Direct

Name: pyrB

Synonym: SAB1064

Alternate gene names: 82750805

Gene position: 1147266-1148036 (Clockwise)

Preceding gene: 82750804

Following gene: 82750806

Centisome position: 41.83

GC content: 32.04

Gene sequence:

>771_bases
ATGAATCATTTATTATCAATGGAACATTTATCTACAGATCAAATATACAAACTTATCCAAAAGGCAAGTCAATTTAAATC
TGGTGAACGTCAACTACCAAACTTTGAAGGGAAATATGTCGCAAATTTATTCTTTGAAAATTCTACTCGAACAAAATGTA
GTTTTGAAATGGCAGAACTTAAGCTAGGGTTAAAAACGATTAGCTTTGAAACATCAGCATCATCTGTTTCAAAAGGTGAA
TCTTTATATGACACATGTAAAACTTTAGAAAGTATTGGCTGTGATTTATTAGTCATTAGACATCCGTTTAATAACTACTA
TGAAAAATTAGCGAATATTAACATTCCAATTGCGAATGCTGGTGATGGTAGTGGACAACATCCAACACAAAGTTTGCTTG
ATTTAATGACGATATATGAAGAATATGGATATTTTGAAGGCTTGAATGCATTGATTTGTGGAGACATTAAAAATTCACGT
GTCGCACGTAGTAATTACCATAGTTTAAAAGCATTAGGTGCAAACGTAATGTTTAATAGCCCAAATGCTTGGATTGATGA
TTCTTTAGAAGCACCTTATGTAAATATAGATGATGTTATAGAAACAGTAGATATAGTTATGTTATTAAGAATTCAACATG
AAAGACATGGGCTTGCAGAAGAAACTAGATTTGCAGCAGATGATTATCATCAAAAGCATGGCTTAAATGAAGTGCGCTAT
AACAAAATACAAGAACATGCTATTGTTATGCATCCGGCACATGTGATATGA

Upstream 100 bases:

>100_bases
GATGGCATTAGCTGCACTTTCAGGAATTATTTTGAACTTAATCTTACCTAAAGAGAAAAAACAAAACAATTAAGATTTAC
AAATTAAGGAGGGCGCTTTT

Downstream 100 bases:

>100_bases
GGAGTAGAAATACAAAGCGATTTAGTAGAAGCTTCAAAATCAAGAATTTTTAAGCAAATGGAAAATGGCGTTTACTTAAG
AATGGCAGTCATTGATGAAT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLFFENSTRTKCSFEMAELKLGLKTISFETSASSVSKGE
SLYDTCKTLESIGCDLLVIRHPFNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNALICGDIKNSR
VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGLAEETRFAADDYHQKHGLNEVRY
NKIQEHAIVMHPAHVI

Sequences:

>Translated_256_residues
MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLFFENSTRTKCSFEMAELKLGLKTISFETSASSVSKGE
SLYDTCKTLESIGCDLLVIRHPFNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNALICGDIKNSR
VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGLAEETRFAADDYHQKHGLNEVRY
NKIQEHAIVMHPAHVI
>Mature_256_residues
MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLFFENSTRTKCSFEMAELKLGLKTISFETSASSVSKGE
SLYDTCKTLESIGCDLLVIRHPFNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNALICGDIKNSR
VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGLAEETRFAADDYHQKHGLNEVRY
NKIQEHAIVMHPAHVI

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=271, Percent_Identity=32.4723247232472, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI2367364, Length=230, Percent_Identity=36.0869565217391, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786469, Length=197, Percent_Identity=29.4416243654822, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790703, Length=195, Percent_Identity=27.6923076923077, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=277, Percent_Identity=32.4909747292419, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6322331, Length=270, Percent_Identity=30.3703703703704, Blast_Score=105, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24642586, Length=274, Percent_Identity=34.3065693430657, Blast_Score=138, Evalue=3e-33,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 28990; Mature: 28990

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLFFENSTRTKCSFEMAEL
CCCCCCHHHCCHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEECCCCCCHHHHHHHH
KLGLKTISFETSASSVSKGESLYDTCKTLESIGCDLLVIRHPFNNYYEKLANINIPIANA
HHHHHEEEECCCHHHHHCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEECC
GDGSGQHPTQSLLDLMTIYEEYGYFEGLNALICGDIKNSRVARSNYHSLKALGANVMFNS
CCCCCCCHHHHHHHHHHHHHHHCHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCEEEECC
PNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGLAEETRFAADDYHQKHGLNEVRY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHH
NKIQEHAIVMHPAHVI
HHHHHCEEEECCHHCC
>Mature Secondary Structure
MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLFFENSTRTKCSFEMAEL
CCCCCCHHHCCHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEECCCCCCHHHHHHHH
KLGLKTISFETSASSVSKGESLYDTCKTLESIGCDLLVIRHPFNNYYEKLANINIPIANA
HHHHHEEEECCCHHHHHCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEECC
GDGSGQHPTQSLLDLMTIYEEYGYFEGLNALICGDIKNSRVARSNYHSLKALGANVMFNS
CCCCCCCHHHHHHHHHHHHHHHCHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCEEEECC
PNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGLAEETRFAADDYHQKHGLNEVRY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHH
NKIQEHAIVMHPAHVI
HHHHHCEEEECCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA