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The map label for this gene is argF [H]

Identifier: 82750776

GI number: 82750776

Start: 1112803

End: 1113804

Strand: Direct

Name: argF [H]

Synonym: SAB1033

Alternate gene names: 82750776

Gene position: 1112803-1113804 (Clockwise)

Preceding gene: 82750772

Following gene: 82750777

Centisome position: 40.58

GC content: 34.23

Gene sequence:

>1002_bases
ATGAAAAATTTACGAAACAGAAGTTTTTTAACTTTATTAGACTTTTCAAGACAAGAGGTAGAATTCTTATTAACACTCTC
CGAGGATTTAAAACGTGCTAAATATATTGGCACTGAAAAGCCTATGTTAAAAAATAAAAATATTGCACTGTTGTTTGAAA
AAGATTCTACAAGAACGCGATGTGCATTTGAAGTTGCAGCGCATGATCAAGGTGCAAATGTAACTTATTTAGGCCCAACT
GGATCACAAATGGGTAAAAAAGAAACAACTAAAGATACTGCACGTGTGCTTGGTGGAATGTATGATGGCATTGAATACCG
TGGTTTTTCACAAAGAACAGTAGAAACTTTAGCTCAATATTCAGGCGTACCAGTGTGGAATGGTTTAACTGATGAAGATC
ATCCTACTCAAGTTCTTGCTGATTTCTTAACAGCAAAAGAAGTCTTAAAAAAAGATTATGCAGATATTAACTTTACATAT
GTTGGAGATGGTCGTAATAACGTTGCAAATGCATTAATGCAAGGCGCTGCCATTATGGGTATGAACTTCCATTTAGTTTG
TCCAAAAGAATTAAATCCAACAGATGAATTATTAAATCGCTGTAAAAATATTGCCTCTGAAAATGGTGGCAACATATTAA
TCACAGATGATATTGACCAAGGTGTAAAGGGTTCGGATGTAATTTACACTGACGTTTGGGTATCAATGGGTGAACCTGAT
GAAGTATGGAAAGAACGACTCGAATTATTGAAACCGTATCAAGTGAATAAAGAAATGATGGATAAAACTGGTAATCCTAA
TGTTATTTTTGAGCATTGCTTACCATCTTTCCACAATGCTGATACTAAAATTGGACAACAAATTTTTGAAAAATATGGAA
TTCGAGAAATGGAAGTTACAGATGAAGTATTCGAAAGTAAAGCTTCAGTTGTATTCCAAGAAGCTGAGAACAGAATGCAT
ACAATAAAAGCAGTCATGGTTGCTACATTGGGTGAATTTTAG

Upstream 100 bases:

>100_bases
TTAATTATTGATAAAATATTACAAATTTTAATAGTAGGTTGTGTTTATTTTGTATGCGCTTACAATTTAGGTGTAACTAA
AATAAAAGGAGTTGTTATTA

Downstream 100 bases:

>100_bases
ATGATATAAGGAAGTGAATATGATGGCGAAAATAGTAGTAGCATTAGGTGGTAATGCTTTAGGAAAATCACCTCAAGAAC
AACTCGAACTTGTTAAAAAT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLKNKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPT
GSQMGKKETTKDTARVLGGMYDGIEYRGFSQRTVETLAQYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIASENGGNILITDDIDQGVKGSDVIYTDVWVSMGEPD
EVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPSFHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMH
TIKAVMVATLGEF

Sequences:

>Translated_333_residues
MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLKNKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPT
GSQMGKKETTKDTARVLGGMYDGIEYRGFSQRTVETLAQYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIASENGGNILITDDIDQGVKGSDVIYTDVWVSMGEPD
EVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPSFHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMH
TIKAVMVATLGEF
>Mature_333_residues
MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLKNKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPT
GSQMGKKETTKDTARVLGGMYDGIEYRGFSQRTVETLAQYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIASENGGNILITDDIDQGVKGSDVIYTDVWVSMGEPD
EVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPSFHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMH
TIKAVMVATLGEF

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=331, Percent_Identity=36.8580060422961, Blast_Score=207, Evalue=9e-54,
Organism=Escherichia coli, GI1790703, Length=332, Percent_Identity=58.433734939759, Blast_Score=409, Evalue=1e-116,
Organism=Escherichia coli, GI1786469, Length=334, Percent_Identity=58.3832335329341, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI2367364, Length=335, Percent_Identity=25.9701492537313, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322373, Length=329, Percent_Identity=28.8753799392097, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6322331, Length=228, Percent_Identity=27.1929824561404, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24642586, Length=302, Percent_Identity=23.5099337748344, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 37550; Mature: 37550

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLKNKNIALLFEKDSTRTR
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCE
CAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYDGIEYRGFSQRTVETLAQY
EEEEEEECCCCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTYVGDGRNNVANALMQGAAIMG
CCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCEEC
MNFHLVCPKELNPTDELLNRCKNIASENGGNILITDDIDQGVKGSDVIYTDVWVSMGEPD
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCHH
EVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPSFHNADTKIGQQIFEKYGIREMEVT
HHHHHHHHHHCCCCCCHHHHHCCCCCCEEHHHCCCCCCCCHHHHHHHHHHHHCCEEEHHH
DEVFESKASVVFQEAENRMHTIKAVMVATLGEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLKNKNIALLFEKDSTRTR
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCE
CAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYDGIEYRGFSQRTVETLAQY
EEEEEEECCCCCEEEEECCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTYVGDGRNNVANALMQGAAIMG
CCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCEEC
MNFHLVCPKELNPTDELLNRCKNIASENGGNILITDDIDQGVKGSDVIYTDVWVSMGEPD
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCHH
EVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPSFHNADTKIGQQIFEKYGIREMEVT
HHHHHHHHHHCCCCCCHHHHHCCCCCCEEHHHCCCCCCCCHHHHHHHHHHHHCCEEEHHH
DEVFESKASVVFQEAENRMHTIKAVMVATLGEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA