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The map label for this gene is pheT

Identifier: 82750746

GI number: 82750746

Start: 1084189

End: 1086591

Strand: Direct

Name: pheT

Synonym: SAB1003

Alternate gene names: 82750746

Gene position: 1084189-1086591 (Clockwise)

Preceding gene: 82750745

Following gene: 82750748

Centisome position: 39.53

GC content: 34.58

Gene sequence:

>2403_bases
ATGTTGATATCAAATGAATGGTTGAAAGAATATGTAACAATCGATGATTCTGTAAGTAATTTGGCAGAACGTATTACGCG
CACAGGTATTGAAGTGGATGATTTAATTGACTACACAAAAGATATCAAAAATTTAGTTGTCGGCTTCGTTAAGTCAAAAG
AGAAACATCCTGATGCAGATAAATTAAATGTTTGCCAAGTTGATATCGGAGAAGACGAACCTGTACAAATAGTATGTGGT
GCACCGAATGTTGATGCAGGACAATATGTCATTGTTGCTAAAGTAGGTGGAAGATTGCCTGGTGGTATTAAAATTAAGCG
TGCCAAATTACGCGGTGAACGTTCAGAAGGTATGATTTGTTCGTTACAAGAAATTGGTATTTCAAGTAATTATATACCGA
AAAGTTTTGAATCAGGCATTTATGTATTTAGTGAATCCCAAGTTCCAGGAACAGATGCCTTACAAGCTTTATATTTAGAT
GATCAAGTAATGGAATTTGATTTAACGCCGAATCGTGCAGATGCTTTAAGTATGATAGGTACTGCTTATGAAGTTGCAGC
ATTATATAATACAAAAATGACTAAGCCAGAGACAACATCAAATGAGCTTGAGTTATCTGCAAATGATGAACTAACCGTGA
CAATTGAAAATGAAGATAAAGTACCATATTATAGTGCACGTGTTGTTCACAACGTGACAATTGAACCCTCGCCAATTTGG
ATGCAAGCACGCTTAATAAAAGCAGGTATACGTCCTATTAATAATGTTGTCGACATTTCAAATTATGTGTTATTAGAATA
CGGTCAACCATTGCATATGTTTGATCAAGATGCGATTGGTTCACAACAAATTGTTGTTCGTCAAGCTAATGAAGGCGAAA
AAATGACAACATTAGATGATACAGAACGTGAATTATTAACGAGCGATATTGTCATTACTAATGGACAAACTCCAATTGCA
TTAGCTGGTGTTATGGGTGGCGATTTTTCAGAAGTTAAAGAACATACATCAAATATAGTTATTGAAGGTGCTATTTTTGA
TTCTGTTTCCATTCGTCATACATCAAGACGTTTAAATTTACGCAGTGAATCATCTAGTCGTTTTGAAAAAGGAATAGCTA
CTGAATTTGTAGATGAAGCAGTCGACCGTGCATGTTATTTATTACAAACTTATGCAAACGGAAAAGTGCTAAAAGATAGA
GTATCTTCAGGAGAACTTGGTGCATTTATTACACCAATCGACATCACTGCTGATAAAATTAATCGCACTATTGGATTTGA
TTTGTCACAAAATGATATTGTTACTATTTTTAATCAACTAGGGTTTGATACAGAAATAAATGATGATGTTATTACAGTGC
AAGTACCATCACGTCGTAAAGATATTACAATTAAAGAAGATTTAATTGAAGAAGTTGCACGTATATATGGATACGACGAT
ATTCCATCAACGTTACCTGTCTTCGAAAAAGTTACTAGTGGTCAGCTAACTGATCGTCAATATAAAACTAGAATGGTTAA
AGAAGTGTTAGAAGGTGCTGGATTAGACCAAGCTATTACGTATTCGTTAGTTTCTAAAGAAGATGCTACTGCATTTGCGA
TGCAACAACGTCAAACAATTGATTTATTGATGCCAATGAGTGAAGCGCATGCGTCATTACGTCAAAGTTTATTACCACAT
TTAATCGAAGCGGCATCATATAATGTGGCACGCAAAAATAAAGATGTAAAATTATTTGAAATCGGCAATGTCTTCTTTGC
GAATGGTGAGGGTGAACTACCAGATCAAGTTGAATATTTAAGTGGTATTTTAACTGGAGATTATGTAGTCAATCAATGGC
AAGGTAAGAAAGAAACGGTTGATTTCTATTTAGCAAAAGGTGTCGTGGATCGAGTATCTGAAAAGTTAAACCTTGAATTT
AGTTATCGTCGTGCTGATATTGATGGATTACATCCAGGTCGTACAGCTGAAATCTTATTAGAGAATAAAGTTGTTGGTTT
TATTGGTGAATTACATCCAACATTAGCAGCTGATAATGATTTAAAACGGACGTATGTTTTTGAGTTGAATTTTGATGCAT
TAATGGCTGTGTCGGTAGGTTACATTAATTACCAGCCAATTCCGATATTCCCAGGTATGTCTCGTGACATTGCATTAGAA
GTAGATCAAAATATTCCAGCAGCTGATTTATTATCAACGATTCATGCACACGGTGGCAATATATTAAAAGACACACTTGT
CTTTGATGTATATCAGGGTGAACATTTAGAAAAAGGTAAAAAATCAATTGCAATACGATTAAATTATTTAGACACAGAAG
AAACATTGACAGATGAACGCGTTTCAAAAGTACAAGCGGAAATTGAAGCAGCATTAATTGAACAAGGTGCTGTTATTAGA
TAA

Upstream 100 bases:

>100_bases
CAATGTTGAAATATGGTATAGAAGATATTCGTCATTTCTATACTAATGATGTGAGATTTTTAGATCAATTTAAAGCGGTA
GAAGATAGAGGTGACATGTA

Downstream 100 bases:

>100_bases
TGATTTAAACCCCATGTATAAGGATATCTGAAGTAGATTGATATCCTTAACATGGGGTTTTATTTTTGGATTCACCAATT
AGGTTCCAATTTATCTTGAT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 800; Mature: 800

Protein sequence:

>800_residues
MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKNLVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCG
APNVDAGQYVIVAKVGGRLPGGIKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLD
DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELELSANDELTVTIENEDKVPYYSARVVHNVTIEPSPIW
MQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQDAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIA
LAGVMGGDFSEVKEHTSNIVIEGAIFDSVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYANGKVLKDR
VSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDVITVQVPSRRKDITIKEDLIEEVARIYGYDD
IPSTLPVFEKVTSGQLTDRQYKTRMVKEVLEGAGLDQAITYSLVSKEDATAFAMQQRQTIDLLMPMSEAHASLRQSLLPH
LIEAASYNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYLAKGVVDRVSEKLNLEF
SYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLKRTYVFELNFDALMAVSVGYINYQPIPIFPGMSRDIALE
VDQNIPAADLLSTIHAHGGNILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAVIR

Sequences:

>Translated_800_residues
MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKNLVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCG
APNVDAGQYVIVAKVGGRLPGGIKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLD
DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELELSANDELTVTIENEDKVPYYSARVVHNVTIEPSPIW
MQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQDAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIA
LAGVMGGDFSEVKEHTSNIVIEGAIFDSVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYANGKVLKDR
VSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDVITVQVPSRRKDITIKEDLIEEVARIYGYDD
IPSTLPVFEKVTSGQLTDRQYKTRMVKEVLEGAGLDQAITYSLVSKEDATAFAMQQRQTIDLLMPMSEAHASLRQSLLPH
LIEAASYNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYLAKGVVDRVSEKLNLEF
SYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLKRTYVFELNFDALMAVSVGYINYQPIPIFPGMSRDIALE
VDQNIPAADLLSTIHAHGGNILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAVIR
>Mature_800_residues
MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKNLVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCG
APNVDAGQYVIVAKVGGRLPGGIKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLD
DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELELSANDELTVTIENEDKVPYYSARVVHNVTIEPSPIW
MQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQDAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIA
LAGVMGGDFSEVKEHTSNIVIEGAIFDSVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYANGKVLKDR
VSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDVITVQVPSRRKDITIKEDLIEEVARIYGYDD
IPSTLPVFEKVTSGQLTDRQYKTRMVKEVLEGAGLDQAITYSLVSKEDATAFAMQQRQTIDLLMPMSEAHASLRQSLLPH
LIEAASYNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYLAKGVVDRVSEKLNLEF
SYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLKRTYVFELNFDALMAVSVGYINYQPIPIFPGMSRDIALE
VDQNIPAADLLSTIHAHGGNILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAVIR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=372, Percent_Identity=22.3118279569892, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788006, Length=805, Percent_Identity=37.888198757764, Blast_Score=469, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17533403, Length=551, Percent_Identity=22.1415607985481, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355211, Length=547, Percent_Identity=23.5831809872029, Blast_Score=100, Evalue=5e-21,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 88908; Mature: 88908

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKNLVVGFVKSKEKHPDAD
CCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGGIKIKRAKLRGERSEGMIC
CCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCEE
SLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLDDQVMEFDLTPNRADALSMIG
EHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHEEEEECCEEEEEECCCCHHHHHHHHH
TAYEVAALYNTKMTKPETTSNELELSANDELTVTIENEDKVPYYSARVVHNVTIEPSPIW
HHHHHHHHHCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCEEEEEEEEEEEECCCCCH
MQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQDAIGSQQIVVRQANEGEKMTTLDD
HHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
TERELLTSDIVITNGQTPIALAGVMGGDFSEVKEHTSNIVIEGAIFDSVSIRHTSRRLNL
HHHHHHHCCEEEECCCCCEEEEEECCCCHHHHHHHHCCEEEEEEEECCEEEEEHHHHCCC
RSESSSRFEKGIATEFVDEAVDRACYLLQTYANGKVLKDRVSSGELGAFITPIDITADKI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCEEEEEEECCHHHH
NRTIGFDLSQNDIVTIFNQLGFDTEINDDVITVQVPSRRKDITIKEDLIEEVARIYGYDD
CCEECCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHCCCCC
IPSTLPVFEKVTSGQLTDRQYKTRMVKEVLEGAGLDQAITYSLVSKEDATAFAMQQRQTI
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHEEHHHCCCCHHHHHHHHCCCC
DLLMPMSEAHASLRQSLLPHLIEAASYNVARKNKDVKLFEIGNVFFANGEGELPDQVEYL
CEECCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEECCEEEECCCCCCCHHHHHH
SGILTGDYVVNQWQGKKETVDFYLAKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILL
HHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEE
ENKVVGFIGELHPTLAADNDLKRTYVFELNFDALMAVSVGYINYQPIPIFPGMSRDIALE
ECCHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEEECEEEECCEECCCCCCCCEEEE
VDQNIPAADLLSTIHAHGGNILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDER
ECCCCCHHHHHHHHHHCCCCEEEHHEEEEEECCCHHHCCCCEEEEEEEEECCHHHHHHHH
VSKVQAEIEAALIEQGAVIR
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKNLVVGFVKSKEKHPDAD
CCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGGIKIKRAKLRGERSEGMIC
CCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCEE
SLQEIGISSNYIPKSFESGIYVFSESQVPGTDALQALYLDDQVMEFDLTPNRADALSMIG
EHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHEEEEECCEEEEEECCCCHHHHHHHHH
TAYEVAALYNTKMTKPETTSNELELSANDELTVTIENEDKVPYYSARVVHNVTIEPSPIW
HHHHHHHHHCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCEEEEEEEEEEEECCCCCH
MQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQDAIGSQQIVVRQANEGEKMTTLDD
HHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
TERELLTSDIVITNGQTPIALAGVMGGDFSEVKEHTSNIVIEGAIFDSVSIRHTSRRLNL
HHHHHHHCCEEEECCCCCEEEEEECCCCHHHHHHHHCCEEEEEEEECCEEEEEHHHHCCC
RSESSSRFEKGIATEFVDEAVDRACYLLQTYANGKVLKDRVSSGELGAFITPIDITADKI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCEEEEEEECCHHHH
NRTIGFDLSQNDIVTIFNQLGFDTEINDDVITVQVPSRRKDITIKEDLIEEVARIYGYDD
CCEECCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHCCCCC
IPSTLPVFEKVTSGQLTDRQYKTRMVKEVLEGAGLDQAITYSLVSKEDATAFAMQQRQTI
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHEEHHHCCCCHHHHHHHHCCCC
DLLMPMSEAHASLRQSLLPHLIEAASYNVARKNKDVKLFEIGNVFFANGEGELPDQVEYL
CEECCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEECCEEEECCCCCCCHHHHHH
SGILTGDYVVNQWQGKKETVDFYLAKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILL
HHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEE
ENKVVGFIGELHPTLAADNDLKRTYVFELNFDALMAVSVGYINYQPIPIFPGMSRDIALE
ECCHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEEECEEEECCEECCCCCCCCEEEE
VDQNIPAADLLSTIHAHGGNILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDER
ECCCCCHHHHHHHHHHCCCCEEEHHEEEEEECCCHHHCCCCEEEEEEEEECCHHHHHHHH
VSKVQAEIEAALIEQGAVIR
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA