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The map label for this gene is purF

Identifier: 82750681

GI number: 82750681

Start: 1016323

End: 1017807

Strand: Direct

Name: purF

Synonym: SAB0937

Alternate gene names: 82750681

Gene position: 1016323-1017807 (Clockwise)

Preceding gene: 82750680

Following gene: 82750682

Centisome position: 37.06

GC content: 35.76

Gene sequence:

>1485_bases
ATGTTTAACTACTCAGGATTAAACGAAGAATGTGGCGTGTTTGGTATTTGGAATCATCCTGAAGCAGCGCAACTAACATA
TATGGGACTTCATAGTTTGCAACATCGTGGTCAAGAAGGTGCAGGTATAGTTGTTTCTAATCAAAATGAATTAAAAGGCG
AGCGAGGATTAGGCTTACTAACTGAAGCGATTAAAGATGACCAAATGGAACGATTAAAAGGATATCAACATGCAATTGGT
CACGTCCGTTATGCTACTTCAGGTAATAAAGGTATTGAAAATATTCAACCGTTTCTGTATCACTTTTATGATATGAGTGT
AGGTATTTGTCATAATGGTAACCTCATTAATGCTAAATCATTGCGTCAGAATTTAGAAAAACAAGGTGCTATCTTCCATT
CGTCTTCTGATACTGAAGTCATTATGCATTTGATACGTCGAAGTAAAGCTCCTACTTTTGAGGAAGCGTTGAAAGAAAGT
TTGCGAAAAGTTAAAGGGGGTTTTACATTTGCGATTTTAACTAAAGATGCGTTATATGGCGCAGTAGATCCAAATGCTAT
CAGACCTCTTGTTGTAGGTAAAATGAAAGATGGTAAATACATCCTTGCAAGTGAAACATGTGCAATAGATGTGTTAGGTG
CAGCATTTGTTCAAGATATTCATGCAGGTGAATATGTCGTGATTAACGATAAAGGTATTACAGTTAAATCTTATACACAT
CATACGACAACTGCAATTTCTGCGATGGAATATATTTATTTTGCTAGACCAGATTCAACAATAGCTGGTAAAAATGTCCA
TGCAGTACGTAAAGCTTCTGGTAAAAAATTAGCCCAAGAAAGCCCTGTAAATGCTGATATGGTCATCGGTGTACCCAATT
CATCGCTATCAGCTGCGAGTGGTTATGCTGAAGAAATAGGTTTGCCATATGAAATGGGACTAGTTAAAAATCAATATGTT
GCAAGAACATTTATTCAACCAACTCAAGAATTACGTGAGCAAGGTGTGAGAGTGAAGTTATCTGCGGTAAAAGATATAGT
AGATGGGAAAAATATCATTCTTGTTGATGATTCCATTGTTCGCGGTACGACAATTCGACGCATTGTGAAAATGTTAAAAG
ATTCTGGTGCAAATAAAGTGCATGTGCGTATAGCATCACCGGAATTTATGTTCCCAAGTTTTTATGGAATCGATGTTTCA
ACTACGGCAGAATTAATTTCTGCAAGCAAATCACCTGAAGAAATTAAAGATTATATTGGCGCTGATTCATTAGCATATCT
ATCTGTAGATGGGTTAATTGAATCAATTGGTTTAGATTATGACGCGCCATATAGTGGCTTATGTGTAGAAAGTTTCACTG
GAGATTATCCTGCAGGGTTATATGATTATGAAGCAAATTATAAAGCACATTTAAGTCATCGACAAAAGCAATATATTTCT
AAAAACAAACACTTTTTTGATAGCGAGGGAAATTTAAATGTCTAA

Upstream 100 bases:

>100_bases
TTTTAACAGATAGTGATAATTTCAAAGTAACAACACCATATACAGAGATTAGTGAAAATGTTTCAGATATTAAACAAATA
TGGGAAGGGGCAATTGCTCA

Downstream 100 bases:

>100_bases
AGCATATGAACAATCTGGTGTAAATATTCATGCTGGTTATGAAGCTGTAGAAAGAATGTCTAGTCATGTTAAACGTACGA
TGCGTAAAGAAGTTATCGGT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSNQNELKGERGLGLLTEAIKDDQMERLKGYQHAIG
HVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGKYILASETCAIDVLGAAFVQDIHAGEYVVINDKGITVKSYTH
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYV
ARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVS
TTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
KNKHFFDSEGNLNV

Sequences:

>Translated_494_residues
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSNQNELKGERGLGLLTEAIKDDQMERLKGYQHAIG
HVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGKYILASETCAIDVLGAAFVQDIHAGEYVVINDKGITVKSYTH
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYV
ARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVS
TTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
KNKHFFDSEGNLNV
>Mature_494_residues
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSNQNELKGERGLGLLTEAIKDDQMERLKGYQHAIG
HVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGKYILASETCAIDVLGAAFVQDIHAGEYVVINDKGITVKSYTH
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYV
ARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVS
TTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
KNKHFFDSEGNLNV

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=510, Percent_Identity=36.8627450980392, Blast_Score=332, Evalue=7e-91,
Organism=Homo sapiens, GI4826742, Length=225, Percent_Identity=29.3333333333333, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788651, Length=448, Percent_Identity=36.1607142857143, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1790167, Length=216, Percent_Identity=28.2407407407407, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=39.5397489539749, Blast_Score=327, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6323958, Length=483, Percent_Identity=34.3685300207039, Blast_Score=239, Evalue=6e-64,
Organism=Drosophila melanogaster, GI28573187, Length=497, Percent_Identity=37.2233400402414, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24659598, Length=492, Percent_Identity=36.5853658536585, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=36.6952789699571, Blast_Score=324, Evalue=7e-89,
Organism=Drosophila melanogaster, GI21357745, Length=207, Percent_Identity=29.4685990338164, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54365; Mature: 54365

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSNQNELKGERGLGLL
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCHHH
TEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKS
HHHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCEEEEEECCCEECHHH
LRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYG
HHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCHHCC
AVDPNAIRPLVVGKMKDGKYILASETCAIDVLGAAFVQDIHAGEYVVINDKGITVKSYTH
CCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCC
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAAS
HHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCHHC
GYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIV
CCHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHH
RGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVSTTAELISASKSPEEIKDYIG
CCHHHHHHHHHHHCCCCCEEEEEECCCCEECCCEECCCHHHHHHHHHCCCCHHHHHHHHC
ADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
CCCEEEEEHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHH
KNKHFFDSEGNLNV
CCCCEECCCCCCCC
>Mature Secondary Structure
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSNQNELKGERGLGLL
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCHHH
TEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKS
HHHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCEEEEEECCCEECHHH
LRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYG
HHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCHHCC
AVDPNAIRPLVVGKMKDGKYILASETCAIDVLGAAFVQDIHAGEYVVINDKGITVKSYTH
CCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCC
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAAS
HHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCHHC
GYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIV
CCHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHH
RGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVSTTAELISASKSPEEIKDYIG
CCHHHHHHHHHHHCCCCCEEEEEECCCCEECCCEECCCHHHHHHHHHCCCCHHHHHHHHC
ADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
CCCEEEEEHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHH
KNKHFFDSEGNLNV
CCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA