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The map label for this gene is murE

Identifier: 82750630

GI number: 82750630

Start: 959278

End: 960762

Strand: Direct

Name: murE

Synonym: SAB0884

Alternate gene names: 82750630

Gene position: 959278-960762 (Clockwise)

Preceding gene: 82750627

Following gene: 82750631

Centisome position: 34.98

GC content: 37.17

Gene sequence:

>1485_bases
TTGGATGCAAGTACGTTGTTTAAGAAAGTAAAAGTAAAGCGTGTATTGGGTTCTTTAGAACAACAAATAGATGATATCAC
TACTGATTCACGTACAGCGAGAGAAGGTAGCATTTTTGTCGCTTCAGTTGGATATACTGTAGACAGTCACAAGTTCTGTC
AAAGTGTAGCTGATCAAGGGTGTAAGTTGGTAGTGGTCAATAAAGAACAATCATTACCAGCTAACGTAACACAAGTGGTT
GTGCCGGACACATTAAGAGTAGCTAGTATTCTAGCACACACATTATTTGATTATCCGAGTCATCAGTTAGTGACATTTGG
TGTAACAGGTACAAATGGTAAAACTTCTATTGCGACGATGATTCATTTAATTCAAAGAAAGTTACAAAAAAATAGTGCAT
ATTTAGGAACTAATGGTTTCCAAATTAATGAAACAAAGACAAAAGGTGCAAATACGACACCAGAAACAGTTTCTTTAACT
AAGAAAATTAAAGAAGCAGTTGATGCAGGCGCTGAATCCATGACATTAGAAGTATCAAGCCATGGCTTAGTATTAGGACG
ACTGCGAGGCGTTGAATTTGACGTTGCAATATTTTCAAATTTAACACAAGACCATTTAGATTTTCATGGCACAATGGAAG
CGTACGGACACGCGAAGTCTTTATTGTTTAGTCAATTAGGTGAAGATTTGTCGAAAGAAAAGTATGTCGTGTTAAACAAT
GACGATTCATTTTCTGAGTATTTAAGAACAGTGACGCCTTATGAAGTATTTAGTTATGGAATTGATGAGGAAGCCCAATT
TATGGCTGAAAATATTCAAGAATCTTTACAAGGTGTCAGCTTTGATTTTGTAACGCCTTTTGGAACTTACCCAGTAAAAT
CGCCTTATGTTGGTAAGTTTAATATTTCTAATATTATGGCGGCAATGATTGCGGTGTGGAGTAAAGGTACATCTTTAGAA
ACGATTATTAAAGCTGTTGAAAATTTAGAACCTGTTGAAGGGCGATTAGAAGTTTTAGATCCTTCGTTACCTATTGATTT
AATTATCGATTATGCACATACAGCTGATGGTATGAACAAATTAATCGATGCAGTACAGCCTTTTGTAAAGCAAAAGTTGA
TATTTTTAGTTGGTATGGCAGGCGAACGTGATTTGACTAAAACGCCTGAAATGGGGCGAGTTGCCTGTCGTGCAGATTAT
GTCATTTTCACACCGGATAATCCGGCAAATGATGACCCGAAAATGTTAACAGCAGAATTAGCCAAAGGTGCAACACATCA
AAACTATATTGAATTTGATGATCGTGCAGAAGGGATAAAACATGCAATTGACATAGCTGAGCCTGGGGATACTGTCGTTT
TAGCATCAAAAGGAAGAGAACCATATCAAATCATGCCAGGGCATATTAAGGTGCCACATCGAGATGATTTAATTGGCCTT
GAAGCAGCTTACAAAAAGTTCGGTGGTGGCCCTGTTGGTCAATAA

Upstream 100 bases:

>100_bases
GAAAATGCTGATTAGTAGGATATAACAGTGAAATTATAAATTTATTAACATCAACAAAACGTGTATAATAAACATATTGT
AGAAAAAGGAGCGGTTCAGT

Downstream 100 bases:

>100_bases
AAGACTTATTGATGAAGGTAAAACTATTGATGTTTATTTATTCGAAGCATTAAATGACCAGATAATCATTGCTATACCAG
ATTGGTTTTGGTCATATCAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--L-lysine ligase

Products: NA

Alternate protein names: L-lysine-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQSVADQGCKLVVVNKEQSLPANVTQVV
VPDTLRVASILAHTLFDYPSHQLVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
DDSFSEYLRTVTPYEVFSYGIDEEAQFMAENIQESLQGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLE
TIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADY
VIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
EAAYKKFGGGPVGQ

Sequences:

>Translated_494_residues
MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQSVADQGCKLVVVNKEQSLPANVTQVV
VPDTLRVASILAHTLFDYPSHQLVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
DDSFSEYLRTVTPYEVFSYGIDEEAQFMAENIQESLQGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLE
TIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADY
VIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
EAAYKKFGGGPVGQ
>Mature_494_residues
MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQSVADQGCKLVVVNKEQSLPANVTQVV
VPDTLRVASILAHTLFDYPSHQLVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
DDSFSEYLRTVTPYEVFSYGIDEEAQFMAENIQESLQGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLE
TIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADY
VIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
EAAYKKFGGGPVGQ

Specific function: Catalyzes the addition of L-lysine to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=462, Percent_Identity=30.7359307359307, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1786274, Length=318, Percent_Identity=27.0440251572327, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.7 [H]

Molecular weight: Translated: 54133; Mature: 54133

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQSVADQG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
CKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLFDYPSHQLVTFGVTGTNGKTSIATM
CEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
IHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLTKKIKEAVDAGAESMTLEVSS
HHHHHHHHHCCCCEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
HGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
CCEEEEEECCCEEEHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
DDSFSEYLRTVTPYEVFSYGIDEEAQFMAENIQESLQGVSFDFVTPFGTYPVKSPYVGKF
CCCHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
NISNIMAAMIAVWSKGTSLETIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNK
CHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCHHHEEECHHHHHHHHH
LIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADYVIFTPDNPANDDPKMLTAEL
HHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEECEEEECCCCCCCCCHHHHHHHH
AKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
HCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCEECCCEEECCCCCCCCHH
EAAYKKFGGGPVGQ
HHHHHHHCCCCCCC
>Mature Secondary Structure
MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQSVADQG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
CKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLFDYPSHQLVTFGVTGTNGKTSIATM
CEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
IHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLTKKIKEAVDAGAESMTLEVSS
HHHHHHHHHCCCCEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
HGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
CCEEEEEECCCEEEHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
DDSFSEYLRTVTPYEVFSYGIDEEAQFMAENIQESLQGVSFDFVTPFGTYPVKSPYVGKF
CCCHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCC
NISNIMAAMIAVWSKGTSLETIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNK
CHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCHHHEEECHHHHHHHHH
LIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADYVIFTPDNPANDDPKMLTAEL
HHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEECEEEECCCCCCCCCHHHHHHHH
AKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
HCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCEECCCEEECCCCCCCCHH
EAAYKKFGGGPVGQ
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA