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The map label for this gene is ksgA

Identifier: 82750200

GI number: 82750200

Start: 489342

End: 490253

Strand: Direct

Name: ksgA

Synonym: SAB0442

Alternate gene names: 82750200

Gene position: 489342-490253 (Clockwise)

Preceding gene: 82750199

Following gene: 82750201

Centisome position: 17.84

GC content: 32.46

Gene sequence:

>912_bases
ATGAATGAGGAAGTGAAAATGTTGGATAATAAAGATATTGCAACACCATCAAGAATGCGAGCGTTGTTAGATAAATATGG
CTTTAATTTTAAAAAAAGTTTAGGACAGAACTTTTTGATAGATGTGAATATCATTAATAATATCATTGATGCAAGTGATA
TTGATGCACAAACTGGGGTGATTGAAATTGGTCCAGGTATGGGGTCATTGACAGAACAATTGGCCAGACATGCTAAAAGA
GTATTGGCATTTGAAATTGATCAACGTTTAATACCTGTATTAAATGATACACTATCACCTTATGATAATGTGACGGTGAT
TAATGAAGATATTTTAAAAGCGAATATTAAAGAAGCTGTTGAAAATCATTTACAAGATTGTGAAAAAATAATGGTTGTTG
CAAACCTGCCGTACTATATTACGACGCCAATTTTATTAAATTTAATGCAACAAGATATACCAATTGATGGCTACGTGGTG
ATGATGCAAAAAGAAGTGGGCGAACGCTTAAATGCTGAAGTAGGTTCAAAAGCATATGGTTCATTATCAATTGTCGTACA
ATACTATACAGAGACTAGTAAAGTATTAACGGTACCTAAATCTGTATTTATGCCACCACCTAATGTTGATTCAATAGTTG
TAAAACTGATGCAGAGAACTGAACCGTTAGTAACAGTAGATAACGAGGAAGCATTCTTTAAGTTAGCAAAAGCAGCATTT
GCACAAAGAAGAAAGACAATTAACAATAACTATCAAAATTATTTTAAAGATGGTAAACAACACAAAGAAGTGATTTTACA
ATGGTTAGAACAAGCGGGTATTGATCCAAGACGTCGCGGTGAAACGCTATCTATTCAAGATTTTGCTAAATTGTATGAAG
AAAAGAAAAAATTCCCTCAATTAGAAAATTAA

Upstream 100 bases:

>100_bases
AGTAGAAAATTGCGAATCGGCCATTCCAATGGTAAGCAGTTATTGAAAAAGTTAAATGCATTTGGTTATACCGAAGCGGA
TGTAAGGCAAGCTTTAGAAG

Downstream 100 bases:

>100_bases
ATGATTGACAAAGCAAAGCACTATTGTTAAAATTTAAATTTTGTTTGACGAAAACGCTGCAAATATGGTATTATGTAATT
TGTAGCGAGGTGGAGCAATA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNEEVKMLDNKDIATPSRMRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPGMGSLTEQLARHAKR
VLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVV
MMQKEVGERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAF
AQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN

Sequences:

>Translated_303_residues
MNEEVKMLDNKDIATPSRMRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPGMGSLTEQLARHAKR
VLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVV
MMQKEVGERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAF
AQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN
>Mature_303_residues
MNEEVKMLDNKDIATPSRMRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPGMGSLTEQLARHAKR
VLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVV
MMQKEVGERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAF
AQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=294, Percent_Identity=30.2721088435374, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI7657198, Length=290, Percent_Identity=28.9655172413793, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1786236, Length=261, Percent_Identity=34.8659003831418, Blast_Score=148, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25141369, Length=298, Percent_Identity=28.5234899328859, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25146882, Length=223, Percent_Identity=33.6322869955157, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324989, Length=229, Percent_Identity=29.6943231441048, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357273, Length=293, Percent_Identity=29.6928327645051, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21358017, Length=298, Percent_Identity=29.1946308724832, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 34499; Mature: 34499

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEEVKMLDNKDIATPSRMRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGV
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCE
IEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAV
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHH
ENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCCC
SLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAF
CCEEEEEEHHCCCCEEECCHHHCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHH
AQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQ
HHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCC
LEN
CCC
>Mature Secondary Structure
MNEEVKMLDNKDIATPSRMRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGV
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCE
IEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAV
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHH
ENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCCC
SLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAF
CCEEEEEEHHCCCCEEECCHHHCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHH
AQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQ
HHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCC
LEN
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA