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The map label for this gene is hsdM

Identifier: 82750144

GI number: 82750144

Start: 420356

End: 421912

Strand: Direct

Name: hsdM

Synonym: SAB0384

Alternate gene names: 82750144

Gene position: 420356-421912 (Clockwise)

Preceding gene: 82750143

Following gene: 82750145

Centisome position: 15.33

GC content: 37.89

Gene sequence:

>1557_bases
ATGTCTATTACTGAAAAACAACGTCAGCAACAAGCTGAATTACATAAAAAATTATGGTCGATTGCGAATGATTTAAGAGG
AAATATGGATGCGAGTGAATTCCGTAATTACATTTTAGGCTTGATTTTCTATCGCTTCTTATCTGAAAAAGCCGAACAAG
AATACGCAGATGCCTTGTCGGGTGAAGACATCACGTATCAAGAAGCATGGGCAGATGAAGAATATCGTGAAGATTTAAAA
GCAGAATTAATTGATCAAGTCGGTTACTTCATTGAGCCACAAGATTTATTCAGCGCGATGATTCGTGAAATTGAAACGCA
AGATTTTGATATCGAACATCTGGCGACAGCAATTCGTAAAGTTGAAACATCAACATTAGGTGAAGAAAGTGAAAATGACT
TTATCGGACTGTTCAGCGATATGGACCTAAGTTCAACGCGACTAGGTAACAATGTCAAAGAACGTACTGCGTTAATTTCC
AAAGTTATGGTTAACCTTGACGATTTACCATTTGTTCACAGTGATATGGAAATTGATATGTTAGGTGATGCATACGAATT
TCTTATCGGGCGCTTTGCGGCGACAGCGGGTAAAAAAGCAGGCGAGTTCTATACACCACAACAAGTATCTAAGATACTGG
CGAAGATTGTTACAGACAGTAAAGATAAATTACGTCACGTGTATGACCCAACATGTGGTTCAGGTTCATTACTGTTACGT
GTTGGTAAAGAAACACAAGTGTATCGTTATTTTGGTCAAGAACGTAACAATACTACATACAACTTAGCACGCATGAACAT
GTTATTACATGATGTGCGTTATGAGAACTTCGATATCCGTAATGATGACACGTTGGAAAATCCAGCCTTTTTAGGTCATA
CATTTGATGCGGTTATTGCGAACCCACCATACAGTGCGAAATGGACAGCAGATTCAAAATTTGAAAATGATGAACGATTT
AGTGGTTACGGCAAGCTTGCGCCAAAATCCAAAGCAGACTTTGCCTTTATTCAACACATGGTACATTACTTAGACGATGA
AGGTACCATGGCAGTCGTACTTCCACATGGTGTATTATTCCGTGGTGCTTCAGAAGGCGTGATTCGTCGTTATTTAATTG
AAGAAAAGAACTACCTAGAAGCCGTGATTGGCTTACCAGCGAATATTTTCTATGGGACAAGTATTCCAACATGTATTTTA
GTATTTAAAAAATGTCGCCAACAAGACGACAACGTACTATTTATCGATGCATCCAATGATTTTGAAAAAGGAAAAAACCA
AAACCATTTAAGCGATGCCCAAGTCGAACGCATTATTAACACATATAAGCGTAAAGAAACAATTGATAAATATAGCTACA
GCGCGACACTACAAGAGATTGCCGATAACGATTACAACTTAAACATACCGCGATATGTTGATACATTCGAAGAAGAAGCA
CCGATTGATTTAGATCAAGTCCAACAAGATTTGAAAAATATCGACAAAGAAATCGCAGAAATCGAACAAGAAATCAATGC
ATACCTGAAAGAACTTGGGGTGTTGAAAGATGAGTAA

Upstream 100 bases:

>100_bases
CCTCCAACATCAAAATTCTCCCCATCGCAACATAGCCAAATGTTATAATAAATCTATTACACAAAGAGATAAATTACTTA
TTCAAAGGCGGAGGAATCAC

Downstream 100 bases:

>100_bases
TACACAAAAGAAAAATGTGCCAGAGTTGAGATTCCCAGGGTTTGAAGGCGAATGGGAAGAGAAGAAGTTAGGGGAAATTT
TTCAAATAATTTCTGGTTCA

Product: type I site-specific deoxyribonuclease

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLK
AELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDSKDKLRHVYDPTCGSGSLLLR
VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGHTFDAVIANPPYSAKWTADSKFENDERF
SGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGASEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCIL
VFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIINTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEA
PIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE

Sequences:

>Translated_518_residues
MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLK
AELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDSKDKLRHVYDPTCGSGSLLLR
VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGHTFDAVIANPPYSAKWTADSKFENDERF
SGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGASEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCIL
VFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIINTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEA
PIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE
>Mature_517_residues
SITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKA
ELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISK
VMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDSKDKLRHVYDPTCGSGSLLLRV
GKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGHTFDAVIANPPYSAKWTADSKFENDERFS
GYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLFRGASEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILV
FKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIINTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAP
IDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=501, Percent_Identity=24.750499001996, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 59512; Mature: 59381

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRK
CCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDM
HHHHHCCCCCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
LGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDSKDKLRHVYDPTCGSGSLLLR
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEE
VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGHTFDAVIA
ECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCHHHHCC
NPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLF
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCEEE
RGASEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASND
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCC
FEKGKNQNHLSDAQVERIINTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCC
PIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIRK
CCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEIDM
HHHHHCCCCCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
LGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDSKDKLRHVYDPTCGSGSLLLR
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEE
VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGHTFDAVIA
ECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCHHHHCC
NPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVLF
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCEEE
RGASEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASND
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCC
FEKGKNQNHLSDAQVERIINTYKRKETIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCC
PIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]