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The map label for this gene is engD [H]

Identifier: 82750075

GI number: 82750075

Start: 364479

End: 365576

Strand: Direct

Name: engD [H]

Synonym: SAB0314

Alternate gene names: 82750075

Gene position: 364479-365576 (Clockwise)

Preceding gene: 82750074

Following gene: 82750077

Centisome position: 13.29

GC content: 35.7

Gene sequence:

>1098_bases
ATGGCTTTAACAGCAGGTATCGTTGGCTTACCAAACGTTGGTAAATCAACATTATTTAATGCAATTACAAAGGCGGGTGC
CTTGGCAGCAAACTATCCGTTCGCAACTATAGACCCAAACGTGGGTATTGTAGAAGTGCCAGATGCAAGACTACTTAAAT
TAGAAGAAATGGTTCAACCTAAAAAAACATTACCTACTACATTTGAATTTACAGATATTGCCGGAATTGTTAAAGGTGCT
TCTAAAGGTGAAGGTTTAGGTAATAAATTCTTATCACATATTAGAGAAGTAGATGCAATTTGTCAGGTTGTTCGTGCTTT
TGCCGATGATAATGTAACACATGTAGCTGGTCGAGTTGATCCAATTGATGACATCGAAGTTATCAATATGGAATTAGTAC
TTGCAGACTTAGAATCAGTTGAAAAACGCTTACCTAGAATTGAAAAATTGGCACGTCAAAAAGATAAGACTGCTGAAATG
GAAGTGCGTATTTTAACAACTATTAAAGAAGCTTTAGAAAATGGTAAACCCGCTCGTAGTATTGACTTTAATGAAGAAGA
TCAAAAATGGGTGAATCAAGCGCAATTACTGACTTCTAAAAAAATGCTTTATATCGCTAATGTTGGTGAAGATGAAATTG
GTGATGATGATAATGATAAAGTAAAAGCGATTCGTGAATATGCAGCGCAAGAAGACTCTGAAGTGATTGTTATTAGTGCA
AAAATTGAAGAAGAAATTGCTACATTAGATGATGAAGATAAAGAAATGTTCTTAGAAGATTTAGGTATCGAAGAACCAGG
ATTAGATCGATTAATTAGAGCGACTTATGAATTATTAGGACTATCAACATATTTTACTGCTGGTGTGCAAGAAGTACATG
CTTGGACATTTAAACAAGGTATGACTGCACCTCAATGTGCTGGTATCATTCATACTGATTTTGAACGTGGATTTATCCGT
GCCGAAGTAACAAGTTATGACGACTATGTACAATATGGTGGCGAAAGTGGCGCTAAAGAAGCGGGCAGACAACGATTAGA
AGGTAAAGAATATATTATGCAAGATGGCGACATCGTTCATTTCAGATTTAATGTATAA

Upstream 100 bases:

>100_bases
ATTGTCAACGAAGCATTATGATTCCACGTCAAACGTTTGATAAAAAACTAAAAAAAATCATCGTATCTCAGGATGATACA
CAAAGATAGGAGAATGATTC

Downstream 100 bases:

>100_bases
ACGATAGAGTGAAGTTAATTAAATAGAATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCTTATA
ATTTATTAAAAGAATGGAAT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIVEVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGA
SKGEGLGNKFLSHIREVDAICQVVRAFADDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDNDKVKAIREYAAQEDSEVIVISA
KIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRATYELLGLSTYFTAGVQEVHAWTFKQGMTAPQCAGIIHTDFERGFIR
AEVTSYDDYVQYGGESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV

Sequences:

>Translated_365_residues
MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIVEVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGA
SKGEGLGNKFLSHIREVDAICQVVRAFADDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDNDKVKAIREYAAQEDSEVIVISA
KIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRATYELLGLSTYFTAGVQEVHAWTFKQGMTAPQCAGIIHTDFERGFIR
AEVTSYDDYVQYGGESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV
>Mature_364_residues
ALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIVEVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGAS
KGEGLGNKFLSHIREVDAICQVVRAFADDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEME
VRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDNDKVKAIREYAAQEDSEVIVISAK
IEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRATYELLGLSTYFTAGVQEVHAWTFKQGMTAPQCAGIIHTDFERGFIRA
EVTSYDDYVQYGGESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=368, Percent_Identity=43.75, Blast_Score=299, Evalue=3e-81,
Organism=Homo sapiens, GI58761502, Length=224, Percent_Identity=37.9464285714286, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI24308117, Length=100, Percent_Identity=40, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787454, Length=365, Percent_Identity=56.4383561643836, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1789574, Length=140, Percent_Identity=30, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17509631, Length=365, Percent_Identity=42.1917808219178, Blast_Score=299, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17508313, Length=101, Percent_Identity=34.6534653465347, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319499, Length=371, Percent_Identity=41.2398921832884, Blast_Score=258, Evalue=7e-70,
Organism=Saccharomyces cerevisiae, GI6321773, Length=380, Percent_Identity=38.4210526315789, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6321649, Length=155, Percent_Identity=37.4193548387097, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24640873, Length=366, Percent_Identity=41.8032786885246, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24640877, Length=366, Percent_Identity=41.8032786885246, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24640875, Length=366, Percent_Identity=41.8032786885246, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI24640879, Length=325, Percent_Identity=39.6923076923077, Blast_Score=231, Evalue=5e-61,
Organism=Drosophila melanogaster, GI24585318, Length=137, Percent_Identity=32.8467153284672, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40502; Mature: 40371

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIVEVPDARLLKLEEMVQP
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCEEECCCHHHHHHHHHHCC
KKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQVVRAFADDNVTHVAGRVD
HHCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
PIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEMEVRILTTIKEALENGKPARS
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
IDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDNDKVKAIREYAAQEDSEVIVISA
CCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
KIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRATYELLGLSTYFTAGVQEVHAWTFKQG
CHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGGESGAKEAGRQRLEGKEYIMQDGDIVH
CCCCHHCCCEECCCCCCEEEEEECCHHHHHHHCCCCCHHHHHHHHCCCHHHHHCCCCEEE
FRFNV
EEECC
>Mature Secondary Structure 
ALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIVEVPDARLLKLEEMVQP
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCEEECCCHHHHHHHHHHCC
KKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQVVRAFADDNVTHVAGRVD
HHCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
PIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEMEVRILTTIKEALENGKPARS
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
IDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDNDKVKAIREYAAQEDSEVIVISA
CCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
KIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRATYELLGLSTYFTAGVQEVHAWTFKQG
CHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGGESGAKEAGRQRLEGKEYIMQDGDIVH
CCCCHHCCCEECCCCCCEEEEEECCHHHHHHHCCCCCHHHHHHHHCCCHHHHHCCCCEEE
FRFNV
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]