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The map label for this gene is 82750028

Identifier: 82750028

GI number: 82750028

Start: 317562

End: 318794

Strand: Direct

Name: 82750028

Synonym: SAB0265

Alternate gene names: NA

Gene position: 317562-318794 (Clockwise)

Preceding gene: 82750027

Following gene: 82750029

Centisome position: 11.58

GC content: 31.95

Gene sequence:

>1233_bases
ATGAGTAATACCCAAAAGAAAAATGTGCCAGAGTTGAGATTCCCGGGGTTTGAAGGCGAATGGGAAGAGAAGAAGTTAGG
GGATGTAGCAACTTTTGCCAAAGGTAAGTTAGGCGCTAAGAAAGATGTGAGTCAAAATGGTGTGCCAATAATATTGTATG
GAGAATTATATACAAAGTATGGTGCTATTGTTAGCAAGATTTTTAGTAAAACAGATATTCCCGAAAACAAATTAAAGATA
GCTAAAAAAAATGACGTTCTTATTCCATCATCTGGGGAAACAGCAATTGATATAGCAACTGCATCGTGCATTTATTTAAA
TAAAGGCGTTGCAGTTGGTGGAGACATTAATATTTTAACACCTCAAAAGCAAGACGATAGGTTTATTTCATTGTCAATTA
ATGGAATAAATAAAAATGAATTATCTAAATATGCACAAGGGAAGACAGTAGTTCATTTATATAATAATGATATTAAAAAT
TTGAAAATAGTATTTCCAAGCGAATTTGAAGAACAAGTAAGAATAGGTGACTTCTTCAGCAAACTCGATCGACAAATTGA
ATTAGAAGAACAAAAACTTGAATTACTTCAACAACAGAAAAAAGGCTATATGCAGAAAATCTTCTCACAGGAATTGCGAT
TCAAAGATGAGAATGGTGAAGAATATCCAGAGTGGGAAGAGAAGCAATTAGGGGAAGTAGCCGAAATAATAGGGGGAGGA
CCTCCAAGTACAAAAAACAAATTATATTGGAATGGTGAAATAAATTGGTTTTCACCGATTGAAATAGGTAACAAAACATA
TGTCTATAGTAGTCAAAAGAAAATAACAGAGGAAGGGCTACGAAAAAGTTCTGCTAAAATTTTGCCTGTGGGCACTATAC
TATTTACCTCAAGAGCTGGAATAGGAAAAACAGCTATCTTAGCTAAAGAATCTACAACGAATCAGGGATTTCAATCAATA
GTACCTCGAAAAGGTGTGTTAGATTCGTATTATGTATATACTATTTCAAATATATTGAAAATATTAGCAGAAAAAGTTAG
TGCAGGGTCAACATTTTCAGAAATATCAAAAAAGCAAATGGAGCAATTGAATTTAAATATACCGATGATAAAGGAACAAA
AAAATATAAGTAAATTCTTTAGCAAATTTGATAACTTAATAGAAATACAGGAACGAAAACTCAAACTATTAAAACAACAG
AAACAAGGTTTACTACAAAAAATGTTTATATAG

Upstream 100 bases:

>100_bases
ATCAAACATGTTAATATATTTTGGTAAGTAAACGTGACTACACATCTTAGTTTTAATTTGATGTTGCGTCACATGATACT
AATAAGAGGTGTGTGGCGTA

Downstream 100 bases:

>100_bases
AACAGACGGCTTATTGGTCGTCTTTTTATTTTGGATAAAAGGAGCAAACAAATGGAAACAAAAGTAATAACAAGATATAT
TGTATTAATTTTAGCATTAG

Product: type-I specificity determinant subunit

Products: NA

Alternate protein names: S.MjaXIP; Type I restriction enzyme MjaXIP specificity protein; S protein [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSNTQKKNVPELRFPGFEGEWEEKKLGDVATFAKGKLGAKKDVSQNGVPIILYGELYTKYGAIVSKIFSKTDIPENKLKI
AKKNDVLIPSSGETAIDIATASCIYLNKGVAVGGDINILTPQKQDDRFISLSINGINKNELSKYAQGKTVVHLYNNDIKN
LKIVFPSEFEEQVRIGDFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEEYPEWEEKQLGEVAEIIGGG
PPSTKNKLYWNGEINWFSPIEIGNKTYVYSSQKKITEEGLRKSSAKILPVGTILFTSRAGIGKTAILAKESTTNQGFQSI
VPRKGVLDSYYVYTISNILKILAEKVSAGSTFSEISKKQMEQLNLNIPMIKEQKNISKFFSKFDNLIEIQERKLKLLKQQ
KQGLLQKMFI

Sequences:

>Translated_410_residues
MSNTQKKNVPELRFPGFEGEWEEKKLGDVATFAKGKLGAKKDVSQNGVPIILYGELYTKYGAIVSKIFSKTDIPENKLKI
AKKNDVLIPSSGETAIDIATASCIYLNKGVAVGGDINILTPQKQDDRFISLSINGINKNELSKYAQGKTVVHLYNNDIKN
LKIVFPSEFEEQVRIGDFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEEYPEWEEKQLGEVAEIIGGG
PPSTKNKLYWNGEINWFSPIEIGNKTYVYSSQKKITEEGLRKSSAKILPVGTILFTSRAGIGKTAILAKESTTNQGFQSI
VPRKGVLDSYYVYTISNILKILAEKVSAGSTFSEISKKQMEQLNLNIPMIKEQKNISKFFSKFDNLIEIQERKLKLLKQQ
KQGLLQKMFI
>Mature_409_residues
SNTQKKNVPELRFPGFEGEWEEKKLGDVATFAKGKLGAKKDVSQNGVPIILYGELYTKYGAIVSKIFSKTDIPENKLKIA
KKNDVLIPSSGETAIDIATASCIYLNKGVAVGGDINILTPQKQDDRFISLSINGINKNELSKYAQGKTVVHLYNNDIKNL
KIVFPSEFEEQVRIGDFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEEYPEWEEKQLGEVAEIIGGGP
PSTKNKLYWNGEINWFSPIEIGNKTYVYSSQKKITEEGLRKSSAKILPVGTILFTSRAGIGKTAILAKESTTNQGFQSIV
PRKGVLDSYYVYTISNILKILAEKVSAGSTFSEISKKQMEQLNLNIPMIKEQKNISKFFSKFDNLIEIQERKLKLLKQQK
QGLLQKMFI

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 46378; Mature: 46247

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTQKKNVPELRFPGFEGEWEEKKLGDVATFAKGKLGAKKDVSQNGVPIILYGELYTKY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHH
GAIVSKIFSKTDIPENKLKIAKKNDVLIPSSGETAIDIATASCIYLNKGVAVGGDINILT
HHHHHHHHHHCCCCHHHEEEECCCCEEECCCCCCEEEEEEEEEEEEECCEEECCCEEEEC
PQKQDDRFISLSINGINKNELSKYAQGKTVVHLYNNDIKNLKIVFPSEFEEQVRIGDFFS
CCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHH
KLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEEYPEWEEKQLGEVAEIIGGG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCC
PPSTKNKLYWNGEINWFSPIEIGNKTYVYSSQKKITEEGLRKSSAKILPVGTILFTSRAG
CCCCCCEEEECCCCCEECCEECCCEEEEECCHHHHHHHHHHHCCCCEEEEEEEEEECCCC
IGKTAILAKESTTNQGFQSIVPRKGVLDSYYVYTISNILKILAEKVSAGSTFSEISKKQM
CCCEEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EQLNLNIPMIKEQKNISKFFSKFDNLIEIQERKLKLLKQQKQGLLQKMFI
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNTQKKNVPELRFPGFEGEWEEKKLGDVATFAKGKLGAKKDVSQNGVPIILYGELYTKY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHH
GAIVSKIFSKTDIPENKLKIAKKNDVLIPSSGETAIDIATASCIYLNKGVAVGGDINILT
HHHHHHHHHHCCCCHHHEEEECCCCEEECCCCCCEEEEEEEEEEEEECCEEECCCEEEEC
PQKQDDRFISLSINGINKNELSKYAQGKTVVHLYNNDIKNLKIVFPSEFEEQVRIGDFFS
CCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHH
KLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEEYPEWEEKQLGEVAEIIGGG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCC
PPSTKNKLYWNGEINWFSPIEIGNKTYVYSSQKKITEEGLRKSSAKILPVGTILFTSRAG
CCCCCCEEEECCCCCEECCEECCCEEEEECCHHHHHHHHHHHCCCCEEEEEEEEEECCCC
IGKTAILAKESTTNQGFQSIVPRKGVLDSYYVYTISNILKILAEKVSAGSTFSEISKKQM
CCCEEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EQLNLNIPMIKEQKNISKFFSKFDNLIEIQERKLKLLKQQKQGLLQKMFI
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]