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The map label for this gene is gutB [H]

Identifier: 82749954

GI number: 82749954

Start: 234482

End: 235537

Strand: Direct

Name: gutB [H]

Synonym: SAB0187

Alternate gene names: 82749954

Gene position: 234482-235537 (Clockwise)

Preceding gene: 82749953

Following gene: 82749955

Centisome position: 8.55

GC content: 36.65

Gene sequence:

>1056_bases
GTGAAAGCTTTAGTAAAAACAAGAGAAGGACATGGCAACTTAGAACTTCTTGATAAAGAAGTTGCAACACCGCTAGATGA
TAAAGTGAAGATTAAAGTACATTATGCAGGAATTTGTGGCACAGATATTCATACTTATGAAGGTCATTATAAAGTTAATT
TTCCAGTGACATTAGGTCATGAATTTTCTGGTGAAATCGTTGAAGTTGGAGCAGACGTTAAAGATTTTAAAGTTGGTGAC
CGTGTCACTTCTGAAACGACATTCTATGTTTGTAATGAGTGTGAATACTGTGAATCAAAAGACTATAATTTATGCAACCA
TCGAAAAGGTATTGGAACACAAGTTGATGGCGCATTTACTAATTATGTCATTGCACGTGAAGAAAGTTTGCATCATATTC
CAGATGAAGTATCGTATCAGTCTGCAGCTATGACAGAACCATTAGCATGTGCACATCATGGCGTTTCTAAGATTCAAGTG
AACTCTGGCGATGTAGCAGTTGTAATGGGACCTGGGCCAATCGGATTACTTGTAGCACAAATGTTGAAAAGTAAAGGCGC
AACTGTTGTGGTAACTGGATTGGACAATGACAAAGTCAGATTAGATAAAGCAGAAGCATTGCACATGGATTATGTAGTCA
ATTTACAACAAACAGACTTAAAAACGTATATCAATGGAATTACAGACGGTTACGGTGCAGATGTTGTTGTTGAATGTTCA
GGTGCAGTTCCAGCAGCACGACAAGGTTTGGATATTTTACGCAAAAAAGGTTTCTACAGTCAAATAGGTATTTTTAAGGA
TGCTGAAATTCCATTTGATATGGAAAAAGTGATTCAAAAAGAAATAACAGTTGTTGGTAGTAGAAGTCAAAAGCCAGCAG
ATTGGGAACCTTCATTGCAACTTATGGCGGATGGTTTAGTAAATGCTGAAGCTTTGGTGACAAAAATATATGATATTTCG
AAATGGGACGAGTCGTATCAACATTTAAAATCTGGCGAAGGTATTAAAGCATTACTTAAGCCGCTCGATTTAGATGAAAA
TGAAGGAGAGAATTAA

Upstream 100 bases:

>100_bases
GTTCATCTGAAATTTCAAGTATTGATCAAGGTGGTAATATCGTTAACTATCTTATCTTTAAACTATTTAGTCTATTCAAT
TAAAAATCGAGGTGTTTGTG

Downstream 100 bases:

>100_bases
TATGGTAGAATCAATGCTAACTTTTATGCTTGGGCCATTGAGACAAATCACTGATTTTTATATGGAACATTTACTCGTAA
GTAATTCCATCGTCATTGTA

Product: zinc-binding sorbitol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIHTYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGD
RVTSETTFYVCNECEYCESKDYNLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
NSGDVAVVMGPGPIGLLVAQMLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQTDLKTYINGITDGYGADVVVECS
GAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEKVIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDIS
KWDESYQHLKSGEGIKALLKPLDLDENEGEN

Sequences:

>Translated_351_residues
MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIHTYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGD
RVTSETTFYVCNECEYCESKDYNLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
NSGDVAVVMGPGPIGLLVAQMLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQTDLKTYINGITDGYGADVVVECS
GAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEKVIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDIS
KWDESYQHLKSGEGIKALLKPLDLDENEGEN
>Mature_351_residues
MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIHTYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGD
RVTSETTFYVCNECEYCESKDYNLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
NSGDVAVVMGPGPIGLLVAQMLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQTDLKTYINGITDGYGADVVVECS
GAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEKVIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDIS
KWDESYQHLKSGEGIKALLKPLDLDENEGEN

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=346, Percent_Identity=28.9017341040462, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI4501933, Length=360, Percent_Identity=26.1111111111111, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI4501929, Length=356, Percent_Identity=26.4044943820225, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI71743840, Length=213, Percent_Identity=31.4553990610329, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI262073058, Length=213, Percent_Identity=31.4553990610329, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI156523966, Length=359, Percent_Identity=26.1838440111421, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI34577061, Length=361, Percent_Identity=26.5927977839335, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI71565154, Length=271, Percent_Identity=29.1512915129151, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4501939, Length=353, Percent_Identity=26.0623229461756, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1788075, Length=365, Percent_Identity=30.4109589041096, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1790045, Length=347, Percent_Identity=31.1239193083574, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788073, Length=325, Percent_Identity=30.4615384615385, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI226510992, Length=312, Percent_Identity=31.0897435897436, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1787863, Length=336, Percent_Identity=30.6547619047619, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790718, Length=306, Percent_Identity=27.1241830065359, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788407, Length=297, Percent_Identity=26.2626262626263, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082125, Length=340, Percent_Identity=26.7647058823529, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786552, Length=366, Percent_Identity=28.6885245901639, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI87081918, Length=322, Percent_Identity=26.3975155279503, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786825, Length=375, Percent_Identity=24.2666666666667, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787572, Length=283, Percent_Identity=28.2685512367491, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786518, Length=221, Percent_Identity=28.5067873303167, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=343, Percent_Identity=30.3206997084548, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17562878, Length=341, Percent_Identity=29.3255131964809, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71988145, Length=339, Percent_Identity=28.6135693215339, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17562584, Length=338, Percent_Identity=28.698224852071, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17562582, Length=336, Percent_Identity=26.1904761904762, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI25146526, Length=361, Percent_Identity=26.0387811634349, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71997431, Length=363, Percent_Identity=25.8953168044077, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17565904, Length=348, Percent_Identity=25.5747126436782, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322619, Length=344, Percent_Identity=31.6860465116279, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323099, Length=310, Percent_Identity=31.9354838709677, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319955, Length=344, Percent_Identity=31.1046511627907, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319258, Length=388, Percent_Identity=28.6082474226804, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324486, Length=333, Percent_Identity=28.2282282282282, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319257, Length=342, Percent_Identity=27.4853801169591, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319621, Length=333, Percent_Identity=27.3273273273273, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323961, Length=333, Percent_Identity=27.3273273273273, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6323729, Length=333, Percent_Identity=25.5255255255255, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320033, Length=367, Percent_Identity=25.3405994550409, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323980, Length=240, Percent_Identity=26.25, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17137530, Length=350, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17737897, Length=355, Percent_Identity=28.7323943661972, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17737895, Length=366, Percent_Identity=27.3224043715847, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI221457811, Length=313, Percent_Identity=24.2811501597444, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45550770, Length=313, Percent_Identity=24.2811501597444, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45551930, Length=313, Percent_Identity=24.2811501597444, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 38506; Mature: 38506

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIHTYEGHYKVNFPVTLGH
CCCCEECCCCCCCEEEECHHHCCCCCCCEEEEEEEEEEECCCCEEECCEEEEEEEEEECC
EFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCESKDYNLCNHRKGIGTQVDGAFT
CCCCCEEEECCCCHHEECCCCCCCCEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCHH
NYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQVNSGDVAVVMGPGPIGLLVAQ
EEEEEECCCHHCCCCHHCCCHHHCCCCHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHH
MLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQTDLKTYINGITDGYGADVVVECS
HHHCCCCEEEEECCCCCCEEECHHHEEEEEEEEECHHHHHHHHHHHCCCCCCCCEEEEEC
GAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEKVIQKEITVVGSRSQKPADWEPSLQ
CCCCHHHHHHHHHHHCCCHHHHCEEECCCCCCCHHHHHHHHHEEECCCCCCCCCCCCCHH
LMADGLVNAEALVTKIYDISKWDESYQHLKSGEGIKALLKPLDLDENEGEN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIHTYEGHYKVNFPVTLGH
CCCCEECCCCCCCEEEECHHHCCCCCCCEEEEEEEEEEECCCCEEECCEEEEEEEEEECC
EFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCESKDYNLCNHRKGIGTQVDGAFT
CCCCCEEEECCCCHHEECCCCCCCCEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCHH
NYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQVNSGDVAVVMGPGPIGLLVAQ
EEEEEECCCHHCCCCHHCCCHHHCCCCHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHH
MLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQTDLKTYINGITDGYGADVVVECS
HHHCCCCEEEEECCCCCCEEECHHHEEEEEEEEECHHHHHHHHHHHCCCCCCCCEEEEEC
GAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEKVIQKEITVVGSRSQKPADWEPSLQ
CCCCHHHHHHHHHHHCCCHHHHCEEECCCCCCCHHHHHHHHHEEECCCCCCCCCCCCCHH
LMADGLVNAEALVTKIYDISKWDESYQHLKSGEGIKALLKPLDLDENEGEN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]