Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 82703888

GI number: 82703888

Start: 3180223

End: 3181581

Strand: Reverse

Name: trmE

Synonym: Nmul_A2775

Alternate gene names: 82703888

Gene position: 3181581-3180223 (Counterclockwise)

Preceding gene: 82703889

Following gene: 82703886

Centisome position: 99.92

GC content: 55.78

Gene sequence:

>1359_bases
ATGACCCGTTCTGATGTGATTGCCGCGATTGCAACGCCTCCGGGTCGGGGAGGTATCGGGGTGGTGCGTGTTTCCGGCAG
AAACCTGCAATCGCTTGCTCTCAAGGTCGCAGGCTGCGTGCCGGAACCCCGGCGCGCCAGCCTCACCCGATTTCGCGACG
AAAATGATCGCGTTATCGATCAGGGCATAGCCCTCTACTTTCCCGCTCCCCAGTCCTATACCGGAGAAGAGGTTCTGGAA
CTGCAAGGACACGGCGGCCCTGCAGTCATGAACCTGTTGCTGGCAAGTTGCCTTTCCGCTGGTGCGCGACTGGCGCAACC
CGGGGAGTTTACCTTGCGTGCATTCCTGAATAACAAGCTTGATCTGGCGCAGGCTGAAAGCGTGGCAGACCTTATTGACG
CCAGTACCGAGGAAGCCGCCCGCTGCGCCATCCGTTCCCTTCAAGGCGAATTTTCCAACGCTATTCATACTTCAGTGCAA
GCACTTACTGACCTGCGAATGCTTGTCGAGGCTTCACTTGATTTTTCAGAAGAAGAAATTGAGTTCATTTCTGGAAGGGA
ACTGGAATTCAGATTGGAGCACATCCGGCAACAGCTTGAACAGGTTTTCAGCGCGGCGCGGCAAGGTAGCCTGCTGCGAG
AAGGAATATGGATTGCTTTGGTCGGACAGCCGAATGTGGGAAAATCGAGCCTCCTGAATCGACTCGCCGGAGAAGAAGTG
GCTCTCGTTACGGAGGTCCCGGGCACGACACGGGACGTTATCCGGCAGGTCATAGAGATTGAAGGTGTGCCCATGCATCT
GCTGGATACCGCCGGCTTGAGGGAGACGGAAGATGCGATCGAAAAAATGGGTATGGCGCGGACACGCTCCACGATCGATA
AAGCAAGTATCGTCCTGCTGCTTGTGGATAGCCGAGTGGGAATAACGCCAGAAGATCAGGCCATACTCGCGTCGCTTCCC
CCGGGTTTGCGGCTCATTGTCGTCCATAACAAAACCGACCTGCTCGAATCTCCCCCGAATTCCACTGTCTCCACCGCTAC
AGCTACTGCAGATATCTGGGTTTCCGCTAAAACGGGGGCGGGCATCGGATCCCTCCAGCAAGGATTGCTGGAAATGATAG
GATGGCAGCCATCAACAGGAGAAGGAGCCTTCATGGCGCGCCAGCGTCATCTTTCTGCATTGACAGCTGCCCGAACCCAT
CTTGAGGCTGCTTGTGCGCTGGCAAACAGCCTGGATCAGGTCGAGCTTTTTGCTGAGGAACTGCGCCTTGCCCAACTGGC
TTTATCTTCGATTACCGGTGAATTCAGCGCCGACGACCTGCTGGGCGAAATTTTTTCCCGCTTCTGCATAGGAAAGTAG

Upstream 100 bases:

>100_bases
CGAGCAAGCAGAAGAAAGCAAATTAGTGATCAGGGGAATTGCCGGACCCGACTCCCCTCTCTTCCCGTCTTCATAAGCGA
GGTTGGCATTCGAACTCAGG

Downstream 100 bases:

>100_bases
CAGAACCTCTCGTTTCCGGGCAGATCCTTCCGCTCAAGTTTCAGGCTGGCTCTCGCTTGACTTGTTCTCGCCCCGGTTAT
CCCGGTTATTGCCGCTGGAC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTRSDVIAAIATPPGRGGIGVVRVSGRNLQSLALKVAGCVPEPRRASLTRFRDENDRVIDQGIALYFPAPQSYTGEEVLE
LQGHGGPAVMNLLLASCLSAGARLAQPGEFTLRAFLNNKLDLAQAESVADLIDASTEEAARCAIRSLQGEFSNAIHTSVQ
ALTDLRMLVEASLDFSEEEIEFISGRELEFRLEHIRQQLEQVFSAARQGSLLREGIWIALVGQPNVGKSSLLNRLAGEEV
ALVTEVPGTTRDVIRQVIEIEGVPMHLLDTAGLRETEDAIEKMGMARTRSTIDKASIVLLLVDSRVGITPEDQAILASLP
PGLRLIVVHNKTDLLESPPNSTVSTATATADIWVSAKTGAGIGSLQQGLLEMIGWQPSTGEGAFMARQRHLSALTAARTH
LEAACALANSLDQVELFAEELRLAQLALSSITGEFSADDLLGEIFSRFCIGK

Sequences:

>Translated_452_residues
MTRSDVIAAIATPPGRGGIGVVRVSGRNLQSLALKVAGCVPEPRRASLTRFRDENDRVIDQGIALYFPAPQSYTGEEVLE
LQGHGGPAVMNLLLASCLSAGARLAQPGEFTLRAFLNNKLDLAQAESVADLIDASTEEAARCAIRSLQGEFSNAIHTSVQ
ALTDLRMLVEASLDFSEEEIEFISGRELEFRLEHIRQQLEQVFSAARQGSLLREGIWIALVGQPNVGKSSLLNRLAGEEV
ALVTEVPGTTRDVIRQVIEIEGVPMHLLDTAGLRETEDAIEKMGMARTRSTIDKASIVLLLVDSRVGITPEDQAILASLP
PGLRLIVVHNKTDLLESPPNSTVSTATATADIWVSAKTGAGIGSLQQGLLEMIGWQPSTGEGAFMARQRHLSALTAARTH
LEAACALANSLDQVELFAEELRLAQLALSSITGEFSADDLLGEIFSRFCIGK
>Mature_451_residues
TRSDVIAAIATPPGRGGIGVVRVSGRNLQSLALKVAGCVPEPRRASLTRFRDENDRVIDQGIALYFPAPQSYTGEEVLEL
QGHGGPAVMNLLLASCLSAGARLAQPGEFTLRAFLNNKLDLAQAESVADLIDASTEEAARCAIRSLQGEFSNAIHTSVQA
LTDLRMLVEASLDFSEEEIEFISGRELEFRLEHIRQQLEQVFSAARQGSLLREGIWIALVGQPNVGKSSLLNRLAGEEVA
LVTEVPGTTRDVIRQVIEIEGVPMHLLDTAGLRETEDAIEKMGMARTRSTIDKASIVLLLVDSRVGITPEDQAILASLPP
GLRLIVVHNKTDLLESPPNSTVSTATATADIWVSAKTGAGIGSLQQGLLEMIGWQPSTGEGAFMARQRHLSALTAARTHL
EAACALANSLDQVELFAEELRLAQLALSSITGEFSADDLLGEIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=466, Percent_Identity=34.7639484978541, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI193082993, Length=456, Percent_Identity=35.0877192982456, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI193082988, Length=498, Percent_Identity=32.7309236947791, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI2367268, Length=460, Percent_Identity=50.8695652173913, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI87082120, Length=168, Percent_Identity=38.0952380952381, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17507259, Length=445, Percent_Identity=31.9101123595506, Blast_Score=178, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=495, Percent_Identity=32.3232323232323, Blast_Score=210, Evalue=4e-55,
Organism=Drosophila melanogaster, GI45550813, Length=471, Percent_Identity=33.3333333333333, Blast_Score=205, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_NITMU (Q2Y5A9)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413454.1
- HSSP:   Q9WYA4
- ProteinModelPortal:   Q2Y5A9
- SMR:   Q2Y5A9
- STRING:   Q2Y5A9
- GeneID:   3785625
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2775
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q2Y5A9
- ProtClustDB:   PRK05291
- BioCyc:   NMUL323848:NMUL_A2775-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48535; Mature: 48404

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 80-80 BINDING 119-119 BINDING 452-452

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSDVIAAIATPPGRGGIGVVRVSGRNLQSLALKVAGCVPEPRRASLTRFRDENDRVID
CCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHH
QGIALYFPAPQSYTGEEVLELQGHGGPAVMNLLLASCLSAGARLAQPGEFTLRAFLNNKL
CCEEEEECCCCCCCCCHHHEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
DLAQAESVADLIDASTEEAARCAIRSLQGEFSNAIHTSVQALTDLRMLVEASLDFSEEEI
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
EFISGRELEFRLEHIRQQLEQVFSAARQGSLLREGIWIALVGQPNVGKSSLLNRLAGEEV
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEECCCCCCHHHHHHHHCCCCE
ALVTEVPGTTRDVIRQVIEIEGVPMHLLDTAGLRETEDAIEKMGMARTRSTIDKASIVLL
EEEEECCCCHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHCHHHHHHHHHCCEEEEE
LVDSRVGITPEDQAILASLPPGLRLIVVHNKTDLLESPPNSTVSTATATADIWVSAKTGA
EECCCCCCCCCCCCCEECCCCCEEEEEEECCHHHHCCCCCCCCCCCEEEEEEEEEECCCC
GIGSLQQGLLEMIGWQPSTGEGAFMARQRHLSALTAARTHLEAACALANSLDQVELFAEE
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLAQLALSSITGEFSADDLLGEIFSRFCIGK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRSDVIAAIATPPGRGGIGVVRVSGRNLQSLALKVAGCVPEPRRASLTRFRDENDRVID
CCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHH
QGIALYFPAPQSYTGEEVLELQGHGGPAVMNLLLASCLSAGARLAQPGEFTLRAFLNNKL
CCEEEEECCCCCCCCCHHHEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
DLAQAESVADLIDASTEEAARCAIRSLQGEFSNAIHTSVQALTDLRMLVEASLDFSEEEI
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
EFISGRELEFRLEHIRQQLEQVFSAARQGSLLREGIWIALVGQPNVGKSSLLNRLAGEEV
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEECCCCCCHHHHHHHHCCCCE
ALVTEVPGTTRDVIRQVIEIEGVPMHLLDTAGLRETEDAIEKMGMARTRSTIDKASIVLL
EEEEECCCCHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHCHHHHHHHHHCCEEEEE
LVDSRVGITPEDQAILASLPPGLRLIVVHNKTDLLESPPNSTVSTATATADIWVSAKTGA
EECCCCCCCCCCCCCEECCCCCEEEEEEECCHHHHCCCCCCCCCCCEEEEEEEEEECCCC
GIGSLQQGLLEMIGWQPSTGEGAFMARQRHLSALTAARTHLEAACALANSLDQVELFAEE
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLAQLALSSITGEFSADDLLGEIFSRFCIGK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA