Click here to switch to the map view.

The map label for this gene is gidB

Identifier: 82703882

GI number: 82703882

Start: 3174450

End: 3175100

Strand: Reverse

Name: gidB

Synonym: Nmul_A2769

Alternate gene names: 82703882

Gene position: 3175100-3174450 (Counterclockwise)

Preceding gene: 162139859

Following gene: 82703881

Centisome position: 99.71

GC content: 56.84

Gene sequence:

>651_bases
ATGAATCCGGCTACACAACTGGCTGACGGAATCGCAACACTGGGACTATCTATTACCGAATCAAATCAGGCACGCCTCCT
GCAATATCTGGCGCTCATCCAGAAATGGAACCGGGTGCACAATCTGACGGCAGTGCGCGAACCGGAAGCGATGCTCGCAT
TGCATGTGCTGGACAGTCTGGCTGTTCTGCCGCACATTGGCGGCTCCCGCATTGCGGATGTGGGCAGCGGCGCCGGTCTT
CCTGGAATCCCAGTGGCGTTGGCCCGCCCGGAGTGGCGTGTGGTGCTAGTGGAAAGCAATCATAAAAAAGCCGCGTTCCT
GCAGCAGGCGCGGATTGAATTGGGGCTTGAAAATGTGGAAGTCATCGGGGAGCGCATGGAAGGCGTCCGCTCGAACGCCG
GCTTCAATACCGTTATTTCGCGCGCTTTTTCCGATCTGGCGGATTTCGTCAAGCTCGCGGGACATTTGTGTGCACGGGGG
GAGGAGCAAGGGACTGACTGTGGGCGGTTGGTGGCAATGAAAGGCGTATATCCTCACGAAGAACTTGCGCAGTTACCCGA
GACATTCATTGTCGACAACATATTGTCGCTAACGATTCCCGGCCTGAGGGCGAAGCGTCATCTGGTTGTGCTCAAGCGTG
CCGGACAGTAA

Upstream 100 bases:

>100_bases
GACAGGCATCCCGTATCTCCGGAATCACGCCCGCCGCAATTTCGCTATTGCTGGTCCATGTCAAGCGCGGTTTTTCCAGC
GCGAATACGAAGAAAAGCGC

Downstream 100 bases:

>100_bases
AGTAAGATTCCTTGGCTTTTCGCCAAGTTTTTCACCTCTTATTGATCGGCTTCCGGTATCTCGGTTCTAGGAGTGGCAAT
GGCGAAAATTCTGGCAGTAA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MNPATQLADGIATLGLSITESNQARLLQYLALIQKWNRVHNLTAVREPEAMLALHVLDSLAVLPHIGGSRIADVGSGAGL
PGIPVALARPEWRVVLVESNHKKAAFLQQARIELGLENVEVIGERMEGVRSNAGFNTVISRAFSDLADFVKLAGHLCARG
EEQGTDCGRLVAMKGVYPHEELAQLPETFIVDNILSLTIPGLRAKRHLVVLKRAGQ

Sequences:

>Translated_216_residues
MNPATQLADGIATLGLSITESNQARLLQYLALIQKWNRVHNLTAVREPEAMLALHVLDSLAVLPHIGGSRIADVGSGAGL
PGIPVALARPEWRVVLVESNHKKAAFLQQARIELGLENVEVIGERMEGVRSNAGFNTVISRAFSDLADFVKLAGHLCARG
EEQGTDCGRLVAMKGVYPHEELAQLPETFIVDNILSLTIPGLRAKRHLVVLKRAGQ
>Mature_216_residues
MNPATQLADGIATLGLSITESNQARLLQYLALIQKWNRVHNLTAVREPEAMLALHVLDSLAVLPHIGGSRIADVGSGAGL
PGIPVALARPEWRVVLVESNHKKAAFLQQARIELGLENVEVIGERMEGVRSNAGFNTVISRAFSDLADFVKLAGHLCARG
EEQGTDCGRLVAMKGVYPHEELAQLPETFIVDNILSLTIPGLRAKRHLVVLKRAGQ

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=198, Percent_Identity=43.9393939393939, Blast_Score=176, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_NITMU (Q2Y5B5)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413448.1
- ProteinModelPortal:   Q2Y5B5
- SMR:   Q2Y5B5
- STRING:   Q2Y5B5
- GeneID:   3785364
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2769
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- PhylomeDB:   Q2Y5B5
- ProtClustDB:   PRK00107
- BioCyc:   NMUL323848:NMUL_A2769-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23357; Mature: 23357

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPATQLADGIATLGLSITESNQARLLQYLALIQKWNRVHNLTAVREPEAMLALHVLDSL
CCHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AVLPHIGGSRIADVGSGAGLPGIPVALARPEWRVVLVESNHKKAAFLQQARIELGLENVE
HHHCCCCCCHHHHCCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHCHHHHH
VIGERMEGVRSNAGFNTVISRAFSDLADFVKLAGHLCARGEEQGTDCGRLVAMKGVYPHE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCHH
ELAQLPETFIVDNILSLTIPGLRAKRHLVVLKRAGQ
HHHHCHHHHHHHHHHHHHCCCCCHHHHEEEEECCCC
>Mature Secondary Structure
MNPATQLADGIATLGLSITESNQARLLQYLALIQKWNRVHNLTAVREPEAMLALHVLDSL
CCHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AVLPHIGGSRIADVGSGAGLPGIPVALARPEWRVVLVESNHKKAAFLQQARIELGLENVE
HHHCCCCCCHHHHCCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHCHHHHH
VIGERMEGVRSNAGFNTVISRAFSDLADFVKLAGHLCARGEEQGTDCGRLVAMKGVYPHE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCHH
ELAQLPETFIVDNILSLTIPGLRAKRHLVVLKRAGQ
HHHHCHHHHHHHHHHHHHCCCCCHHHHEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA