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The map label for this gene is mlaE [H]

Identifier: 82703861

GI number: 82703861

Start: 3150955

End: 3151752

Strand: Reverse

Name: mlaE [H]

Synonym: Nmul_A2748

Alternate gene names: 82703861

Gene position: 3151752-3150955 (Counterclockwise)

Preceding gene: 82703862

Following gene: 82703860

Centisome position: 98.98

GC content: 56.39

Gene sequence:

>798_bases
TTGCCTAGGCGTATCGTAACTGGTATCCAGAGCGTCGGTCATCGCGTCATAGACAGTATCTGGCGCTTGGGCTACGCCAG
CCGTTTCTTCCTGCTGACCTTGATCAAGTCCGGGACCAGTCTGCGCCGCTTTACCCTGGTGATACGGGAGCTTTATTTTA
CCGGAGTACAGTCGCTCATCATCATCCTCGTGTCAGGGCTTTTTGTCGGCATGGTACTCGGCTTGCAAGGCTTCGAAACC
TTGCAGCGCTATGGAGCAGAATCTGCGGTAGGCACCATGGTGGCGTTGTCATTGCTACGCGAATTGGGGCCTGTAGTGTC
CGCGCTGTTGTTCGCCAGCCGCGCCGGTTCGGCAATCACAGCGGAAATCGGGCTGATGAAAGCGACCGAGCAACTGGCGG
CAATGGAAATGATGGCTGTAGATCCGATTGCGCGCGTAGTCGCGCCCCGGTTCTGGGCGGGCGTGATTTCAATGCCGTTG
CTGGCAGCATTGTTTTCCGCCATAGGCATCTATGGCGGCTATCTGGTGGCTGTCGTACTTATCGGAGTGGATGAAGGTTC
GTTCTGGTCGCAAATGCAAAGCGCCGTCGATTTCCGCTACGATGTGGTCAACGGCATCATAAAAACCTGTGTTTTCGGCA
TCGCGGTGACGGCAATCGCCGTGTTCGAGGGGTATGATGCGGCGCCCACGGCCGAGGGCGTATCCGGTGCCACAACCCGC
ACCGTGGTGACTTCTTCACTCGCAATTCTTGGACTCGATTTTATCTTGACCTCGTTCATGTTCAGGGGAATGGGCTGA

Upstream 100 bases:

>100_bases
CCGTTTGTACATCAGTTCGTTCATGGGGAAGAAGACGGTCCCGTCTCGTTTCACTATCCCGCCAGGCCCTATGCGCAGGA
TCTGAAACTGGAGGGCAGTT

Downstream 100 bases:

>100_bases
TGCAGCGCGCAACAATGGATTTGTGGGTAGGACTGTTCGTCATGGCAGGTATCGGCGCATTGCTCGTGCTGGCGTTGAAA
GTCGGCAATCTTGGTACTTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MPRRIVTGIQSVGHRVIDSIWRLGYASRFFLLTLIKSGTSLRRFTLVIRELYFTGVQSLIIILVSGLFVGMVLGLQGFET
LQRYGAESAVGTMVALSLLRELGPVVSALLFASRAGSAITAEIGLMKATEQLAAMEMMAVDPIARVVAPRFWAGVISMPL
LAALFSAIGIYGGYLVAVVLIGVDEGSFWSQMQSAVDFRYDVVNGIIKTCVFGIAVTAIAVFEGYDAAPTAEGVSGATTR
TVVTSSLAILGLDFILTSFMFRGMG

Sequences:

>Translated_265_residues
MPRRIVTGIQSVGHRVIDSIWRLGYASRFFLLTLIKSGTSLRRFTLVIRELYFTGVQSLIIILVSGLFVGMVLGLQGFET
LQRYGAESAVGTMVALSLLRELGPVVSALLFASRAGSAITAEIGLMKATEQLAAMEMMAVDPIARVVAPRFWAGVISMPL
LAALFSAIGIYGGYLVAVVLIGVDEGSFWSQMQSAVDFRYDVVNGIIKTCVFGIAVTAIAVFEGYDAAPTAEGVSGATTR
TVVTSSLAILGLDFILTSFMFRGMG
>Mature_264_residues
PRRIVTGIQSVGHRVIDSIWRLGYASRFFLLTLIKSGTSLRRFTLVIRELYFTGVQSLIIILVSGLFVGMVLGLQGFETL
QRYGAESAVGTMVALSLLRELGPVVSALLFASRAGSAITAEIGLMKATEQLAAMEMMAVDPIARVVAPRFWAGVISMPLL
AALFSAIGIYGGYLVAVVLIGVDEGSFWSQMQSAVDFRYDVVNGIIKTCVFGIAVTAIAVFEGYDAAPTAEGVSGATTRT
VVTSSLAILGLDFILTSFMFRGMG

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=257, Percent_Identity=58.7548638132296, Blast_Score=301, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28297; Mature: 28166

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRRIVTGIQSVGHRVIDSIWRLGYASRFFLLTLIKSGTSLRRFTLVIRELYFTGVQSLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IILVSGLFVGMVLGLQGFETLQRYGAESAVGTMVALSLLRELGPVVSALLFASRAGSAIT
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AEIGLMKATEQLAAMEMMAVDPIARVVAPRFWAGVISMPLLAALFSAIGIYGGYLVAVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGVDEGSFWSQMQSAVDFRYDVVNGIIKTCVFGIAVTAIAVFEGYDAAPTAEGVSGATTR
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
TVVTSSLAILGLDFILTSFMFRGMG
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]