Click here to switch to the map view.

The map label for this gene is tolB [H]

Identifier: 82703827

GI number: 82703827

Start: 3115165

End: 3116490

Strand: Reverse

Name: tolB [H]

Synonym: Nmul_A2714

Alternate gene names: 82703827

Gene position: 3116490-3115165 (Counterclockwise)

Preceding gene: 82703828

Following gene: 82703826

Centisome position: 97.87

GC content: 56.64

Gene sequence:

>1326_bases
ATGGCCATTGCTATGCTGCCCACGCCGTCCCGCTCGCATAAACTTTCCGGCTATGCTGCCGTTTTGTTCTTTTTGTGGCT
GGTCTGCTCGCCAGCGCAGGCCGCTCTCAACATCGAAATTTTCGGTGGAGGCGCCAACCAGATTCCTGTCGCGATAGTTC
CCTTTTCGGGAGAGGAAGGACTTGGGGCGCAAAGCCTTACCCCCGTCATTTCGGCGGATCTGCAACGTACAGGATTATTT
AAGCTGGTCGATCCCGGAGGCCTGCGGCCGCATGAACCGGTCGAGGTTTCGTTCCCGGATTGGATCAATCGGGGTGCGAG
CGCGCTCGCCATTGGCACCGCGATACCCGTCTCCGGAGGCCAGATATCGATCCGCGTCCGGTTGCTGGATGTGGCAAGGC
AGGCAGAACTGGGAAGTTATGTCGAGACAGTGGCGCCGGAGCAGCTGCGCGCTGCCGCCCACCGCATTGCCGACATGATC
TACGAAAAACTGACCGGTGACGTCGGCGTGTTCAGCACTCGCATTGCCTATGTGATCAAGCAGGGGAAGAAATACTCGCT
CCAGGTGGCAGATGCGGACGGGTTCAATGCCCAGCCCGTCATCGAATATACGGAGCCGATCATATCGCCCGCCTGGTCCC
CGGACGGCACCCGGCTTGCATATGTATCTTTCGAGAACAAGAAACCGGTGGTTTACGTTCAGACCCTTGCGACCCGGACA
CGCAAGGCGGTTGCAAATTTCAGGGGGAGCAATAGTGCGCCGGCATGGTCTCCCGATGGCAGGAAGCTTGCGGTTGTGCT
ATCTGTAATGGGTGGCTCGCAAATCTTTCTGATCAACTCCGATGGCGGAGGACTTCAGCGCCTTACACAAAGTTCCGGCA
TCGATACCGAGCCAAGTTTTTCGCCGGACGGCCGTTATATCATTTTCACCTCTGACCGTGGCGGCAGTCCTCAAATTTAT
CGGATGCCTGTCACAGGAGGTGTGTCCAAAGTTGCGGAACGTCTTACTTTCGAAGGAAGCTACAACGTGAGTCCGCGTTA
CAGTCCCGATGGAAAAGGTTTTACTTTTATCCACCGCAGCGGCGATAGATTCAACGTGGCCATTCAGGACTTTGCGACCG
GCCAGGTGCGCTTGCTCACGGATTCGAGATTCGACGAATCGCCAAGCTTTGCCCCCAACGGCAGAATGGTTCTTTATGCT
ACTGAAATACGGGGCCGTGGTATATTATCCGCGGTTTCGAGTGACGGGAGAACCCGGCAGCAGCTTTCAACGCAGACGGG
TGATATAAGAGAACCGGCGTGGGGTCCGCTACGGCGTCTGCAGTAA

Upstream 100 bases:

>100_bases
CTGCCGCCCGACCCCGCGCTTTTTCCGAAGTTCCGCAACTTGAGTGTCAAGGTGCATTACCTTGAATGACTGGTATAATC
TATTGTTTCAAAATTAACTT

Downstream 100 bases:

>100_bases
TTCCAGTGTATTTTTGGCTAACCTAAAAGGGAATAGAAATGAAAAAAGTTTTGGGCGTGTTATTGATCAGCCTGTTGACA
GCATGCGCCAGCCAGACAAA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MAIAMLPTPSRSHKLSGYAAVLFFLWLVCSPAQAALNIEIFGGGANQIPVAIVPFSGEEGLGAQSLTPVISADLQRTGLF
KLVDPGGLRPHEPVEVSFPDWINRGASALAIGTAIPVSGGQISIRVRLLDVARQAELGSYVETVAPEQLRAAAHRIADMI
YEKLTGDVGVFSTRIAYVIKQGKKYSLQVADADGFNAQPVIEYTEPIISPAWSPDGTRLAYVSFENKKPVVYVQTLATRT
RKAVANFRGSNSAPAWSPDGRKLAVVLSVMGGSQIFLINSDGGGLQRLTQSSGIDTEPSFSPDGRYIIFTSDRGGSPQIY
RMPVTGGVSKVAERLTFEGSYNVSPRYSPDGKGFTFIHRSGDRFNVAIQDFATGQVRLLTDSRFDESPSFAPNGRMVLYA
TEIRGRGILSAVSSDGRTRQQLSTQTGDIREPAWGPLRRLQ

Sequences:

>Translated_441_residues
MAIAMLPTPSRSHKLSGYAAVLFFLWLVCSPAQAALNIEIFGGGANQIPVAIVPFSGEEGLGAQSLTPVISADLQRTGLF
KLVDPGGLRPHEPVEVSFPDWINRGASALAIGTAIPVSGGQISIRVRLLDVARQAELGSYVETVAPEQLRAAAHRIADMI
YEKLTGDVGVFSTRIAYVIKQGKKYSLQVADADGFNAQPVIEYTEPIISPAWSPDGTRLAYVSFENKKPVVYVQTLATRT
RKAVANFRGSNSAPAWSPDGRKLAVVLSVMGGSQIFLINSDGGGLQRLTQSSGIDTEPSFSPDGRYIIFTSDRGGSPQIY
RMPVTGGVSKVAERLTFEGSYNVSPRYSPDGKGFTFIHRSGDRFNVAIQDFATGQVRLLTDSRFDESPSFAPNGRMVLYA
TEIRGRGILSAVSSDGRTRQQLSTQTGDIREPAWGPLRRLQ
>Mature_440_residues
AIAMLPTPSRSHKLSGYAAVLFFLWLVCSPAQAALNIEIFGGGANQIPVAIVPFSGEEGLGAQSLTPVISADLQRTGLFK
LVDPGGLRPHEPVEVSFPDWINRGASALAIGTAIPVSGGQISIRVRLLDVARQAELGSYVETVAPEQLRAAAHRIADMIY
EKLTGDVGVFSTRIAYVIKQGKKYSLQVADADGFNAQPVIEYTEPIISPAWSPDGTRLAYVSFENKKPVVYVQTLATRTR
KAVANFRGSNSAPAWSPDGRKLAVVLSVMGGSQIFLINSDGGGLQRLTQSSGIDTEPSFSPDGRYIIFTSDRGGSPQIYR
MPVTGGVSKVAERLTFEGSYNVSPRYSPDGKGFTFIHRSGDRFNVAIQDFATGQVRLLTDSRFDESPSFAPNGRMVLYAT
EIRGRGILSAVSSDGRTRQQLSTQTGDIREPAWGPLRRLQ

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=421, Percent_Identity=36.8171021377672, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 47570; Mature: 47438

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAMLPTPSRSHKLSGYAAVLFFLWLVCSPAQAALNIEIFGGGANQIPVAIVPFSGEEG
CEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEECCCCCC
LGAQSLTPVISADLQRTGLFKLVDPGGLRPHEPVEVSFPDWINRGASALAIGTAIPVSGG
CCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCEEEECHHHHCCCCCEEEEEEEEECCCC
QISIRVRLLDVARQAELGSYVETVAPEQLRAAAHRIADMIYEKLTGDVGVFSTRIAYVIK
EEEEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
QGKKYSLQVADADGFNAQPVIEYTEPIISPAWSPDGTRLAYVSFENKKPVVYVQTLATRT
CCCEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHH
RKAVANFRGSNSAPAWSPDGRKLAVVLSVMGGSQIFLINSDGGGLQRLTQSSGIDTEPSF
HHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHCCCCCCCCC
SPDGRYIIFTSDRGGSPQIYRMPVTGGVSKVAERLTFEGSYNVSPRYSPDGKGFTFIHRS
CCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHEECCCCCCCCCCCCCCCCEEEEEEC
GDRFNVAIQDFATGQVRLLTDSRFDESPSFAPNGRMVLYATEIRGRGILSAVSSDGRTRQ
CCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEHHCCCCHHHH
QLSTQTGDIREPAWGPLRRLQ
HHHCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
AIAMLPTPSRSHKLSGYAAVLFFLWLVCSPAQAALNIEIFGGGANQIPVAIVPFSGEEG
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEECCCCCC
LGAQSLTPVISADLQRTGLFKLVDPGGLRPHEPVEVSFPDWINRGASALAIGTAIPVSGG
CCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCEEEECHHHHCCCCCEEEEEEEEECCCC
QISIRVRLLDVARQAELGSYVETVAPEQLRAAAHRIADMIYEKLTGDVGVFSTRIAYVIK
EEEEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
QGKKYSLQVADADGFNAQPVIEYTEPIISPAWSPDGTRLAYVSFENKKPVVYVQTLATRT
CCCEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEHHHHHH
RKAVANFRGSNSAPAWSPDGRKLAVVLSVMGGSQIFLINSDGGGLQRLTQSSGIDTEPSF
HHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHCCCCCCCCC
SPDGRYIIFTSDRGGSPQIYRMPVTGGVSKVAERLTFEGSYNVSPRYSPDGKGFTFIHRS
CCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHEECCCCCCCCCCCCCCCCEEEEEEC
GDRFNVAIQDFATGQVRLLTDSRFDESPSFAPNGRMVLYATEIRGRGILSAVSSDGRTRQ
CCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEHHCCCCHHHH
QLSTQTGDIREPAWGPLRRLQ
HHHCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA