Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 82703806

GI number: 82703806

Start: 3095547

End: 3096455

Strand: Reverse

Name: pleD [H]

Synonym: Nmul_A2693

Alternate gene names: 82703806

Gene position: 3096455-3095547 (Counterclockwise)

Preceding gene: 82703807

Following gene: 82703803

Centisome position: 97.24

GC content: 53.8

Gene sequence:

>909_bases
ATGAAGATACTCATTGCCGAAGACGACACGACCTCGCGCCTGTTGTTTGCCGCCACCCTCAGGAAACTGGGTCATACCGT
AACGGCAGTAGAGGATGGGCGTAAGGCATGGGAAGCCTGGCAAAAGGATGAATATCCGCTGCTCATCTCCGACTGGATGA
TGCCTGATATCGATGGTCTGCAATTATGCAGAATGATTCGCGGCGAACCGGGCCTGCAATATACCTACATCATCCTTCTT
ACTGCGCTGGACAGCAAGGGCAGCTATCTCGAAGGCATGGACGCAGGGGCAGATGATTTCATTACTAAACCGTTCGATGA
AGAACAGCTGGCAGCCCGGCTACGGGTGGCTGAACGAATACTTGGCCTGCATCAAAAGCTACATATCCAGGCCACGCGGG
ATTATTTGACCGGCGTATGGAACCGAGCCTCCATCATGGATTATCTGCAGAAGGAGTTGAAGCGAGGAGCGCGGCAGGAT
ATCTGCACGAGTGTGATGATAGCGGATCTCGATCACTTCAAGCGAATCAACGATACCTATGGGCATCCGACCGGTGATGA
AATACTGCAAGAGGCAGCCCGCCGTATGTCAGGTGTATTACGGGGTTATGACCGGGTCGGACGCTATGGAGGCGAGGAAT
TTCTCATTACGGCATCGGGTTGTGACTGGAGCGAGGCCCTGGCCCTGGGCGAAAGAATCTGTAGCTCCATCAGGAGCGAG
CCTGCCAACAGCCGTGCGGGACAAATTGACATGACTGTGAGCATAGGTATCGCGACGAGTTGCGGACAGGTTAATGATAT
CGGTGAGCTTATCGCAGCCGCCGATCAAGCGCTCTATCAAGCAAAAGAGGCTGGGCGTAATCAAGTTAAATTGTCGCGGT
TGCCGATCGAATCAGGACCCGCGCCATAG

Upstream 100 bases:

>100_bases
TTACTTTAATTGAGCAAATTGAAATGGAATTTGGACGCGTGCAGCATGAAATTACCCAGTCAGAGCTCGATTGCGTAGCG
CTGCCGGATGCAACTCGACC

Downstream 100 bases:

>100_bases
CGCCGAAAAATTGCCGCCTCCGGCTCTATTGGAGCGTCATCGGCTCTTACGTTTTCACCACGTAGAGTGTGGGACAAGGC
TGCGGTAATTTTTAGCTCTG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKILIAEDDTTSRLLFAATLRKLGHTVTAVEDGRKAWEAWQKDEYPLLISDWMMPDIDGLQLCRMIRGEPGLQYTYIILL
TALDSKGSYLEGMDAGADDFITKPFDEEQLAARLRVAERILGLHQKLHIQATRDYLTGVWNRASIMDYLQKELKRGARQD
ICTSVMIADLDHFKRINDTYGHPTGDEILQEAARRMSGVLRGYDRVGRYGGEEFLITASGCDWSEALALGERICSSIRSE
PANSRAGQIDMTVSIGIATSCGQVNDIGELIAAADQALYQAKEAGRNQVKLSRLPIESGPAP

Sequences:

>Translated_302_residues
MKILIAEDDTTSRLLFAATLRKLGHTVTAVEDGRKAWEAWQKDEYPLLISDWMMPDIDGLQLCRMIRGEPGLQYTYIILL
TALDSKGSYLEGMDAGADDFITKPFDEEQLAARLRVAERILGLHQKLHIQATRDYLTGVWNRASIMDYLQKELKRGARQD
ICTSVMIADLDHFKRINDTYGHPTGDEILQEAARRMSGVLRGYDRVGRYGGEEFLITASGCDWSEALALGERICSSIRSE
PANSRAGQIDMTVSIGIATSCGQVNDIGELIAAADQALYQAKEAGRNQVKLSRLPIESGPAP
>Mature_302_residues
MKILIAEDDTTSRLLFAATLRKLGHTVTAVEDGRKAWEAWQKDEYPLLISDWMMPDIDGLQLCRMIRGEPGLQYTYIILL
TALDSKGSYLEGMDAGADDFITKPFDEEQLAARLRVAERILGLHQKLHIQATRDYLTGVWNRASIMDYLQKELKRGARQD
ICTSVMIADLDHFKRINDTYGHPTGDEILQEAARRMSGVLRGYDRVGRYGGEEFLITASGCDWSEALALGERICSSIRSE
PANSRAGQIDMTVSIGIATSCGQVNDIGELIAAADQALYQAKEAGRNQVKLSRLPIESGPAP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=168, Percent_Identity=35.1190476190476, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI145693134, Length=179, Percent_Identity=37.9888268156425, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=36.6459627329193, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI87082007, Length=178, Percent_Identity=36.5168539325843, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787802, Length=214, Percent_Identity=29.4392523364486, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=37.4269005847953, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787541, Length=167, Percent_Identity=34.1317365269461, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=30.1675977653631, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI87081977, Length=207, Percent_Identity=31.8840579710145, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082012, Length=119, Percent_Identity=37.8151260504202, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788381, Length=167, Percent_Identity=30.5389221556886, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=126, Percent_Identity=35.7142857142857, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788191, Length=124, Percent_Identity=32.258064516129, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790552, Length=122, Percent_Identity=38.5245901639344, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=33.0508474576271, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786784, Length=123, Percent_Identity=34.1463414634146, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788956, Length=160, Percent_Identity=34.375, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=29.6610169491525, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787375, Length=120, Percent_Identity=34.1666666666667, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789149, Length=110, Percent_Identity=30, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789402, Length=119, Percent_Identity=36.1344537815126, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33526; Mature: 33526

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIAEDDTTSRLLFAATLRKLGHTVTAVEDGRKAWEAWQKDEYPLLISDWMMPDIDGL
CEEEEECCCCHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHH
QLCRMIRGEPGLQYTYIILLTALDSKGSYLEGMDAGADDFITKPFDEEQLAARLRVAERI
HHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LGLHQKLHIQATRDYLTGVWNRASIMDYLQKELKRGARQDICTSVMIADLDHFKRINDTY
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GHPTGDEILQEAARRMSGVLRGYDRVGRYGGEEFLITASGCDWSEALALGERICSSIRSE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCC
PANSRAGQIDMTVSIGIATSCGQVNDIGELIAAADQALYQAKEAGRNQVKLSRLPIESGP
CCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
AP
CC
>Mature Secondary Structure
MKILIAEDDTTSRLLFAATLRKLGHTVTAVEDGRKAWEAWQKDEYPLLISDWMMPDIDGL
CEEEEECCCCHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHH
QLCRMIRGEPGLQYTYIILLTALDSKGSYLEGMDAGADDFITKPFDEEQLAARLRVAERI
HHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LGLHQKLHIQATRDYLTGVWNRASIMDYLQKELKRGARQDICTSVMIADLDHFKRINDTY
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GHPTGDEILQEAARRMSGVLRGYDRVGRYGGEEFLITASGCDWSEALALGERICSSIRSE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCC
PANSRAGQIDMTVSIGIATSCGQVNDIGELIAAADQALYQAKEAGRNQVKLSRLPIESGP
CCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
AP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA