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The map label for this gene is miaB

Identifier: 82703805

GI number: 82703805

Start: 3094171

End: 3095595

Strand: Direct

Name: miaB

Synonym: Nmul_A2692

Alternate gene names: 82703805

Gene position: 3094171-3095595 (Clockwise)

Preceding gene: 82703804

Following gene: 82703809

Centisome position: 97.17

GC content: 54.04

Gene sequence:

>1425_bases
GTGGCAAAAAAACTTTACATCAAGACATTTGGCTGCCAGATGAACGAGTATGACTCGAAAAAGATGGCGGATGTGCTGCG
TGATGCGCAGCACATGGAAAAAACGGATGATCCCGCCGCGGCTGACGTAATTCTTTTCAACACCTGCTCGGTGCGGGAAA
AAGCGCAGGAAAAGGTGTTTCATGACCTTGGGCGCGTAAGACATCTCAAGGCAGCCAATCCCGATCTGCTGATAGGTGTG
GGCGGATGCGTTGCCAGCCAGGAAGGGGCGGAGATCGTCAAACGCGCGCCTTATGTAGACCTCGTGTTTGGTCCGCAGAC
GCTGCACCGGCTGCCGCAGATGATCTCAACGCGTCAGATTACGGGCCGTCCGCAGGTGGATATCTCCTTTCCGGAGATCG
AGAAATTCGATCATCTGCCCCCGGCGCGCACGGAGGGCGTAACCGCCTTCGTATCCATCATGGAAGGGTGCAGCAAGTAT
TGCAGCTTTTGTGTGGTTCCTTATACGCGCGGTGAGGAAATTTCACGTCCGCTCGACGATATCCTGACGGAAATCGCGGG
TCTGACAAATCTGGGGGTCAAGGAAGTAACCCTGCTGGGGCAAAACGTCAACGCCTATCGCGGCAGGATGCAATATGCGG
AAGAGGGCGAACTAGCAGACTTTGCGCTGTTGCTGGAATATCTCCATGAAATCCCGGGTATCGAACGCATCCGTTATACC
ACCTCTCATCCGAGGGAATTCACGCCTCGCCTCATCGAAGCTTACAAGGCTTCCCCCAAGCTGGTAAGTCACGTGCATTT
GCCGGTTCAGTCCGGTTCGGATCGGATCCTCGCAGCCATGAAGCGAGGCTATACTTCGCTGGAATACAAATCGATCATTC
GCCGGCTGCGTGCTGCCCGACCAGACATTTCGATTACCTCTGATTTCATTGTCGGTTTTCCCGGGGAAACCGAAGCAGAC
TTTGAGGCAACGATGAAACTGATCGAGGCGGTAAACTTTGATGGTTCCTTCAGCTTTATTTACAGTTCCCGTCCGGGGAC
TCCGGCAGCCGGATTGGAAGATACTACGCCGCATCAGGTCAAACTGGAGCGGCTGCAGCGGTTACAGGAAAAAGTGGAGC
TACAGGCGCAAGCGATCAGCGTCAGGATGGTTGGGACGACACAACGCGTTCTGGTGGAAGGCTTGTCCAGAAAAGATCCC
GGCGAATTGAGCGGCCGCACCGACAATAACCGTGTGGTCAATTTTCCCGGTTCTCCCGAAATGATAGGAAAATTTGCAGA
GCTAAAAATTACCGCAGCCTTGTCCCACACTCTACGTGGTGAAAACGTAAGAGCCGATGACGCTCCAATAGAGCCGGAGG
CGGCAATTTTTCGGCGCTATGGCGCGGGTCCTGATTCGATCGGCAACCGCGACAATTTAACTTGA

Upstream 100 bases:

>100_bases
AACAGCGGGTAACTCCGGGCAGGAACGATATCCCGGGGCACCTCTGAATTGCAGATTGCCTTGGTGCTCATCATGATTTA
CTGAAACAGCAAGTAAGGCC

Downstream 100 bases:

>100_bases
TTACGCCCAGCCTCTTTTGCTTGATAGAGCGCTTGATCGGCGGCTGCGATAAGCTCACCGATATCATTAACCTGTCCGCA
ACTCGTCGCGATACCTATGC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MAKKLYIKTFGCQMNEYDSKKMADVLRDAQHMEKTDDPAAADVILFNTCSVREKAQEKVFHDLGRVRHLKAANPDLLIGV
GGCVASQEGAEIVKRAPYVDLVFGPQTLHRLPQMISTRQITGRPQVDISFPEIEKFDHLPPARTEGVTAFVSIMEGCSKY
CSFCVVPYTRGEEISRPLDDILTEIAGLTNLGVKEVTLLGQNVNAYRGRMQYAEEGELADFALLLEYLHEIPGIERIRYT
TSHPREFTPRLIEAYKASPKLVSHVHLPVQSGSDRILAAMKRGYTSLEYKSIIRRLRAARPDISITSDFIVGFPGETEAD
FEATMKLIEAVNFDGSFSFIYSSRPGTPAAGLEDTTPHQVKLERLQRLQEKVELQAQAISVRMVGTTQRVLVEGLSRKDP
GELSGRTDNNRVVNFPGSPEMIGKFAELKITAALSHTLRGENVRADDAPIEPEAAIFRRYGAGPDSIGNRDNLT

Sequences:

>Translated_474_residues
MAKKLYIKTFGCQMNEYDSKKMADVLRDAQHMEKTDDPAAADVILFNTCSVREKAQEKVFHDLGRVRHLKAANPDLLIGV
GGCVASQEGAEIVKRAPYVDLVFGPQTLHRLPQMISTRQITGRPQVDISFPEIEKFDHLPPARTEGVTAFVSIMEGCSKY
CSFCVVPYTRGEEISRPLDDILTEIAGLTNLGVKEVTLLGQNVNAYRGRMQYAEEGELADFALLLEYLHEIPGIERIRYT
TSHPREFTPRLIEAYKASPKLVSHVHLPVQSGSDRILAAMKRGYTSLEYKSIIRRLRAARPDISITSDFIVGFPGETEAD
FEATMKLIEAVNFDGSFSFIYSSRPGTPAAGLEDTTPHQVKLERLQRLQEKVELQAQAISVRMVGTTQRVLVEGLSRKDP
GELSGRTDNNRVVNFPGSPEMIGKFAELKITAALSHTLRGENVRADDAPIEPEAAIFRRYGAGPDSIGNRDNLT
>Mature_473_residues
AKKLYIKTFGCQMNEYDSKKMADVLRDAQHMEKTDDPAAADVILFNTCSVREKAQEKVFHDLGRVRHLKAANPDLLIGVG
GCVASQEGAEIVKRAPYVDLVFGPQTLHRLPQMISTRQITGRPQVDISFPEIEKFDHLPPARTEGVTAFVSIMEGCSKYC
SFCVVPYTRGEEISRPLDDILTEIAGLTNLGVKEVTLLGQNVNAYRGRMQYAEEGELADFALLLEYLHEIPGIERIRYTT
SHPREFTPRLIEAYKASPKLVSHVHLPVQSGSDRILAAMKRGYTSLEYKSIIRRLRAARPDISITSDFIVGFPGETEADF
EATMKLIEAVNFDGSFSFIYSSRPGTPAAGLEDTTPHQVKLERLQRLQEKVELQAQAISVRMVGTTQRVLVEGLSRKDPG
ELSGRTDNNRVVNFPGSPEMIGKFAELKITAALSHTLRGENVRADDAPIEPEAAIFRRYGAGPDSIGNRDNLT

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=37.1069182389937, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=37.1069182389937, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI93277076, Length=456, Percent_Identity=28.0701754385965, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=65.5480984340045, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI1787057, Length=464, Percent_Identity=27.801724137931, Blast_Score=151, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=459, Percent_Identity=34.4226579520697, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI71996771, Length=317, Percent_Identity=28.7066246056782, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI21356207, Length=476, Percent_Identity=32.563025210084, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI19922432, Length=458, Percent_Identity=27.292576419214, Blast_Score=143, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_NITMU (Q2Y5J2)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413371.1
- ProteinModelPortal:   Q2Y5J2
- SMR:   Q2Y5J2
- STRING:   Q2Y5J2
- GeneID:   3785054
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2692
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q2Y5J2
- ProtClustDB:   PRK14325
- BioCyc:   NMUL323848:NMUL_A2692-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52682; Mature: 52551

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKLYIKTFGCQMNEYDSKKMADVLRDAQHMEKTDDPAAADVILFNTCSVREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHH
HDLGRVRHLKAANPDLLIGVGGCVASQEGAEIVKRAPYVDLVFGPQTLHRLPQMISTRQI
HHHHHHHHHCCCCCCEEEECCCHHCCCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHC
TGRPQVDISFPEIEKFDHLPPARTEGVTAFVSIMEGCSKYCSFCVVPYTRGEEISRPLDD
CCCCCEEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCHHHHCCHHH
ILTEIAGLTNLGVKEVTLLGQNVNAYRGRMQYAEEGELADFALLLEYLHEIPGIERIRYT
HHHHHHCHHCCCHHHEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEC
TSHPREFTPRLIEAYKASPKLVSHVHLPVQSGSDRILAAMKRGYTSLEYKSIIRRLRAAR
CCCCHHHHHHHHHHHCCCCCHHHEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCC
PDISITSDFIVGFPGETEADFEATMKLIEAVNFDGSFSFIYSSRPGTPAAGLEDTTPHQV
CCCEECCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHH
KLERLQRLQEKVELQAQAISVRMVGTTQRVLVEGLSRKDPGELSGRTDNNRVVNFPGSPE
HHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHH
MIGKFAELKITAALSHTLRGENVRADDAPIEPEAAIFRRYGAGPDSIGNRDNLT
HHHHHHHEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKLYIKTFGCQMNEYDSKKMADVLRDAQHMEKTDDPAAADVILFNTCSVREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHH
HDLGRVRHLKAANPDLLIGVGGCVASQEGAEIVKRAPYVDLVFGPQTLHRLPQMISTRQI
HHHHHHHHHCCCCCCEEEECCCHHCCCHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHC
TGRPQVDISFPEIEKFDHLPPARTEGVTAFVSIMEGCSKYCSFCVVPYTRGEEISRPLDD
CCCCCEEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCHHHHCCHHH
ILTEIAGLTNLGVKEVTLLGQNVNAYRGRMQYAEEGELADFALLLEYLHEIPGIERIRYT
HHHHHHCHHCCCHHHEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEC
TSHPREFTPRLIEAYKASPKLVSHVHLPVQSGSDRILAAMKRGYTSLEYKSIIRRLRAAR
CCCCHHHHHHHHHHHCCCCCHHHEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCC
PDISITSDFIVGFPGETEADFEATMKLIEAVNFDGSFSFIYSSRPGTPAAGLEDTTPHQV
CCCEECCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHH
KLERLQRLQEKVELQAQAISVRMVGTTQRVLVEGLSRKDPGELSGRTDNNRVVNFPGSPE
HHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHH
MIGKFAELKITAALSHTLRGENVRADDAPIEPEAAIFRRYGAGPDSIGNRDNLT
HHHHHHHEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA